Rat CXCL1/CINC-1/KC ELISA Kit | CXCL1 elisa kit

Rat CXCL1/CINC-1/KC ELISA Kit

Cat. Num. AAA281391

• Reactivity: Rat
• Synonyms: CXCL1/CINC-1/KC; N/A; Rat CXCL1/CINC-1/KC ELISA Kit; CXCL1 elisa kit

• Reactivity: Rat
• Specificity: This assay recognizes both recombinant and natural rat CXCL1/CINC-1/KC. The factors listed below were prepared at 50ng/ml and assayed for cross-reactivity.
• Sequence Length: 107
• Assay Type: Quantitative Sandwich
• Samples: Cell culture supernates, serum and plasma
• Sensitivity: < 1.95 pg/mL

• Intra-assay Precision: Three samples of known concentration were tested 20 times on one plate to evaluate the Intra-plate precision.
• Inter-assay Precision: Three samples of known concentration were tested 20 times separate assays to evaluate the Inter-plate precision. Assay were using two lots of components.
• Preparation and Storage: Store at 4 degree C.

Standard Curve (Sample)

(Rat CXCL1/CINC-1/KC ELISA Kit)

Related Product Information for CXCL1 elisa kit

Background: Rat Cytokine-Induced Neutrophil Chemoattractant-1 (CINC-1), otherwise known as CXCL1, CINC, rat GROalpha, and rat KC, is an 8 kDa member of the alpha-, or CXC family of chemokines (1-4). It is synthesized as a 96 amino acid (aa) precursor that contains a 24 aa signal sequence plus a 72 aa mature segment (5, 6). In the mature segment, there are two intrachain disulfide bonds plus an ELR and CXC motif. There are no potential N-linked glycosylation sites. The molecule reportedly circulates as a nondisulfide-linked homodimer (1, 5, 7). In the mature region, rat CINC-1 shows 66% and 70% aa sequence identity to rat CINC-2alpha and CINC-3, respectively (1, 5, 8, 9). It shows 92% aa sequence identity to mouse KC and 69% aa sequence identity to human GROalpha (5, 10). Cells known to express CINC-1 are varied and include hepatocytes (11, 12), mast cells (13), macrophages (14-16), neurons (17), Kupffer cells (12), fibroblasts (18), type II greater alveolar cells (19), and cardiac muscle cells (20). The receptor for rat CINC-1 is CXCR2 (21, 22). Although both rat CXCR1-like and CXCR2 have been reported in the literature (22), the CXCR1-like molecule is unresponsive to CXC ligands. Thus, only CXCR2 is considered the functional CINC receptor (21). Functionally, CINC-1 is described as a major neutrophil chemoattractant and activator (1, 13, 23-25). CINC-1, induced by IL-1beta, TNF-alpha and bacterial products, promotes both neutrophil rolling and adhesion, likely through the upregulation of surface integrins (15, 25-28). Thus, it directs neutrophils to sites of bacterially-induced inflammation (27). It is also reported to stimulate neutrophil activity by promoting cathepsin G release from azurophilic granules (23). It does not, however, seem to impact nitrite production (24). Thus it makes a partial contribution to bacterial killing. Relative to CINC-2alpha and CINC-3, CINC-1 seems to be equal in chemotactic activity but less efficient in inducing calcium mobilization (29). It is also induced earlier in macrophages than CINC-2 and -3 and declines more quickly in expression (16). The significance of this is unclear.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for CXCL1/CINC-1/KC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CXCL1/CINC-1/KC present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for CXCL1/CINC-1/KC is added to the wells and binds to the combination of capture antibody- CXCL1/CINC-1/KC in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps, a substrate is added. A colored product TMB is formed in proportion to the amount of CXCL1/CINC-1/KC present in the sample. The reaction is terminated by addition of acid and absorbance is measured. A standard curve is prepared from seven CXCL1/CINC-1/KC standard dilutions and CXCL1/CINC-1/KC sample concentration determined.

NCBI and Uniprot Product Information

• NCBI GI #: 545686986
• NCBI GeneID: 102126138
• NCBI Accession #: NP_001270423.1
• NCBI GenBank Nucleotide #: NM_001283494.1
• NCBI Official Full Name: chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
• NCBI Official Symbol: CXCL1
• NCBI Protein Information: chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)