Application Data
(HTRF assay for Recombinant NTRK1 (440-796, F589L) activity 1uM TK substrate was incubated with different concentrations of NTRK1 (440-796, F589L) protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100uM ATP for 1 hour. Then 10ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
NTRK1 recombinant protein
Recombinant NTRK1 (440-796, F589L) protein
Synonyms
NTRK1; N/A; Recombinant NTRK1 (440-796, F589L) protein; Neurotrophic Receptor Tyrosine Kinase 1; NTRK1 recombinant protein
Host
Baculovirus
Form/Format
Recombinant NTRK1 (440-796, F589L) protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20% glycerol, 0.04% Triton X-100, 0.5 mM TCEP.
Protein Species
Human
Protein Details
Recombinant NTRK1 (440-796, F589L) protein that includes amino acids 440-796 of human NTRK1 protein (accession number NP_002520.2) with an F589L mutation was expressed in a baculovirus expression system, and contains an N-terminal DYKDDDDK tag. The molecular weight of the protein is 41.32kDa.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Application Data
(HTRF assay for Recombinant NTRK1 (440-796, F589L) activity 1uM TK substrate was incubated with different concentrations of NTRK1 (440-796, F589L) protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100uM ATP for 1 hour. Then 10ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for Recombinant NTRK1 (440-796, F589L) activity 1uM TK substrate was incubated with different concentrations of NTRK1 (440-796, F589L) protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100uM ATP for 1 hour. Then 10ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE
(Recombinant NTRK1 (440-796, F589L)protein 10% SDS-PAGE gel stained with Coomassie Blue. MW: 41.32kDa Purity >90%)
SDS-PAGE
(Recombinant NTRK1 (440-796, F589L)protein 10% SDS-PAGE gel stained with Coomassie Blue. MW: 41.32kDa Purity >90%)
Related Product Information for NTRK1 recombinant protein
NTRK1 (Neurotrophic Receptor Tyrosine Kinase 1) is a receptor tyrosine kinase that involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. NTRK1 can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. High affinity receptor for NGF which is its primary ligand. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation (PubMed:1281417). Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation.
Product Categories/Family for NTRK1 recombinant protein
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Product Notes
The NTRK1 (Catalog #AAA60384) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "NTRK1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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