Standard Curve (Sample)
(Fig.1. Mouse Transmembrane and ubiquitin-like domain-containing protein 1 (TMUB1) Standard Curve.)
Mouse Transmembrane And Ubiquitin-Like Domain-Containing Protein 1 (TMUB1) ELISA Kit | TMUB1 elisa kit
Mouse Transmembrane And Ubiquitin-Like Domain-Containing Protein 1 (TMUB1) ELISA Kit
Gene Names
Tmub1; Hops; AB030183; 2010004O20Rik
Reactivity
Mouse
Synonyms
Transmembrane And Ubiquitin-Like Domain-Containing Protein 1 (TMUB1); N/A; Mouse Transmembrane And Ubiquitin-Like Domain-Containing Protein 1 (TMUB1) ELISA Kit; TMUB1; C7orf21; MGC5442; SB144; TMUB1 elisa kit
Reactivity
Mouse
Specificity
Mouse Transmembrane and ubiquitin-like domain-containing protein 1 (TMUB1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMUB1. No significant cross-reactivity or interference between Mouse TMUB1 and analogues was observed.
Sequence Length
245
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Mouse Transmembrane and ubiquitin-like domain-containing protein 1 (TMUB1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Mouse Transmembrane and ubiquitin-like domain-containing protein 1 (TMUB1) Standard Curve.)
Related Product Information for TMUB1 elisa kit
Description: This Mouse Transmembrane and ubiquitin-like domain-containing protein 1 (TMUB1) ELISA Kit employs a two-site sandwich ELISA to quantitate TMUB1 in samples. An antibody specific for TMUB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMUB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMUB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMUB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Tmub1 encodes for a protein, HOPS/DULP, containing transmembrane domains and a ubiquitin-like domain (UBL) Ubiquitin can be employed to modify proteins by either single or multiple ubiquitin conjugation to mark them for proteasomal destruction, endocytosis and binding to other proteins. Tmub1/HOPS was reported to facilitate the recycling of AMPA receptors into synaptic membrane in cultured primary neurons. Tmub1/HOPS was also found overexpressed during liver regeneration and is an essential constituent of centrosome assembly during cell cycles in culture. Like many UBL domain proteins, the physiological and molecular function of Tmub1 is far from being clear. The resulting mice Tmub1were put through an extensive battery of assays to discover physiological and behavioral phenotypes.
Background: Tmub1 encodes for a protein, HOPS/DULP, containing transmembrane domains and a ubiquitin-like domain (UBL) Ubiquitin can be employed to modify proteins by either single or multiple ubiquitin conjugation to mark them for proteasomal destruction, endocytosis and binding to other proteins. Tmub1/HOPS was reported to facilitate the recycling of AMPA receptors into synaptic membrane in cultured primary neurons. Tmub1/HOPS was also found overexpressed during liver regeneration and is an essential constituent of centrosome assembly during cell cycles in culture. Like many UBL domain proteins, the physiological and molecular function of Tmub1 is far from being clear. The resulting mice Tmub1were put through an extensive battery of assays to discover physiological and behavioral phenotypes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
transmembrane and ubiquitin-like domain-containing protein 1
NCBI Official Synonym Full Names
transmembrane and ubiquitin-like domain containing 1
NCBI Official Symbol
Tmub1
NCBI Official Synonym Symbols
Hops; AB030183; 2010004O20Rik
NCBI Protein Information
transmembrane and ubiquitin-like domain-containing protein 1
UniProt Protein Name
Transmembrane and ubiquitin-like domain-containing protein 1
UniProt Gene Name
Tmub1
UniProt Synonym Gene Names
Hops
UniProt Entry Name
TMUB1_MOUSE
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Product Notes
The Mouse TMUB1 tmub1 (Catalog #AAA332156) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332156 ELISA Kit recognizes Mouse TMUB1. It is sometimes possible for the material contained within the vial of "Transmembrane And Ubiquitin-Like Domain-Containing Protein 1 (TMUB1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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