FCM/FACS (Flow Cytometry)
(Anti-Hu CD270 Biotin antibody (clone CW10) specificity verification by flow cytometry Anti-Hu CD270 Biotin antibody (concentration in sample 5ug/ml, streptavidin APC, red-filled histogram) binds specifically to CD270 on surface of SK-MEL-30 cells (upper panel) and also main leukocyte subpopulations - neutrophil granulocytes, monocytes, lymphocytes (middle panels), but not to surface of blood debris including thrombocytes (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control Biotin antibody (MOPC-21) under same conditions (concentration in sample 5ug/ml, streptavidin APC, black-dashed histogram).)
Mouse anti-Human CD270 Antibody | anti-CD270 antibody
Anti-Hu CD270 Biotin
Gene Names
TNFRSF14; TR2; ATAR; HVEA; HVEM; CD270; LIGHTR
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry, Western Blot
Purity
Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
Synonyms
CD270; N/A; Anti-Hu CD270 Biotin; TNF receptor superfamily member 14; TNFRSF14, TR2, ATAR, HVEA, HVEM, LIGHTR; anti-CD270 antibody
Host
Mouse
Reactivity
Human
Isotype
Mouse IgG1 kappa
Clone Number
CW10
Specificity
The mouse monoclonal antibody CW10 recognizes an extracellular epitope on CD270, a type I transmembrane protein expressed on resting T cells, monocytes, and immature dendritic cells.
Purity/Purification
Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
Form/Format
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Applicable Applications for anti-CD270 antibody
IP (Immunoprecipitation), ELISA, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
recombinant human CD270
Conjugation
Biotin
Preparation and Storage
Store at 2-8 degree C. Do not freeze.
FCM/FACS (Flow Cytometry)
(Anti-Hu CD270 Biotin antibody (clone CW10) specificity verification by flow cytometry Anti-Hu CD270 Biotin antibody (concentration in sample 5ug/ml, streptavidin APC, red-filled histogram) binds specifically to CD270 on surface of SK-MEL-30 cells (upper panel) and also main leukocyte subpopulations - neutrophil granulocytes, monocytes, lymphocytes (middle panels), but not to surface of blood debris including thrombocytes (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control Biotin antibody (MOPC-21) under same conditions (concentration in sample 5ug/ml, streptavidin APC, black-dashed histogram).)
FCM/FACS (Flow Cytometry)
(Anti-Hu CD270 Biotin antibody (clone CW10) specificity verification by flow cytometry Anti-Hu CD270 Biotin antibody (concentration in sample 5ug/ml, streptavidin APC, red-filled histogram) binds specifically to CD270 on surface of SK-MEL-30 cells (upper panel) and also main leukocyte subpopulations - neutrophil granulocytes, monocytes, lymphocytes (middle panels), but not to surface of blood debris including thrombocytes (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control Biotin antibody (MOPC-21) under same conditions (concentration in sample 5ug/ml, streptavidin APC, black-dashed histogram).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD270 (CW10) Biotin antibody (concentration in sample 5ug/ml, Streptavidin APC).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD270 (CW10) Biotin antibody (concentration in sample 5ug/ml, Streptavidin APC).)
Related Product Information for anti-CD270 antibody
CD270 is a type I transmembrane protein of the TNFR superfamily, which is expressed on resting T cells, monocytes, and immature dendritic cells. Its ligands, CD258 and CD272, differ in effect on CD270 signaling. Whereas binding to CD258 provides a costimulatory signal, binding to CD272 gives to the cell an inhibitory signal. CD270 also is recognized by herpes simplex glycoprotein D. CD258-CD270 interaction and signaling is implicated in macrophage-derived foam cell-mediated development of atherosclerotic lesions.
References
*Whitbeck JC, Connolly SA, Willis SH, Hou W, Krummenacher C, de Leon MP, Lou H, Baribaud I, Eisenberg RJ, Cohen GH: Localization of the gD-binding region of the human herpes simplex virus receptor, HveA. J Virol. 2001 Jan;75(1):171-80., URL: https://pubmed.ncbi.nlm.nih.gov/11119586/,*Stiles KM, Whitbeck JC, Lou H, Cohen GH, Eisenberg RJ, Krummenacher C: Herpes simplex virus glycoprotein D interferes with binding of herpesvirus entry mediator to its ligands through downregulation and direct competition. J Virol. 2010 Nov;84(22):11646-60. , URL: https://pubmed.ncbi.nlm.nih.gov/20826693/,
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
283
NCBI Official Full Name
Tumor necrosis factor receptor superfamily member 14
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 14
NCBI Official Symbol
TNFRSF14
NCBI Official Synonym Symbols
TR2; ATAR; HVEA; HVEM; CD270; LIGHTR
NCBI Protein Information
tumor necrosis factor receptor superfamily member 14; CD40-like protein; herpes virus entry mediator A; tumor necrosis factor receptor-like gene2; tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator)
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 14
UniProt Gene Name
TNFRSF14
UniProt Synonym Gene Names
HVEA; HVEM; Herpesvirus entry mediator A; HveA; TR2
UniProt Entry Name
TNR14_HUMAN
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Product Notes
The CD270 tnfrsf14 (Catalog #AAA129367) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD270 Biotin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD270 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CD270 tnfrsf14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD270, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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