Mouse TCR Valpha24-Jalpha18 Antibody | anti-TCRValpha24-Jalpha18 antibody

Anti-Hu TCR Valpha24-Jalpha18 PE

Cat. Num. AAA129317

• Reactivity: Human, Non-human primates
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Synonyms: TCR Valpha24-Jalpha18; N/A; Anti-Hu TCR Valpha24-Jalpha18 PE; anti-TCRValpha24-Jalpha18 antibody

• Host: Mouse
• Reactivity: Human, Non-human primates
• Isotype: Mouse IgG1 kappa
• Clone Number: 6B11
• Specificity: The mouse monoclonal antibody 6B11 recognizes an extracellular epitope within V alpha 24 J alpha 18 TCR, which is expressed by invariant NK T cells (iNKT).
• Purity/Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• Applicable Applications for anti-TCRValpha24-Jalpha18 antibody: FCM/FACS (Flow Cytometry)
• Conjugation: PE
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human CD3 positive TCR Valpha24-Jalpha18 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human TCR Valpha24-Jalpha18 (6B11) PE antibody (10ul reagent/100ul of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry multicolor surface staining of human leukocytes stained using anti-human TCR Valpha24-Jalpha18 (6B11) PE antibody (10ul reagent/100ul of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10ul reagent/100ul of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR Valpha24-Jalpha18 (6B11) PE antibody (10ul reagent/100ul of peripheral whole blood).)

Related Product Information for anti-TCRValpha24-Jalpha18 antibody

TCR alpha V24-J18 chain is a marker of invariant NK T cells (iNKT). This chain pairs mostly with TCR beta V11 chain, and together they interact with glycolipide-loaded CD1d. This interaction results in iNKT activation and cytokine production (IFN gamma, IL-4, IL-13), which takes important part in immune regulation and host response to pathogens and cancer(support of both Th1 and Th2 way), although iNKT cells represent less than 1% of total peripheral T cells.

References

*Montoya CJ, Pollard D, Martinson J, Kumari K, Wasserfall C, Mulder CB, Rugeles MT, Atkinson MA, Landay AL, Wilson SB: Characterization of human invariant natural killer T subsets in health and disease using a novel invariant natural killer T cell-clonotypic monoclonal antibody, 6B11. Immunology. 2007 Sep;122(1):1-14., URL: https://pubmed.ncbi.nlm.nih.gov/17662044/,*Vyth-Dreese FA, Sein J, van de Kasteele W, Dellemijn TA, van den Bogaard C, Nooijen WJ, de Gast GC, Haanen JB, Bex A: Lack of anti-tumour reactivity despite enhanced numbers of circulating natural killer T cells in two patients with metastatic renal cell carcinoma. Clin Exp Immunol. 2010 Dec;162(3):447-59., URL: https://pubmed.ncbi.nlm.nih.gov/20942806/,*Krovi SH, Gapin L: Invariant natural killer T cell subsets - more than just developmental intermediates. Front Immunol. 2018 Jun 20;9:1393., URL: https://pubmed.ncbi.nlm.nih.gov/29973936/,*Chamoto K, Guo T, Scally SW, Kagoya Y, Anczurowski M, Wang CH, Rahman MA, Saso K, Butler MO, Chiu PP, Julien JP, Hirano N: Key residues at third CDR3beta position impact structure and antigen recognition of human invariant NK TCRs. J Immunol. 2017 Feb 1;198(3):1056-1065. , URL: https://pubmed.ncbi.nlm.nih.gov/28003379/,