ELISA
(ELISA for PARP3 activity NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP3 in ADPR Buffer and incubated for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)
PARP-3 recombinant protein
Recombinant PARP-3 protein
Synonyms
PARP-3; N/A; Recombinant PARP-3 protein; Protein mono ADP ribosyltransferase PARP3; ADPRT3; ARTD3; IRT1; PARP-3 recombinant protein
Host
E coli
Form/Format
Recombinant PARP3 protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20% glycerol, 0.04% Triton X-100, 0.5 mM TCEP.
Protein Species
Human
Protein Details
Recombinant PARP3 protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_005476.4) with an N-terminal His and DYKDDDDK tag. The molecular weight of the protein is 64.6kDa.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
ELISA
(ELISA for PARP3 activity NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP3 in ADPR Buffer and incubated for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)
ELISA
(ELISA for PARP3 activity NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP3 in ADPR Buffer and incubated for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)
SDS-PAGE
(Recombinant PARP3 protein gel 10% SDS-PAGE Comassie staining MW: 64.6kDa Purity: >95%)
SDS-PAGE
(Recombinant PARP3 protein gel 10% SDS-PAGE Comassie staining MW: 64.6kDa Purity: >95%)
Related Product Information for PARP-3 recombinant protein
PARP3 (Protein mono-ADP-ribosyltransferase PARP3), also known as ADPRT3, ADPRTL2, ADPRTL3, ARTD3, IRT1 and PADPRT-3. PARP3 is a Mono-ADP-ribosyltransferase that mediates mono-ADP ribosylation of target proteins and plays a key role in the response to DNA damage. Mediates mono-ADP-ribosylation of glutamate, aspartate or lysine residues on target proteins. Involved in DNA repair by mediating mono-ADP-ribosylation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism, such as histone H2B, XRCC5 and XRCC6. Involved in single-strand break repair by catalyzing mono-ADP-ribosylation of histone H2B on ‘Glu-2’ (H2BE2ADPr) of nucleosomes containing nicked DNA.
Product Categories/Family for PARP-3 recombinant protein
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Product Notes
The PARP-3 (Catalog #AAA60410) is a Recombinant Protein produced from E coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PARP-3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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