Standard Curve (Sample)
Mouse TRKC ELISA Kit | TRKC elisa kit
Mouse TRKC PicoKine ELISA Kit
Gene Names
NTRK3; TRKC; GP145-TrkC; gp145(trkC)
Reactivity
Mouse
Synonyms
TRKC; N/A; Mouse TRKC PicoKine ELISA Kit; Mouse TRKC; TRKC elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Ntrk3
Sequence Length
839
Samples
Cell culture supernatants and cell lysates
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TRKC elisa kit
Background: NTRK3 (Neurotrophic Tyrosine Kinase Receptor Type 3), also known as TRKC, is a protein that in humans is encoded by the NTRK3 gene. By PCR analysis of a somatic cell hybrid panel and by fluorescence in situ hybridization with the cDNA clone, it mapped the NTRK3 gene to 15q24-q25. TRKC, a member of the TRK family of tyrosine protein kinase genes. They found that TRKC is preferentially expressed in the brain; in situ hybridization studies showed transcripts in the hippocampus, cerebral cortex, and the granular cell layer of the cerebellum. By functional studies in HeLa cells, 5 miRNAs regulate the truncated form of NTRK3.
Principle of the Assay: The Picokine™ Mouse Ntrk3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Ntrk3 with a 96-well strip plate that is pre-coated with antibody specific for Ntrk3. The detection antibody is a biotinylated antibody specific for Ntrk3. The capture antibody is a monoclonal antibody from rat and the detection antibody is a biotinylated polyclonal antibody from goat. The kit includes Mouse Ntrk3 protein as standards. To measure Mouse Ntrk3, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Ntrk3 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Ntrk3 in the sample.
Principle of the Assay: The Picokine™ Mouse Ntrk3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Ntrk3 with a 96-well strip plate that is pre-coated with antibody specific for Ntrk3. The detection antibody is a biotinylated antibody specific for Ntrk3. The capture antibody is a monoclonal antibody from rat and the detection antibody is a biotinylated polyclonal antibody from goat. The kit includes Mouse Ntrk3 protein as standards. To measure Mouse Ntrk3, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Ntrk3 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Ntrk3 in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
TRKC
NCBI Official Synonym Full Names
neurotrophic receptor tyrosine kinase 3
NCBI Official Symbol
NTRK3
NCBI Official Synonym Symbols
TRKC; GP145-TrkC; gp145(trkC)
NCBI Protein Information
NT-3 growth factor receptor
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Product Notes
The Mouse TRKC (Catalog #AAA125004) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA125004 ELISA Kit recognizes Mouse TRKC. It is sometimes possible for the material contained within the vial of "TRKC, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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