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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 9100-9150 of 96812 product results
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of RT4 cells using anti-PON2 antibody (AAA127093).Overlay histogram showing RT4 cells stained with AAA127093 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PON2 Antibody (AAA127093, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
PON2, Polyclonal Antibody (Cat# AAA127093)
Full Name
Anti-PON2 Antibody Picoband
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 4. IHC analysis of ATP1A2 using anti-ATP1A2 antibody (AAA127097).ATP1A2 was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ATP1A2 Antibody (AAA127097) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
ATP1A2, Polyclonal Antibody (Cat# AAA127097)
Full Name
Anti-ATP1A2 Antibody Picoband
Gene Names
ATP1A2; FHM2; MHP2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of JK cells using anti-PRPF19 antibody (AAA127117).Overlay histogram showing JK cells stained with AAA127117 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRPF19 Antibody (AAA127117, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PRPF19, Polyclonal Antibody (Cat# AAA127117)
Full Name
Anti-PRPF19 Antibody Picoband
Gene Names
PRPF19; PSO4; SNEV; PRP19; UBOX4; hPSO4; NMP200
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of SiHa cells using anti-Renin receptor/ATP6AP2 antibody (AAA127134).Overlay histogram showing SiHa cells stained with AAA127134 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Renin receptor/ATP6AP2 Antibody (AAA127134, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
Renin receptor/ATP6AP2, Polyclonal Antibody (Cat# AAA127134)
Full Name
Anti-Renin receptor/ATP6AP2 Antibody Picoband
Gene Names
ATP6AP2; M8-9; MRXE; XMRE; HT028; ELDF10; ATP6IP2; MSTP009; APT6M8-9; ATP6M8-9
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of A549 cells using anti-RAB1A antibody (AAA127137).Overlay histogram showing A549 cells stained with AAA127137 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB1A Antibody (AAA127137, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
RAB1A, Polyclonal Antibody (Cat# AAA127137)
Full Name
Anti-RAB1A Antibody Picoband
Gene Names
RAB1A; RAB1; YPT1
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of Klk7 using anti-Klk7 antibody (AAA127138).Klk7 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Klk7 Antibody (AAA127138) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
Klk7, Polyclonal Antibody (Cat# AAA127138)
Full Name
Anti-Klk7 Antibody Picoband
Gene Names
Klk1c7; Kal; KALA; Klk1; Klk5; Klk7; Klk1l; RGK-7; RSKG-7; RATKALA
Reactivity
Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-CDw75/ST6GAL1 antibody (AAA127165).Overlay histogram showing HepG2 cells stained with AAA127165 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDw75/ST6GAL1 Antibody (AAA127165, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
CDw75/ST6GAL1, Polyclonal Antibody (Cat# AAA127165)
Full Name
Anti-CDw75/ST6GAL1 Antibody Picoband
Gene Names
ST6GAL1; ST6N; SIAT1; ST6GalI
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of RT4 cells using anti-NR1H3 antibody (AAA127171).Overlay histogram showing RT4 cells stained with AAA127171 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NR1H3 Antibody (AAA127171, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NR1H3, Polyclonal Antibody (Cat# AAA127171)
Full Name
Anti-NR1H3 Antibody Picoband
Gene Names
NR1H3; LXRA; LXR-a; RLD-1
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 2. IF analysis of MNT using anti-MNT antibody (AAA127172) and anti-Beta Tubulin antibody (M01857-3).MNT was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-MNT Antibody (AAA127172) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
MNT, Polyclonal Antibody (Cat# AAA127172)
Full Name
Anti-MNT Antibody Picoband
Gene Names
MNT; ROX; MAD6; MXD6; bHLHd3
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of RAW264.7 cells using anti-Rpl9 antibody (AAA126628).Overlay histogram showing RAW264.7 cells stained with AAA126628 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Rpl9 Antibody (AAA126628, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rpl9, Polyclonal Antibody (Cat# AAA126628)
Full Name
Anti-Rpl9 Antibody Picoband
Reactivity
Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of PC-3 cells using anti-THUMPD3 antibody (AAA126642).Overlay histogram showing PC-3 cells stained with AAA126642 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-THUMPD3 Antibody (AAA126642, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
THUMPD3, Polyclonal Antibody (Cat# AAA126642)
Full Name
Anti-THUMPD3 Antibody Picoband
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of Daudi cells using anti-TIGD6 antibody (AAA126650).Overlay histogram showing Daudi cells stained with AAA126650 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TIGD6 Antibody (AAA126650, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TIGD6, Polyclonal Antibody (Cat# AAA126650)
Full Name
Anti-TIGD6 Antibody Picoband
Reactivity
Human, Rat
Applications
Western Blot, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U937 cells using anti-TOMM20L antibody (AAA126651).Overlay histogram showing U937 cells stained with AAA126651 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TOMM20L Antibody (AAA126651, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TOMM20L, Polyclonal Antibody (Cat# AAA126651)
Full Name
Anti-TOMM20L Antibody Picoband
Gene Names
TOMM20L; UNQ9438
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of RBM45 using anti-RBM45 antibody (AAA126670).RBM45 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- RBM45 (AAA126670) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
RBM45, Polyclonal Antibody (Cat# AAA126670)
Full Name
Anti-Human RBM45 Antibody
Reactivity
Human, Mouse
Applications
Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 5. IHC analysis of MECP2 using anti-MECP2 antibody (AAA126964).MECP2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MECP2 Antibody (AAA126964) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
MECP2, Polyclonal Antibody (Cat# AAA126964)
Full Name
Anti-MECP2 Antibody Picoband
Gene Names
MECP2; RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13
Reactivity
Human, Monkey, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of SiHa cells using anti-ATF4 antibody (AAA126984).Overlay histogram showing SiHa cells stained with AAA126984 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATF4 Antibody (AAA126984, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ATF4, Polyclonal Antibody (Cat# AAA126984)
Full Name
Anti-ATF4 Antibody Picoband
Gene Names
ATF4; CREB2; TXREB; CREB-2; TAXREB67
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HL-60 cells using anti-MPO antibody (AAA126985).Overlay histogram showing HL-60 cells stained with AAA126985 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MPO Antibody (AAA126985, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
MPO, Polyclonal Antibody (Cat# AAA126985)
Full Name
Anti-MPO Antibody Picoband
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 2. IF analysis of Galectin-3/LGALS3 using anti-Galectin-3/LGALS3 antibody (AAA127001).Galectin-3/LGALS3 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-Galectin-3/LGALS3 Antibody (AAA127001) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
LGALS3, Polyclonal Antibody (Cat# AAA127001)
Full Name
Anti-LGALS3 Antibody Picoband
Gene Names
LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of RH35 cells using anti-ROR Beta/RORB antibody (AAA126560).Overlay histogram showing RH35 cells stained with AAA126560 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ROR Beta/RORB Antibody (AAA126560, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ROR beta/RORB, Polyclonal Antibody (Cat# AAA126560)
Full Name
Anti-ROR beta/RORB Antibody Picoband
Gene Names
RORB; RZRB; NR1F2; ROR-BETA; RZR-BETA; bA133M9.1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of U87 cells using anti-ATP6V1A antibody (AAA126575).Overlay histogram showing U87 cells stained with AAA126575 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP6V1A Antibody (AAA126575, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ATP6V1A, Polyclonal Antibody (Cat# AAA126575)
Full Name
Anti-ATP6V1A Antibody Picoband
Gene Names
ATP6V1A; HO68; VA68; VPP2; Vma1; ATP6A1; ATP6V1A1
Reactivity
Human, Mouse, Rat, Monkey
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-TSPYL5 antibody (AAA126579).Overlay histogram showing MCF-7 cells stained with AAA126579 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TSPYL5 Antibody (AAA126579, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TSPYL5, Polyclonal Antibody (Cat# AAA126579)
Full Name
Anti-TSPYL5 Antibody Picoband
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of NRK cells using anti-SEPT5/SEPTIN5 antibody (AAA126590).Overlay histogram showing NRK cells stained with AAA126590 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEPT5/SEPTIN5 Antibody (AAA126590, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SEPT5/SEPTIN5, Polyclonal Antibody (Cat# AAA126590)
Full Name
Anti-SEPT5/SEPTIN5 Antibody Picoband
Gene Names
SEPT5; H5; CDCREL; PNUTL1; CDCREL1; CDCREL-1; HCDCREL-1
Reactivity
Human, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of SiHa cells using anti-ERLEC1 antibody (AAA126592).Overlay histogram showing SiHa cells stained with AAA126592 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ERLEC1 Antibody (AAA126592, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ERLEC1, Polyclonal Antibody (Cat# AAA126592)
Full Name
Anti-ERLEC1 Antibody Picoband
Gene Names
ERLEC1; CIM; XTP3-B; C2orf30; CL24936; CL25084; XTP3TPB
Reactivity
Human
Applications
Western Blot, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 2. IF analysis of GCC1 using anti-GCC1 antibody (AAA126605).GCC1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-GCC1 Antibody (AAA126605) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
GCC1, Polyclonal Antibody (Cat# AAA126605)
Full Name
Anti-GCC1 Antibody Picoband
Gene Names
GCC1; GCC1P; GCC88
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of JK cells using anti-UBAP2 antibody (AAA126608).Overlay histogram showing JK cells stained with AAA126608 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBAP2 Antibody (AAA126608, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
UBAP2, Polyclonal Antibody (Cat# AAA126608)
Full Name
Anti-UBAP2 Antibody Picoband
Gene Names
UBAP2; UBAP-2
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of HeLa cells using anti-MRPS18B antibody (AAA126611).Overlay histogram showing HeLa cells stained with AAA126611 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MRPS18B Antibody (AAA126611, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MRPS18B, Polyclonal Antibody (Cat# AAA126611)
Full Name
Anti-MRPS18B Antibody Picoband
Gene Names
MRPS18B; PTD017; S18amt; C6orf14; HSPC183; MRPS18-2; HumanS18a; MRP-S18-2
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HL-60 cells using anti-WDR12 antibody (AAA126612).Overlay histogram showing HL-60 cells stained with AAA126612 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WDR12 Antibody (AAA126612, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WDR46, Polyclonal Antibody (Cat# AAA126612)
Full Name
Anti-WDR46 Antibody Picoband
Gene Names
WDR46; UTP7; BING4; FP221; C6orf11
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of RH35 cells using anti-TMEM65 antibody (AAA126621).Overlay histogram showing RH35 cells stained with AAA126621 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM65 Antibody (AAA126621, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TMEM65, Polyclonal Antibody (Cat# AAA126621)
Full Name
Anti-TMEM65 Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of RT4 cells using anti-RHNO1 antibody (AAA126622).Overlay histogram showing RT4 cells stained with AAA126622 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RHNO1 Antibody (AAA126622, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
RHNO1, Polyclonal Antibody (Cat# AAA126622)
Full Name
Anti-RHNO1 Antibody Picoband
Gene Names
RHNO1; RHINO; C12orf32; HKMT1188
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of A549 cells using anti-APC1/ANAPC1 antibody (AAA127180).Overlay histogram showing A549 cells stained with AAA127180 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APC1/ANAPC1 Antibody (AAA127180, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
APC1/ANAPC1, Polyclonal Antibody (Cat# AAA127180)
Full Name
Anti-APC1/ANAPC1 Antibody Picoband
Gene Names
ANAPC1; APC1; MCPR; TSG24
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IP (Immunoprecipitation)
(Figure 3. Immunoprecipitating ARID3A in HepG2 whole cell lysate .Western blot analysis of ARID3A using anti-ARID3A antibody (AAA127189).Lane 1: HepG2 whole cell lysates (30ug),Lane 2: Rabbit control IgG instead of anti-ARID3A antibody in HepG2 whole cell lysate,Lane 3: anti-ARID3A antibody (2ug) + HepG2 whole cell lysate (500ug).After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ARID3A antigen affinity purified polyclonal antibody (AAA127189) at a dilution of 0.5ug/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Light chain). The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for ARID3A at approximately 80 kDa. The expected band size for ARID3A is at 63 kDa.)
ARID3A, Polyclonal Antibody (Cat# AAA127189)
Full Name
Anti-ARID3A Antibody Picoband
Gene Names
ARID3A; DRIL1; DRIL3; BRIGHT; E2FBP1
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 4. IHC analysis of Renin/REN using anti-Renin/REN antibody (AAA127194).Renin/REN was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Renin/REN Antibody (AAA127194) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
Renin/REN, Polyclonal Antibody (Cat# AAA127194)
Full Name
Anti-Renin/REN Antibody Picoband
Gene Names
REN; HNFJ2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of HepG2 cells using anti-RPS14 antibody (AAA127195).Overlay histogram showing HepG2 cells stained with AAA127195 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS14 Antibody (AAA127195, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
RPS14, Polyclonal Antibody (Cat# AAA127195)
Full Name
Anti-RPS14 Antibody Picoband
Gene Names
RPS14; S14; EMTB
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of JK cells using anti-3BP2/SH3BP2 antibody (AAA127209).Overlay histogram showing JK cells stained with AAA127209 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-3BP2/SH3BP2 Antibody (AAA127209, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
3BP2/SH3BP2, Polyclonal Antibody (Cat# AAA127209)
Full Name
Anti-3BP2/SH3BP2 Antibody Picoband
Gene Names
SH3BP2; 3BP2; CRBM; CRPM; 3BP-2; RES4-23
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of JK cells using anti-MELK antibody (AAA127211).Overlay histogram showing JK cells stained with AAA127211 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MELK Antibody (AAA127211, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MELK, Polyclonal Antibody (Cat# AAA127211)
Full Name
Anti-MELK Antibody Picoband
Gene Names
MELK; HPK38
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HEL cells using anti-SH2D3C antibody (AAA127234).Overlay histogram showing HEL cells stained with AAA127234 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SH2D3C Antibody (AAA127234, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SH2D3C, Polyclonal Antibody (Cat# AAA127234)
Full Name
Anti-SH2D3C Antibody Picoband
Gene Names
SH2D3C; CHAT; NSP3; SHEP1; PRO34088
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of SH-SY5Y cells using anti-MPST antibody (AAA127241).Overlay histogram showing SH-SY5Y cells stained with AAA127241 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MPST Antibody (AAA127241, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MPST, Polyclonal Antibody (Cat# AAA127241)
Full Name
Anti-MPST Antibody Picoband
Gene Names
MPST; MST; TST2; TUM1
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-ANKRD1 antibody (AAA127245).Overlay histogram showing HepG2 cells stained with AAA127245 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ANKRD1 Antibody (AAA127245, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
ANKRD1, Polyclonal Antibody (Cat# AAA127245)
Full Name
Anti-ANKRD1 Antibody Picoband
Gene Names
ANKRD1; ALRP; CARP; C-193; CVARP; MCARP; bA320F15.2
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of MGAT4B using anti-MGAT4B antibody (AAA127648).MGAT4B was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MGAT4B Antibody (AAA127648) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
MGAT4B, Polyclonal Antibody (Cat# AAA127648)
Full Name
Anti-MGAT4B Antibody Picoband
Gene Names
MGAT4B; GNT-IV; GNT-IVB
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U937 cells using anti-OXER1 antibody (AAA127651).Overlay histogram showing U937 cells stained with AAA127651 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-OXER1 Antibody (AAA127651, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
OXER1, Polyclonal Antibody (Cat# AAA127651)
Full Name
Anti-OXER1 Antibody Picoband
Gene Names
OXER1; GPCR; GPR170; TG1019
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of 293T cells using anti-NARF antibody (AAA127659).Overlay histogram showing 293T cells stained with AAA127659 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NARF Antibody (AAA127659, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NARF, Polyclonal Antibody (Cat# AAA127659)
Full Name
Anti-NARF Antibody Picoband
Gene Names
NARF; IOP2
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 2. IF analysis of MED29 using anti-MED29 antibody (AAA127665) and anti-Beta Tubulin antibody (M01857-3).MED29 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-MED29 Antibody (AAA127665) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
MED29, Polyclonal Antibody (Cat# AAA127665)
Full Name
Anti-MED29 Antibody Picoband
Gene Names
MED29; IXL
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of Hela cells using anti-NUP54 antibody (AAA127678).Overlay histogram showing Hela cells stained with AAA127678 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUP54 Antibody (AAA127678, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NUP54, Polyclonal Antibody (Cat# AAA127678)
Full Name
Anti-NUP54 Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of JK cells using anti-MAN2C1 antibody (AAA127682).Overlay histogram showing JK cells stained with AAA127682 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAN2C1 Antibody (AAA127682, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MAN2C1, Polyclonal Antibody (Cat# AAA127682)
Full Name
Anti-MAN2C1 Antibody Picoband
Gene Names
MAN2C1; MANA; MANA1; MAN6A8
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-PLXNC1 antibody (AAA127684).Overlay histogram showing HepG2 cells stained with AAA127684 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PLXNC1 Antibody (AAA127684, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PLXNC1, Polyclonal Antibody (Cat# AAA127684)
Full Name
Anti-PLXNC1 Antibody Picoband
Gene Names
PLXNC1; CD232; VESPR; PLXN-C1
Reactivity
Human
Applications
Western Blot, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-INTS12 antibody (AAA127709).Overlay histogram showing MCF-7 cells stained with AAA127709 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-INTS12 Antibody (AAA127709, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
INTS12, Polyclonal Antibody (Cat# AAA127709)
Full Name
Anti-INTS12 Antibody Picoband
Gene Names
INTS12; INT12; PHF22; SBBI22
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 4. IF analysis of ITPRIP using anti-ITPRIP antibody (AAA127714).ITPRIP was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-ITPRIP Antibody (AAA127714) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
ITPRIP, Polyclonal Antibody (Cat# AAA127714)
Full Name
Anti-ITPRIP Antibody Picoband
Gene Names
ITPRIP; DANGER; KIAA1754; bA127L20; bA127L20.2
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of HL-60 cells using anti-RPN2 antibody (AAA127272).Overlay histogram showing HL-60 cells stained with AAA127272 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPN2 Antibody (AAA127272, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
RPN2, Polyclonal Antibody (Cat# AAA127272)
Full Name
Anti-RPN2 Antibody Picoband
Gene Names
RPN2; SWP1; RPNII; RIBIIR; RPN-II
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of HEL cells using anti-OLAH antibody (AAA127274).Overlay histogram showing HEL cells stained with AAA127274 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OLAH Antibody (AAA127274, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
OLAH, Polyclonal Antibody (Cat# AAA127274)
Full Name
Anti-OLAH Antibody Picoband
Gene Names
OLAH; SAST; AURA1; THEDC1
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of SiHa cells using anti-STRADB antibody (AAA127282).Overlay histogram showing SiHa cells stained with AAA127282 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STRADB Antibody (AAA127282, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
STRADB, Polyclonal Antibody (Cat# AAA127282)
Full Name
Anti-STRADB Antibody Picoband
Gene Names
STRADB; PAPK; ILPIP; ILPIPA; ALS2CR2; CALS-21; PRO1038
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. This ability to bind multiple epitopes is what makes polyclonal antibodies highly sensitive, as explained in our detailed guide on polyclonal antibodies and why they matter.
As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in our catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.