SDS-PAGE
(Purity: For specific information on a given lot, see related technical data sheet.)
PI3K (p110 delta (E1021K)/p85 alpha) Recombinant Protein | PI3K recombinant protein
PI3K (p110 delta (E1021K)/p85 alpha), Active
Applications
Western Blot
Synonyms
PI3K (p110 delta (E1021K)/p85 alpha); N/A; PI3K (p110 delta (E1021K)/p85 alpha), Active; p110 alpha: p110 delta; p85 alpha: PIK3R1; GRB1; p85-ALPHA; PI3K recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM sodium phosphate, pH7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Applicable Applications for PI3K recombinant protein
WB (Western Blot), Kinase Assay
Tag
HIS
Species
Human
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
(Purity: For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
Related Product Information for PI3K recombinant protein
Recombinant full-length human p110d (E1021K) and human p85a were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins. The p110d gene accession number is NM_005026; p85a is, NM_181523.
PI3K (p110 delta /p85 alpha) is a member of the PI3K family that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring (1). PI3K (p110delta/p85alpha) plays a role in oncogenic transformation and is consistently expressed at a high level in blast cells from AML. PI3K (p110delta/p85alpha) can play a selective role in the amplification of phosphatidylinositol (3,4,5) trisphosphate (PIP3) levels that lead to neutrophil polarization, and regulates directional migration, but not random locomotion (2). PI3K (p110delta/p85alpha) signaling pathway is involved in immune responses relevant to the pathogenesis of rheumatoid arthritis and other inflammatory diseases.
PI3K (p110 delta /p85 alpha) is a member of the PI3K family that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring (1). PI3K (p110delta/p85alpha) plays a role in oncogenic transformation and is consistently expressed at a high level in blast cells from AML. PI3K (p110delta/p85alpha) can play a selective role in the amplification of phosphatidylinositol (3,4,5) trisphosphate (PIP3) levels that lead to neutrophil polarization, and regulates directional migration, but not random locomotion (2). PI3K (p110delta/p85alpha) signaling pathway is involved in immune responses relevant to the pathogenesis of rheumatoid arthritis and other inflammatory diseases.
Product Categories/Family for PI3K recombinant protein
References
1. Vanhaesebroeck, B. et.al: p110-delta, a novel phosphoinositide 3-kinase in leukocytes. Proc. Nat. Acad. Sci. 94: 4330-4335, 1997.
2. Chantry, D. et.al: p110-delta, a novel phosphatidylinositol 3-kinase catalytic subunit that associates with p85 and is expressed predominantly in leukocytes. J. Biol. Chem. 272: 19236-19241,1997.
2. Chantry, D. et.al: p110-delta, a novel phosphatidylinositol 3-kinase catalytic subunit that associates with p85 and is expressed predominantly in leukocytes. J. Biol. Chem. 272: 19236-19241,1997.
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
P110 delta: 111kDa
p85 alpha: 86kDa
p85 alpha: 86kDa
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Product Notes
The PI3K (Catalog #AAA73811) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PI3K (p110 delta (E1021K)/p85 alpha) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Kinase Assay. Researchers should empirically determine the suitability of the PI3K for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI3K (p110 delta (E1021K)/p85 alpha), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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