SDS-PAGE
POLD1 recombinant protein
Recombinant Human POLD1 protein
Gene Names
POLD1; CDC2; POLD; CRCS10
Purity
>90% as determined by SDS-PAGE
Synonyms
POLD1; N/A; Recombinant Human POLD1 protein; DNA polymerase delta catalytic subunit; 3'-5' exodeoxyribonuclease; DNA polymerase subunit delta p125; POLD1 recombinant protein
Host
E Coli
Purity/Purification
>90% as determined by SDS-PAGE
Form/Format
Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA, 4% trehalose, 1% mannitol
Species
Homo sapiens (Human)
Protein Construction
A DNA sequence encoding the human POLD1 (Met41-Phe254) was fused with GST tag
Reconstitution
Reconstitute in sterile water for a stock solution.
Preparation and Storage
Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
SDS-PAGE
SDS-PAGE
Related Product Information for POLD1 recombinant protein
As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities. Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine, 8oxoG or abasic sites.
Product Categories/Family for POLD1 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
123,631 Da
NCBI Official Full Name
DNA polymerase delta catalytic subunit
NCBI Official Synonym Full Names
polymerase (DNA directed), delta 1, catalytic subunit
NCBI Official Symbol
POLD1
NCBI Official Synonym Symbols
CDC2; POLD; CRCS10
NCBI Protein Information
DNA polymerase delta catalytic subunit; CDC2 homolog; DNA polymerase subunit delta p125; polymerase (DNA directed), delta 1, catalytic subunit 125kDa
UniProt Protein Name
DNA polymerase delta catalytic subunit
UniProt Gene Name
POLD1
UniProt Synonym Gene Names
POLD
UniProt Entry Name
DPOD1_HUMAN
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Product Notes
The POLD1 pold1 (Catalog #AAA119947) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "POLD1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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