Standard Curve (Sample)
Rat TRK A ELISA Kit | NTRK1 elisa kit
Rat TRK A ELISA Kit
Gene Names
Ntrk1; Trk
Reactivity
Rat
Applications
ELISA
Synonyms
TRK A; N/A; Rat TRK A ELISA Kit; MTC; TRK; TRKA; High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; TRK1-transforming tyrosine kinase protein; Tropomyosin-related kinase A; Tyrosine kinase receptor; Tyrosine kinase receptor A; Trk-A; gp140trk; p140-TrkA; NTRK1 elisa kit
Reactivity
Rat
Specificity
The Rat TRK A ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat TRK A proteins within the range of 78 pg/ml - 5000 pg/ml.
Sequence Length
799
Samples
Culture Supernatants, Cell Lysates, Tissue Homogenates
Sensitivity
< 40 pg/ml
Applicable Applications for NTRK1 elisa kit
ELISA (Sandwich ELISA)
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for NTRK1 elisa kit
Background: Tropomyosin receptor kinase A (TrkA) are efficacious in attenuating skeletal pain. TrkA mutants were able to activate signaling cascades and were even more efficient in promoting neurite outgrowth than the wild-type receptor. TrkA is part of a sub-family of protein kinases which includes TrkB and TrkC. Also, there are other neurotrophic factors structurally related to NGF: BDNF (for Brain-Derived Neurotrophic Factor), NT-3 (for Neurotrophin-3) and NT-4 (for Neurotrophin-4). While TrkA mediates the effects of NGF, TrkB is bound and activated by BDNF, NT-4, and NT-3. Further, TrkC binds and is activated by NT-3. TrkA receptor was found in kehumanoconus-affected corneas, along with an increased level of repressor isoform of Sp3 transcription factor.
Principle of the Assay: The Rat TRK A ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat TRK A in Cell Culture Supernatants, Cell Lysates, Tissue Homogenates. This assay employs an antibody specific for Rat TRK A coated on a 96-well plate. Standards and samples are pipetted into the wells and TRK A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat TRK A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TRK A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Principle of the Assay: The Rat TRK A ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat TRK A in Cell Culture Supernatants, Cell Lysates, Tissue Homogenates. This assay employs an antibody specific for Rat TRK A coated on a 96-well plate. Standards and samples are pipetted into the wells and TRK A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat TRK A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TRK A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
87,252 Da
NCBI Official Full Name
high affinity nerve growth factor receptor
NCBI Official Synonym Full Names
neurotrophic tyrosine kinase, receptor, type 1
NCBI Official Symbol
Ntrk1
NCBI Official Synonym Symbols
Trk
NCBI Protein Information
high affinity nerve growth factor receptor; trk; trk-A; p140-TrkA; TrkA neurotrophin receptor; trkA proto-oncogene receptor; tropomyosin-related kinase A; slow nerve growth factor receptor
UniProt Protein Name
High affinity nerve growth factor receptor
UniProt Gene Name
Ntrk1
UniProt Synonym Gene Names
Trk; Trka; Trk-A
UniProt Entry Name
NTRK1_RAT
Similar Products
Product Notes
The Rat NTRK1 ntrk1 (Catalog #AAA105770) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA105770 ELISA Kit recognizes Rat NTRK1. AAA Biotech's TRK A can be used in a range of immunoassay formats including, but not limited to, ELISA (Sandwich ELISA). Researchers should empirically determine the suitability of the NTRK1 ntrk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRK A, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.