Mouse MFGE8/Lactadherin ELISA Kit | MFGE8 elisa kit

Mouse MFGE8/Lactadherin PicoKine ELISA Kit

Cat. Num. AAA45821

• Gene Names: Mfge8; P47; MP47; Mfgm; SED1; MFG-E8; AA408458; AI325141; lactadherin
• Reactivity: Mouse
• Synonyms: MFGE8/Lactadherin; N/A; Mouse MFGE8/Lactadherin PicoKine ELISA Kit; Lactadherin; milk fat globule-EGF factor 8 protein; BA46; Breast epithelial antigen BA46; Contains Medin; EDIL1; HMFG; hP47; HsT19888; LactadherinLactadherin [Precursor]; Medin; MFG E8; MFG-E8; MFGE8; MFGM; MFGM_HUMAN; Milk fat globule EGF factor 8; Milk fat globule EGF factor 8 protein; Milk fat globule-EGF factor 8; O acetyl disialoganglioside synthase; OAcGD3S; SED1; SPAG10; Sperm associated antigen 10; Sperm surface protein hP47; MFGE8 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse MFGE8
• Sequence Length: 463
• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: < 10pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Mouse MFGE8/Lactadherin PicoKine ELISA Kit standard curve)

Related Product Information for MFGE8 elisa kit

Principle of the assay: mouse MFGE8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MFGE8 has been precoated onto 96-well plates. Standards(NSO, A23-C463) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MFGE8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MFGE8 amount of sample captured in plate.
Background: MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, the MFGE8 gene was mapped to chromosome 15q25. MFGE8 is a factor that links apoptotic cells to phagocytes. It specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. It showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE)-dependent mechanism.

NCBI and Uniprot Product Information

• NCBI GI #: 341941002
• NCBI GeneID: 17304
• UniProt Accession #: P21956; P97800; Q3TBN5; Q3U8S9; Q9R1X9; Q9WTS3
• Molecular Weight: 47,169 Da
• NCBI Official Full Name: Lactadherin
• NCBI Official Synonym Full Names: milk fat globule-EGF factor 8 protein
• NCBI Official Symbol: Mfge8
• NCBI Official Synonym Symbols: P47; MP47; Mfgm; SED1; MFG-E8; AA408458; AI325141; lactadherin
• NCBI Protein Information: lactadherin; EGF/factor VIII; sperm surface protein SP47; milk fat globule glycoprotein
• UniProt Protein Name: Lactadherin
• UniProt Gene Name: Mfge8
• UniProt Synonym Gene Names: MFG-E8; MP47
• UniProt Entry Name: MFGM_MOUSE