Human nucleophosmin (nucleolar phosphoprotein B23, numatrin) ELISA Kit | NPM1 elisa kit

Human Nucleophosmin, NPM1 ELISA Kit

Cat. Num. AAA18355

• Gene Names: NPM1; B23; NPM
• Reactivity: Human
• Synonyms: nucleophosmin (nucleolar phosphoprotein B23, numatrin); N/A; Human Nucleophosmin, NPM1 ELISA Kit; Human Nucleophosmin (NPM1) ELISA kit; B23; MGC104254; NPM; nucleophosmin 1; nucleophosmin/nucleoplasmin family; member 1; numatrin; nucleophosmin (nucleolar phosphoprotein B23; numatrin); NPM1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human NPM1. No significant cross-reactivity or interference between human NPM1 and analogues was observed.
• Sequence Length: 259
• Samples: Serum, plasma, tissue homogenates, cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 23.5 pg/ml-1500 pg/ml
• Sensitivity: < 5.8 pg/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for NPM1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NPM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NPM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NPM1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NPM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 83641870
• NCBI GeneID: 4869
• NCBI Accession #: NP_001032827.1
• NCBI GenBank Nucleotide #: NM_001037738.2
• UniProt Accession #: P06748; P08693; Q12826; Q13440; Q13441; Q14115; Q5EU94; Q5EU95; Q5EU96; A8K3N7; B5BU00; D3DQL6
• Molecular Weight: 32,575 Da
• NCBI Official Full Name: nucleophosmin isoform 3
• NCBI Official Synonym Full Names: nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• NCBI Official Symbol: NPM1
• NCBI Official Synonym Symbols: B23; NPM
• NCBI Protein Information: nucleophosmin; nucleolar protein NO38; nucleophosmin/nucleoplasmin family, member 1
• UniProt Protein Name: Nucleophosmin
• UniProt Gene Name: NPM1
• UniProt Synonym Gene Names: NPM; NPM
• UniProt Entry Name: NPM_HUMAN