Standard Curve (Sample)
(Mouse uPAR PicoKine ELISA Kit standard curve)
Mouse uPAR ELISA Kit | PLAUR elisa kit
Mouse uPAR PicoKine ELISA Kit
Gene Names
Plaur; Cd87; uPAR; u-PAR
Reactivity
Mouse
Synonyms
uPAR; N/A; Mouse uPAR PicoKine ELISA Kit; Urokinase plasminogen activator surface receptor; plasminogen activator, urokinase receptor; CD87; Monocyte activation antigen Mo3; PLAUR; U-PAR; UPAR_HUMAN; PLAUR elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse uPAR
Sequence Length
327
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
< 5pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
(Mouse uPAR PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Mouse uPAR PicoKine ELISA Kit standard curve)
Related Product Information for PLAUR elisa kit
Principle of the assay: mouse uPAR ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for uPAR has been precoated onto 96-well plates. Standards(NSO, L24-T297) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for uPAR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse uPAR amount of sample captured in plate.
Background: The urokinase-type plasminogen activator receptor (uPAR) is a key molecule in the regulation of cell-surface plasminogen activation and, as such, plays an important role in many normal as well as pathological processes.1 The cDNA for Mo3, an activation antigen expressed by human monocytes and myelomonocytic cell lines after stimulation by a variety of agents. Mo3 expression in vivo is associated predominantly with macrophages in inflammatory sites. It is a highly glycosylated protein of about 50 kD in monocytes where it is anchored to the plasma membrane by glycosyl-phosphatidylinositol linkage. The complete coding sequence of the cDNA has been found to encode 335 amino acids including a predicted signal peptide of 22 residues and a hydrophobic C-terminal portion. Mo3 is identical to the human receptor for the urokinase plasminogen activator.2 UPAR is a useful prognostic marker for biologically aggressive forms of endometrial cancer.3 PLAUR is located at chromosome 19q13.1-q13.2.1
Background: The urokinase-type plasminogen activator receptor (uPAR) is a key molecule in the regulation of cell-surface plasminogen activation and, as such, plays an important role in many normal as well as pathological processes.1 The cDNA for Mo3, an activation antigen expressed by human monocytes and myelomonocytic cell lines after stimulation by a variety of agents. Mo3 expression in vivo is associated predominantly with macrophages in inflammatory sites. It is a highly glycosylated protein of about 50 kD in monocytes where it is anchored to the plasma membrane by glycosyl-phosphatidylinositol linkage. The complete coding sequence of the cDNA has been found to encode 335 amino acids including a predicted signal peptide of 22 residues and a hydrophobic C-terminal portion. Mo3 is identical to the human receptor for the urokinase plasminogen activator.2 UPAR is a useful prognostic marker for biologically aggressive forms of endometrial cancer.3 PLAUR is located at chromosome 19q13.1-q13.2.1
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
24,619 Da
NCBI Official Full Name
Urokinase plasminogen activator surface receptor
NCBI Official Synonym Full Names
plasminogen activator, urokinase receptor
NCBI Official Symbol
Plaur
NCBI Official Synonym Symbols
Cd87; uPAR; u-PAR
NCBI Protein Information
urokinase plasminogen activator surface receptor; urokinase plasminogen activator receptor; urokinase-type plasminogen activator receptor
UniProt Protein Name
Urokinase plasminogen activator surface receptor
UniProt Gene Name
Plaur
UniProt Synonym Gene Names
U-PAR; uPAR
UniProt Entry Name
UPAR_MOUSE
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Product Notes
The Mouse PLAUR plaur (Catalog #AAA45865) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45865 ELISA Kit recognizes Mouse PLAUR. It is sometimes possible for the material contained within the vial of "uPAR, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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