FCM/FACS (Flow Cytometry)
(POLD1 Antibody (Center) flow cytometry analysis of Jurkatcells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit POLD1 Polyclonal Antibody | anti-POLD1 antibody
POLD1 Antibody (Center)
Gene Names
POLD1; CDC2; MDPL; POLD; CRCS10
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
POLD1, Antibody; POLD1 Antibody (Center); DNA polymerase delta catalytic subunit; DNA polymerase subunit delta p125; POLD1; POLD; anti-POLD1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This POLD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 710-739 amino acids from the Central region of human POLD1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
710-739
Sequence Length
1,107
Applicable Applications for anti-POLD1 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(POLD1 Antibody (Center) flow cytometry analysis of Jurkatcells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(POLD1 Antibody (Center) flow cytometry analysis of Jurkatcells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of POLD1 Antibody (Center) in Jurkat cell line lysates (35ug/lane). POLD1 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of POLD1 Antibody (Center) in Jurkat cell line lysates (35ug/lane). POLD1 (arrow) was detected using the purified Pab.)
Related Product Information for anti-POLD1 antibody
The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA), the multisubunit replication factor C, and the 4 subunit polymerase complex: POLD1, POLD2, POLD3, and POLD4.
References
Chung D.W., Zhang J.,Proc. Natl. Acad. Sci. U.S.A. 88:11197-11201(1991)
Yang C.-L., Chang L.-S.Nucleic Acids Res. 20:735-745(1992)
Li H., Xie B., Zhou Y., Rahmeh A.J. Biol. Chem. 281:14748-14755(2006)
Yang C.-L., Chang L.-S.Nucleic Acids Res. 20:735-745(1992)
Li H., Xie B., Zhou Y., Rahmeh A.J. Biol. Chem. 281:14748-14755(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
123631
NCBI Official Full Name
DNA polymerase delta catalytic subunit 1
NCBI Official Synonym Full Names
polymerase (DNA directed), delta 1, catalytic subunit
NCBI Official Symbol
POLD1
NCBI Official Synonym Symbols
CDC2; MDPL; POLD; CRCS10
NCBI Protein Information
DNA polymerase delta catalytic subunit
UniProt Protein Name
DNA polymerase delta catalytic subunit
UniProt Gene Name
POLD1
UniProt Synonym Gene Names
POLD
UniProt Entry Name
DPOD1_HUMAN
Similar Products
Product Notes
The POLD1 pold1 (Catalog #AAA289956) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 710-739. The POLD1 Antibody (Center) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's POLD1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the POLD1 pold1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.