PARP Recombinant Protein | PARP1 recombinant protein

PARP Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA257485

• Gene Names: Parp1; PARP; PPOL; ARTD1; Adprp; Adprt1; C80510; parp-1; sPARP-1; AI893648; 5830444G22Rik
• Purity: >85 % as determined by SDS-PAGE
• Synonyms: PARP; N/A; PARP Protein, Mouse, Recombinant (His Tag); Mouse PARP-1/PARP Protein (His Tag); poly (ADP-ribose) polymerase 1; 5830444G22Rik Protein, Mouse; Adprp Protein, Mouse; Adprt1 Protein, Mouse; AI893648 Protein, Mouse; ARTD1 Protein, Mouse; C80510 Protein, Mouse; PARP Protein, Mouse; parp-1 Protein, Mouse; PPOL Protein, Mouse; sPARP-1 Protein, Mouse; PARP1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH8.0, 10% gly, 0.1mM TCEP Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 1033aa
• Sequence: Met1-Trp1014

• Species: Mouse
• Tag: N-His
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Protein Construction: |A DNA sequence encoding the mouse PARP1 (NP_031441.2) (Met 1-Trp 1014) was fused with a polyhistidine tag at the N-terminus
• Activity: 1. Measured by its binding ability in a functional ELISA.; 2. Immobilized mouse PARP1 at 10ug/mL (100ul/well) can bind biotinylated human HSP70. The EC50 of biotinylated human HSP70 is 0.021ug/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Application Data

(Measured by its binding ability in a functional ELISA. Immobilized mouse PARP1 at 10 ug/mL (100 ul/well) can bind  biotinylated human HSP70, The EC50 of biotinylated human HSP70 is 0.021 ug/mL.)

Application Data

Related Product Information for PARP1 recombinant protein

Poly (ADP-ribose) polymerase 1(PRAP1), also known as NAD(+) ADP-ribosyltransferase 1(ADPRT), is a chromatin-associated enzyme that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 2-3 units. The poly(ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrated to mediate the poly(ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors are thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.

Product Categories/Family for PARP1 recombinant protein

Cancer Drug Targets

References

Malanga M. et al., 1998, J Biol Chem. 273: 11839-11843. Ariumi Y. et al., 1999, Oncogene. 18: 4616-4625. Helleday T. et al., 2005, Cell Cycle. 4: 1176-1178. Ahell I. et al., 2008, Nature. 451: 81-85.

NCBI and Uniprot Product Information

• NCBI GI #: 20806109
• NCBI GeneID: 11545
• NCBI Accession #: NP_031441.2
• NCBI GenBank Nucleotide #: NM_007415.2
• UniProt Accession #: Q921K2; P11103
• Molecular Weight: 112,721 Da
• NCBI Official Full Name: poly
• NCBI Official Synonym Full Names: poly (ADP-ribose) polymerase family, member 1
• NCBI Official Symbol: Parp1
• NCBI Official Synonym Symbols: PARP; PPOL; ARTD1; Adprp; Adprt1; C80510; parp-1; sPARP-1; AI893648; 5830444G22Rik
• NCBI Protein Information: poly [ADP-ribose] polymerase 1; poly [ADP-ribose] polymerase 1; msPARP; ADPRT 1; poly[ADP-ribose] synthase 1; poly[ADP-ribose] synthetase 1; NAD(+) ADP-ribosyltransferase 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase (NAD+, pol
• UniProt Protein Name: Poly (ADP-ribose) polymerase family, member 1
• UniProt Gene Name: Parp1
• UniProt Synonym Gene Names: Adprt1
• UniProt Entry Name: Q921K2_MOUSE