Standard Curve (Sample)
(Rat TrkA PicoKine ELISA Kit standard curve)
Rat TrkA ELISA Kit | NTRK1 elisa kit
Rat TrkA PicoKine ELISA Kit
Gene Names
Ntrk1; Trk
Reactivity
Rat
Synonyms
TrkA; N/A; Rat TrkA PicoKine ELISA Kit; High affinity nerve growth factor receptor; neurotrophic tyrosine kinase, receptor, type 1; MTC; Neurotrophic tyrosine kinase receptor type 1; NTRK1; NTRK1_HUMAN; Oncogene TRK; p140-TrkA; TRK; Trk-A; TRK1; TRK1-transforming tyrosine kinase protein; NTRK1 elisa kit
Reactivity
Rat
Specificity
Natural and recombinant rat TrkA
Sequence Length
799
Samples
Cell culture supernates, Cell lysates and tissue homogenates
Assay Type
Sandwich
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
(Rat TrkA PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Rat TrkA PicoKine ELISA Kit standard curve)
Related Product Information for NTRK1 elisa kit
Principle of the assay: rat TrkA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TrkA has been precoated onto 96-well plates. Standards(NSO, A33-P418) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TrkA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat TrkA amount of sample captured in plate.
Background: Tropomyosin receptor kinase A (TrkA) are efficacious in attenuating skeletal pain.1 TrkA mutants were able toactivate signaling cascades and were even more efficient in promoting neurite outgrowth than the wild-type receptor.2 TrkA is part of a sub-family of protein kinases which includes TrkB and TrkC. Also, there are otherneurotrophic factors structurally related to NGF: BDNF (for Brain-Derived Neurotrophic Factor), NT-3 (for Neurotrophin-3) and NT-4 (for Neurotrophin-4). While TrkA mediates the effects of NGF, TrkB is bound and activated by BDNF, NT-4, and NT-3. Further, TrkC binds and is activated by NT-3.3 TrkA receptor was found inkeratoconus-affected corneas, along with an increased level of repressor isoform of Sp3 transcription factor.4 Thestandard product used in this kit is extracellular part (A33-P418) of recombinant rat TRKA, consisting of 386 amino acids with the molecular weight of 69KDa.
Background: Tropomyosin receptor kinase A (TrkA) are efficacious in attenuating skeletal pain.1 TrkA mutants were able toactivate signaling cascades and were even more efficient in promoting neurite outgrowth than the wild-type receptor.2 TrkA is part of a sub-family of protein kinases which includes TrkB and TrkC. Also, there are otherneurotrophic factors structurally related to NGF: BDNF (for Brain-Derived Neurotrophic Factor), NT-3 (for Neurotrophin-3) and NT-4 (for Neurotrophin-4). While TrkA mediates the effects of NGF, TrkB is bound and activated by BDNF, NT-4, and NT-3. Further, TrkC binds and is activated by NT-3.3 TrkA receptor was found inkeratoconus-affected corneas, along with an increased level of repressor isoform of Sp3 transcription factor.4 Thestandard product used in this kit is extracellular part (A33-P418) of recombinant rat TRKA, consisting of 386 amino acids with the molecular weight of 69KDa.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
87,252 Da
NCBI Official Full Name
High affinity nerve growth factor receptor
NCBI Official Synonym Full Names
neurotrophic tyrosine kinase, receptor, type 1
NCBI Official Symbol
Ntrk1
NCBI Official Synonym Symbols
Trk
NCBI Protein Information
high affinity nerve growth factor receptor; trk; trk-A; p140-TrkA; TrkA neurotrophin receptor; trkA proto-oncogene receptor; tropomyosin-related kinase A; slow nerve growth factor receptor
UniProt Protein Name
High affinity nerve growth factor receptor
UniProt Gene Name
Ntrk1
UniProt Synonym Gene Names
Trk; Trka; Trk-A
UniProt Entry Name
NTRK1_RAT
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Product Notes
The Rat NTRK1 ntrk1 (Catalog #AAA45339) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45339 ELISA Kit recognizes Rat NTRK1. It is sometimes possible for the material contained within the vial of "TrkA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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