WB (Western Blot)
(Detection of human and mouse Timeless by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Timeless antibody AAA211250 (lot AAA211250 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human Timeless Polyclonal Antibody | anti-TIMELESS antibody
Rabbit anti-Timeless Antibody, Affinity Purified
Gene Names
TIMELESS; TIM; TIM1; hTIM
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Timeless, Antibody; Rabbit anti-Timeless Antibody, Affinity Purified; timeless circadian clock 1; hTIM; timeless homolog; TIM1; TIM; protein timeless homolog; Tof1 homolog; Protein timeless homolog; timeless circadian regulator; anti-TIMELESS antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-TIMELESS antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1158 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse Timeless by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Timeless antibody AAA211250 (lot AAA211250 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human and mouse Timeless by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Timeless antibody AAA211250 (lot AAA211250 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human Timeless by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Timeless antibody AAA211250 (lot AAA211250-3) used for IP at 3 ug per reaction. Timeless was also immunoprecipitated by rabbit anti-Timeless antibody For blotting immunoprecipitated Timeless, AAA211250 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human Timeless by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Timeless antibody AAA211250 (lot AAA211250-3) used for IP at 3 ug per reaction. Timeless was also immunoprecipitated by rabbit anti-Timeless antibody For blotting immunoprecipitated Timeless, AAA211250 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IHC (Immunohistochemistry)
(Detection of human Timeless by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Timeless (Cat. No. AAA211250 Lot3) used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Timeless by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Timeless (Cat. No. AAA211250 Lot3) used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-TIMELESS antibody
Timeless was first identified in Drosophila as a transcriptional regulator involved in the fly circadian rhythm. In mice, humans and yeast, the homologs of drosophila timeless have been observed to play a role in the DNA damage checkpoint response. Human timeless has been shown to interact with the circadian clock protein cryptochrome 2 as well as the DNA checkpoint proteins Chk1 and the ATR-ATRIP complex suggesting a link between circadian rhythm and the cell cycle.
Product Categories/Family for anti-TIMELESS antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
138,658 Da
NCBI Official Full Name
protein timeless homolog
NCBI Official Synonym Full Names
timeless circadian clock
NCBI Official Symbol
TIMELESS
NCBI Official Synonym Symbols
TIM; TIM1; hTIM
NCBI Protein Information
protein timeless homolog; Tof1 homolog; timeless circadian clock 1
UniProt Protein Name
Protein timeless homolog
UniProt Gene Name
TIMELESS
UniProt Synonym Gene Names
hTIM
UniProt Entry Name
TIM_HUMAN
Similar Products
Product Notes
The TIMELESS timeless (Catalog #AAA211250) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Timeless Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Timeless can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the TIMELESS timeless for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Timeless, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.