CD16a (FcgR3a) Active Protein | CD16 active protein
Human CD16a Recombinant (FcgR3a)
Purity
>95%, as determined by SDS-PAGE and HPLC
Synonyms
CD16a (FcgR3a); N/A; Human CD16a Recombinant (FcgR3a); Human CD16a Recombinant (FcgrR3a) ; cd16; cd-16; fcgr3a; CD16 active protein
Host
N/A (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant CD16a is supplied as a 0.2 um filtered PBS solution, pH7.2.
Sequence Length
290
Domain
Ig-like C2
Source Note
Optimized DNA sequence encoding Human extracellular domain of human CD16a including a C-terminal His tag was expressed in HEK293 cells.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Biological Activity
Recombinant CD16a activity was measured by the ability to bind human IgG with an affinity constant of 180nM.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL.
Molecular Weight Note
Recombinant CD16a is a monomer protein consisting of 203 amino acid residue subunits, due to glycosylation migrates as an approximately 50 kDa protein on SDS-PAGE.
Preparation and Storage
Recombinant CD16a, as supplied, can be stored in working aliquots at 2-8°C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
Avoid repeated freeze/thaw cycles.
Related Product Information for CD16 active protein
Human CD16 is encoded by two genes, FcγRIIIA and FcγRIIIB. FcγRIIIa is a transmembranous isoform of the receptor expressed on the surface of natural killer (NK) cells, macrophages, a subset of monocytes and of T cells in association with dimers of the γ-chain of FcεRI and/or ζ-chain of T-cell receptors. FcγRIIIb is a glycosylphosphatidylinositol -anchored isoform restricted to neutrophils. FcγRIII plays a significant role in the clearance of immune complexes, antibody- dependent cellular cytotoxicity of K/NK cells,phagocytosis and antigen presentation.
Product Categories/Family for CD16 active protein
References
• affinity and kinetic analysis of fc receptor iiia (CD16a) binding to igg ligands .J. Biol. Chem., Mar 2007; 282: 6210 - 6221.
• phagocytosis stimulates mobilization and shedding of intracellular CD16a in human monocytes and macrophages: inhibition by hiv-1 infection .J. Leukoc. Biol., Feb 2006; 79: 294 - 302.
• phagocytosis stimulates mobilization and shedding of intracellular CD16a in human monocytes and macrophages: inhibition by hiv-1 infection .J. Leukoc. Biol., Feb 2006; 79: 294 - 302.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
low affinity immunoglobulin gamma Fc region receptor III-A isoform a
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor III-A
UniProt Gene Name
FCGR3A
UniProt Synonym Gene Names
CD16A; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa
UniProt Entry Name
FCG3A_HUMAN
Similar Products
Product Notes
The CD16 fcgr3a (Catalog #AAA14427) is an Active Protein produced from N/A (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders) and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CD16a (FcgR3a), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.