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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 8300-8350 of 10000 product results
WB (Western Blot)
(Western blot analysis of extracts from NIH/3T3 cells, using RGS5 antibody.)
RGS5, Polyclonal Antibody (Cat# AAA325068)
Full Name
RGS5 Antibody
Gene Names
RGS5; MST092; MST106; MST129; MSTP032; MSTP092; MSTP106; MSTP129
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Peptide affinity purification
Pricing
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells, using RPL7L1 antibody.)
RPL7L1, Polyclonal Antibody (Cat# AAA325090)
Full Name
RPL7L1 Antibody
Gene Names
RPL7L1; dJ475N16.4
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot)
(Western blot analysis of extracts from HepG2 using IL18BP antibody.)
IL18BP, Polyclonal Antibody (Cat# AAA325345)
Full Name
IL18BP Antibody
Gene Names
IL18BP; IL18BPa
Reactivity
Human, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IHC (Immunohistochemistry)
(AAA321433 at 1/100 staining rat gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Survivin, Polyclonal Antibody (Cat# AAA321433)
Full Name
Phospho-Survivin (Thr117) Antibody
Gene Names
BIRC5; API4; EPR-1
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-Abl (Tyr412) expression in various lysates)
Abl, Polyclonal Antibody (Cat# AAA321446)
Full Name
Phospho-Abl (Tyr412) Antibody
Gene Names
ABL1; ABL; JTK7; p150; c-ABL; v-abl; CHDSKM; c-ABL1; bcr/abl
Reactivity
Human, Mouse, Monkey
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Estrogen Receptor-alpha phosphorylation expression in 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Estrogen Receptor alpha, Polyclonal Antibody (Cat# AAA321457)
Full Name
Phospho-Estrogen Receptor alpha (Tyr537) Antibody
Gene Names
ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-C-RAF (Ser621) expression in various lysates)
C-RAF, Polyclonal Antibody (Cat# AAA321461)
Full Name
Phospho-C-RAF (Ser621) Antibody
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of PKC zeta phosphorylation expression in PMA treated NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
PKC zeta, Polyclonal Antibody (Cat# AAA321697)
Full Name
Phospho-PKC zeta (Thr410) Antibody
Gene Names
PRKCZ; PKC2; PKC-ZETA
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA321276 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Akt, Polyclonal Antibody (Cat# AAA321276)
Full Name
Phospho-Akt(Ser473) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(Colon tissue HIF1A antibody used at 1/200 on formalin-fixed paraffin embedded tissue.)
HIF1A, Polyclonal Antibody (Cat# AAA321279)
Full Name
HIF1A Antibody
Gene Names
HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot)
(Western blot analysis of extracts of C2C12 cells treated with TPA, using Phospho-CX43(Tyr265)Antibody.)
CX43, Polyclonal Antibody (Cat# AAA321290)
Full Name
Phospho-CX43(Tyr265) Antibody
Gene Names
GJA1; HSS; CMDR; CX43; EKVP; GJAL; ODDD; AVSD3; EKVP3; HLHS1; PPKCA
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA321135 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
AKAP14, Polyclonal Antibody (Cat# AAA321135)
Full Name
AKAP14 Antibody
Gene Names
AKAP14; AKAP28; PRKA14
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-PKC alpha (Thr638) expression in various lysates)
PKC alpha, Polyclonal Antibody (Cat# AAA321544)
Full Name
Phospho-PKC alpha (Thr638) Antibody
Gene Names
PRKCA; AAG6; PKCA; PRKACA; PKCI+/-; PKCalpha; PKC-alpha
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of extracts of MOLT-15 cells treated with phosphatase, using Phospho-LCP1 (Tyr28) Antibody.)
LCP1, Polyclonal Antibody (Cat# AAA321344)
Full Name
Phospho-LCP1 (Tyr28) Antibody
Gene Names
LCP1; LPL; CP64; PLS2; LC64P; HEL-S-37; L-PLASTIN
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis on NIH-3T3 cell lysate using ERD23 Antibody)
ERD23, Polyclonal Antibody (Cat# AAA321224)
Full Name
ERD23 Antibody
Gene Names
KDELR3; ERD2L3
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot)
(Western blot analysis of extracts from Mouse testis, using Cytokeratin 5 Ab at 1/1000 dilution.Observed bands:65kD.)
Keratin 5, Polyclonal Antibody (Cat# AAA320839)
Full Name
Keratin 5 Antibody
Gene Names
KRT5; K5; CK5; DDD; DDD1; EBS2; KRT5A
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IF (Immunofluorescence)
(AAA320899 staining HT29 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Gamma-synuclein, Polyclonal Antibody (Cat# AAA320899)
Full Name
Gamma-synuclein Antibody
Gene Names
SNCG; SR; BCSG1
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot)
(Western blot analysis of extracts from various samples, using NYREN18 Antibody. Lane 1: 293 treated with blocking peptide. Lane 2: 293; Lane 3: Hepg2;)
NYREN18, Polyclonal Antibody (Cat# AAA320913)
Full Name
NYREN18 Antibody
Gene Names
NUB1; BS4; NUB1L; NYREN18
Reactivity
Human, Mouse, Monkey
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot)
(Western blot analysis of extracts from 293 and Hela, using PIAS2 Antibody.)
PIAS2, Polyclonal Antibody (Cat# AAA320914)
Full Name
PIAS2 Antibody
Gene Names
PIAS2; DIP; MIZ1; SIZ2; ARIP3; PIASX; ZMIZ4
Reactivity
Human, Mouse, Rat, Monkey
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IHC (Immunohiostchemistry)
(AAA320760 at 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
GAB2, Polyclonal Antibody (Cat# AAA320760)
Full Name
GAB2 Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot)
(Western blot analysis of PDCD4 phosphorylation expression in serum treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
PDCD4, Polyclonal Antibody (Cat# AAA321753)
Full Name
Phospho-PDCD4 (Ser457) Antibody
Gene Names
PDCD4; H731
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA321776 staining MKN-45 cells treated with Forskolin 30 muM, 20 min by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
DARPP-32, Polyclonal Antibody (Cat# AAA321776)
Full Name
Phospho-DARPP-32 (Thr34) Antibody
Gene Names
PPP1R1B; DARPP32; DARPP-32
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA322174 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Amyloid beta A4, Polyclonal Antibody (Cat# AAA322174)
Full Name
Amyloid beta A4 Antibody
Gene Names
APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; preA4; CTFgamma
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IF (Immunofluorescence)
(AAA322190 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CDC2, Polyclonal Antibody (Cat# AAA322190)
Full Name
CDC2 Antibody
Gene Names
CDK1; CDC2; CDC28A; P34CDC2
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot)
(Western blot analysis of eIF4E expression in FBS treated NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
EIF4E, Polyclonal Antibody (Cat# AAA322192)
Full Name
EIF4E Antibody
Gene Names
EIF4E; CBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IHC (Immunohiostchemistry)
(AAA322231 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
ATF2, Polyclonal Antibody (Cat# AAA322231)
Full Name
ATF2 Antibody
Gene Names
ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1
Reactivity
Human, Mouse, Rat
Applications
Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IHC (Immunohistochemisry)
(AAA322267 at 1/100 staining Mouse intestine tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
CBL, Polyclonal Antibody (Cat# AAA322267)
Full Name
CBL Antibody
Gene Names
CBL; CBL2; NSLL; C-CBL; RNF55; FRA11B
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IHC (Immunohiostchemistry)
(AAA321861 at 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
BMP7, Polyclonal Antibody (Cat# AAA321861)
Full Name
BMP7 Antibody
Gene Names
BMP7; OP-1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot)
(Western blot analysis of PKM2 expression in HeLa cell lysate, The lane on the left is treated with the antigen-specific peptide.)
PKM2, Polyclonal Antibody (Cat# AAA321895)
Full Name
PKM2 Antibody
Gene Names
PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IF (Immunofluorescence)
(AAA322100 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
COX IV, Polyclonal Antibody (Cat# AAA322100)
Full Name
COX IV Antibody
Gene Names
COX4I1; COX4; COXIV; COX4-1; COXIV-1; COX IV-1
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IF (Immunofluorescence)
(AAA322134 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Aurora Kinase, Polyclonal Antibody (Cat# AAA322134)
Full Name
Aurora Kinase Antibody
Gene Names
AURKA; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; PPP1R47
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IHC (Immunohistochemisry)
(AAA321982 at 1/100 staining Rat colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
MMP 2, Polyclonal Antibody (Cat# AAA321982)
Full Name
MMP 2 Antibody
Gene Names
MMP2; CLG4; MONA; CLG4A; MMP-2; TBE-1; MMP-II
Reactivity
Human, Mouse, Rat, Monkey
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Peptide affinity purification
Pricing
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human epityphlon using AAA118962 at dilution 1:100)
Myotubularin, Polyclonal Antibody (Cat# AAA118962)
Full Name
Rabbit anti-human Myotubularin polyclonal Antibody(MTM1)
Gene Names
MTM1; CNM; MTMX; XLMTM
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Pricing
IHC (Immunohistochemisry)
(ALDH7A1 was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- ALDH7A1 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.)
ALDH7A1, Polyclonal Antibody (Cat# AAA46821)
Full Name
Anti-ALDH7A1 Antibody
Gene Names
ALDH7A1; EPD; PDE; ATQ1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of Gamma-tubulin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Gamma-tubulin, Polyclonal Antibody (Cat# AAA104846)
Full Name
Anti-Gamma-tubulin Antibody
Gene Names
TUBG1; TUBG; GCP-1; CDCBM4; TUBGCP1
Reactivity
Human, Monkey
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HER2 (Phospho-Tyr877) antibody (left) or the same antibody preincubated with with blocking peptide (right))
HER2, Polyclonal Antibody (Cat# AAA107624)
Full Name
HER2 antibody
Gene Names
ERBB2; HER2; erb2
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
HER2 antibody was purified by immunoaffinity chromatography.
Pricing
WB (Western Blot)
(Anti-RSK1 p90 antibody, AAA45384, All Western blottingAll lanes: Anti-RPS6KA1 (AAA45384) at 0.5ug/mlLane 1: A431 Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugPredicted bind size: 83KDObserved bind size: 83KD)
RSK1 p90, Polyclonal Antibody (Cat# AAA45384)
Full Name
Anti-RSK1 p90 antibody
Gene Names
RPS6KA1; RSK; HU-1; RSK1; MAPKAPK1A
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Pricing
Goat anti Monkey IgG (Fc specific), Polyclonal Secondary Antibody (Cat# AAA77609)
Full Name
Goat anti Monkey IgG (Fc specific)
Reactivity
Monkey
Applications
Immunoblot, Dot Blot, Immunofluorescence
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of DNAL1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
DNAL1, Polyclonal Antibody (Cat# AAA104541)
Full Name
Anti-DNAL1 Antibody
Gene Names
DNAL1; CILD16; C14orf168
Reactivity
Human, Mouse, Rat
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
retinal G protein coupled receptor, Polyclonal Antibody (Cat# AAA81198)
Full Name
Rabbit anti-human retinal G protein coupled receptor polyclonal Antibody
Gene Names
RGR; RP44
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Pricing
FCM/FACS (Flow Cytometry)
(ZNF28 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
ZNF28, Polyclonal Antibody (Cat# AAA283930)
Full Name
ZNF28 Antibody (N-term)
Gene Names
ZNF28; KOX24
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
FCM/FACS (Flow Cytometry)
(GSTK1 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
GSTK1, Polyclonal Antibody (Cat# AAA284200)
Full Name
GSTK1 Antibody (Center)
Gene Names
GSTK1; GST; GST13; hGSTK1; GSTK1-1; GST13-13; GST 13-13
Reactivity
Human, mouse
Applications
Immunohistochemistry, Western Blot, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Fluorescent confocal image of A2058 cell stained with PROX1 Antibody (C-term). A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PROX1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). PROX1 immunoreactivity is localized to Nucleus significantly.)
PROX1, Polyclonal Antibody (Cat# AAA284941)
Full Name
PROX1 Antibody (C-term)
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Immunofluorescence, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemisry)
(Anti-GPR45 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
GPR45, Polyclonal Antibody (Cat# AAA52290)
Full Name
Rabbit Polyclonal to Human GPR45
Gene Names
GPR45; PSP24; PSP24A; PSP24(ALPHA)
Reactivity
Gibbon, Human, Monkey, Rabbit
Predicted Reactivity: Gorilla, Bat (at least 90% immunogen sequence identity)
Predicted Reactivity: Gorilla, Bat (at least 90% immunogen sequence identity)
Applications
Immunohistochemistry
Purity
Immunoaffinity Purified
Pricing
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human esophagus using SERPINA1 antibody at dilution of 1:100 (40x lens).)
SERPINA1, Polyclonal Antibody (Cat# AAA37310)
Full Name
SERPINA1 Polyclonal Antibody
Gene Names
SERPINA1; PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using MAX antibody. Blue: DAPI for nuclear staining.)
MAX, Polyclonal Antibody (Cat# AAA37315)
Full Name
MAX Polyclonal Antibody
Gene Names
MAX; bHLHd4
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using ITGAM antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
ITGAM, Polyclonal Antibody (Cat# AAA37316)
Full Name
ITGAM Polyclonal Antibody
Gene Names
ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of extracts of HT-29 cells, using SSTR5 antibody (AAA37329) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
SSTR5, Polyclonal Antibody (Cat# AAA37329)
Full Name
SSTR5 Polyclonal Antibody
Gene Names
SSTR5; SS-5-R
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse kidney using CPT2 antibody at dilution of 1:100 (40x lens).)
CPT2, Polyclonal Antibody (Cat# AAA37337)
Full Name
CPT2 Polyclonal Antibody
Gene Names
CPT2; CPT1; IIAE4; CPTASE
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human colon using WISP2 Rabbit pAb (AAA37625) at dilution of 1:100 (40x lens).)
WISP2, Polyclonal Antibody (Cat# AAA37625)
Full Name
WISP2
Gene Names
WISP2; CCN5; CT58; CTGF-L
Applications
Immunohistochemistry, Western Blot
Purity
Affinity purification
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.