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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 4800-4850 of 118597 product results
IF (Immunofluorescence)
(Immunofluorescence staining of PDAP1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PDAP1 polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Cell membrane.)
PDAP1, Polyclonal Antibody (Cat# AAA259529)
Full Name
Anti-PDAP1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
WB (Western Blot)
(Western blot analysis of LTBP1 expression in HEK293T (A), Raw264.7 (B), H9C2 (C) whole cell lysates.)
LTBP1, Polyclonal Antibody (Cat# AAA259539)
Full Name
Anti-LTBP1 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of Connexin 37 expression in HEK293T (A), Hela (B) whole cell lysates.)
Connexin 37, Polyclonal Antibody (Cat# AAA259541)
Full Name
Anti-Connexin 37 Antibody
Gene Names
GJA4; CX37
Reactivity
Human, Mouse, Rat, Bovine, Porcine, Rabbit, Sheep
Applications
Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of Histone Deacetylase 8 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Histone Deacetylase 8, Polyclonal Antibody (Cat# AAA259571)
Full Name
Anti-Histone Deacetylase 8 Antibody
Gene Names
HDAC8; HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1
Reactivity
Human, Mouse, Rat, Monkey
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of AKT staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
AKT, Polyclonal Antibody (Cat# AAA259583)
Full Name
Anti-AKT Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Zebrafish
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of ITPKC staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
ITPKC, Polyclonal Antibody (Cat# AAA259592)
Full Name
Anti-ITPKC Antibody
Gene Names
ITPKC; IP3KC; IP3-3KC
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IP (Immunoprecipitation)
(CCAR1 was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate Lane B:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-CCAR1 rabbit polyclonal antibody and 60ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CCAR1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 132 kDa Observed band size :150 kDa)
CCAR1, Polyclonal Antibody (Cat# AAA259453)
Full Name
Anti-CCAR1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of MRPS5 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
MRPS5, Polyclonal Antibody (Cat# AAA259622)
Full Name
Anti-MRPS5 Antibody
Gene Names
MRPS5; S5mt; MRP-S5
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Kanadaptin staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Kanadaptin, Polyclonal Antibody (Cat# AAA259641)
Full Name
Anti-Kanadaptin Antibody
Gene Names
SLC4A1AP; HLC3
Reactivity
Human, Mouse, Rat, Monkey
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of SMAD3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
SMAD3, Polyclonal Antibody (Cat# AAA259644)
Full Name
Anti-SMAD3 Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse, Rat, Chicken, Monkey, Porcine, Sheep
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of MRPL21 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
MRPL21, Polyclonal Antibody (Cat# AAA259811)
Full Name
Anti-MRPL21 Antibody
Gene Names
MRPL21; L21mt; MRP-L21
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of SENP6 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
SENP6, Polyclonal Antibody (Cat# AAA259817)
Full Name
Anti-SENP6 Antibody
Gene Names
SENP6; SSP1; SUSP1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of Cathepsin F expression in HEK293T (A), Raw264.7 (B), H9C2 (C) whole cell lysates.)
Cathepsin F, Polyclonal Antibody (Cat# AAA259821)
Full Name
Anti-Cathepsin F Antibody
Gene Names
CTSF; CATSF; CLN13
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of MSH3 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
MSH3, Polyclonal Antibody (Cat# AAA260272)
Full Name
Anti-MSH3 Antibody
Gene Names
MSH3; DUP; MRP1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of IL-10 expression in Romas (A), HeLa (B), mouse liver (C) whole cell lysates.)
IL-10, Polyclonal Antibody (Cat# AAA260280)
Full Name
Anti-IL-10 Antibody
Gene Names
IL10; CSIF; TGIF; GVHDS; IL-10; IL10A
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Beta-tubulin staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Beta-tubulin, Polyclonal Antibody (Cat# AAA260283)
Full Name
Anti-Beta-tubulin Antibody
Gene Names
TUBB3; CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of 14-3-3 theta staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
14-3-3 theta, Polyclonal Antibody (Cat# AAA260062)
Full Name
Anti-14-3-3 theta Antibody
Gene Names
YWHAQ; 1C5; HS1; 14-3-3
Reactivity
Human, Mouse, Rat, Bovine, Monkey
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of CHD2 expression in HEK293T (A), A549 (B) whole cell lysates.)
CHD2, Polyclonal Antibody (Cat# AAA260068)
Full Name
Anti-CHD2 Antibody
Gene Names
CHD2; EEOC
Reactivity
Human, Rat, Monkey
Applications
Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of CstF-64T staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
CstF-64T, Polyclonal Antibody (Cat# AAA260530)
Full Name
Anti-CstF-64T Antibody
Gene Names
CSTF2T; CstF-64T
Reactivity
Human, Mouse, Rat, Bovine
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of GAS8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
GAS8, Polyclonal Antibody (Cat# AAA259848)
Full Name
Anti-GAS8 Antibody
Gene Names
GAS8; GAS11
Reactivity
Human, Mouse, Rat, Bovine
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of CEP110 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
CEP110, Polyclonal Antibody (Cat# AAA259893)
Full Name
Anti-CEP110 Antibody
Gene Names
CCP110; CP110; Cep110
Reactivity
Human, Monkey
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of FATP5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
FATP5, Polyclonal Antibody (Cat# AAA259908)
Full Name
Anti-FATP5 Antibody
Gene Names
SLC27A5; BAL; ACSB; BACS; FATP5; ACSVL6; FACVL3; FATP-5; VLACSR; VLCSH2; VLCS-H2
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of c-Myc staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
c-Myc, Polyclonal Antibody (Cat# AAA260472)
Full Name
Anti-c-Myc Antibody
Gene Names
MYC; MRTL; MYCC; c-Myc; bHLHe39
Reactivity
Human, Mouse, Rat, Bovine, Dog, Sheep
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of BDKRB1 staining in Saos2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
BDKRB1, Polyclonal Antibody (Cat# AAA260476)
Full Name
Anti-BDKRB1 Antibody
Gene Names
BDKRB1; B1R; BKR1; B1BKR; BKB1R; BDKRB2; BRADYB1
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of SMIT1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
SMIT1, Polyclonal Antibody (Cat# AAA259945)
Full Name
Anti-SMIT1 Antibody
Gene Names
SLC5A3; BCW2; SMIT; SMIT1; SMIT2
Reactivity
Human, Rat, Bovine, Dog, Pig, Rabbit
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of GNG13 expression in HeLa (A), Raw264.7 (B), H9C2 (C) whole cell lysates.)
GNG13, Polyclonal Antibody (Cat# AAA259986)
Full Name
Anti-GNG13 Antibody
Gene Names
GNG13; h2-35; G(gamma)13
Reactivity
Human, Mouse, Rat, Bovine, Monkey
Applications
Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of ACOT4 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
ACOT4, Polyclonal Antibody (Cat# AAA259995)
Full Name
Anti-ACOT4 Antibody
Gene Names
ACOT4; PTE1B; PTE2B; PTE-Ib
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Histone H2B (AcK15) staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Histone H2B, Polyclonal Antibody (Cat# AAA260160)
Full Name
Anti-Histone H2B (AcK15) Acetyl Antibody
Reactivity
Human, Mouse, Rat, Bovine, Dog, Monkey
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of DUSP6 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
DUSP6, Polyclonal Antibody (Cat# AAA260222)
Full Name
Anti-DUSP6 Antibody
Gene Names
DUSP6; HH19; MKP3; PYST1
Reactivity
Human, Mouse, Rat, Bovine, Dog, Porcine
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of TRK B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
TRK B, Polyclonal Antibody (Cat# AAA260994)
Full Name
Anti-TRK B Antibody
Gene Names
NTRK2; TRKB; trk-B; GP145-TrkB
Reactivity
Human, Mouse, Rat, Chicken, Monkey, Pig
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of S100-A1 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
S100-A1, Polyclonal Antibody (Cat# AAA261046)
Full Name
Anti-S100-A1 Antibody
Gene Names
S100A1; S100; S100A; S100-alpha
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of PU.1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
PU.1, Polyclonal Antibody (Cat# AAA261050)
Full Name
Anti-PU.1 Antibody
Gene Names
SPI1; OF; PU.1; SFPI1; SPI-1; SPI-A
Reactivity
Human, Mouse, Rat, Chicken, Pig, Zebrafish
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of p53 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
p53, Polyclonal Antibody (Cat# AAA261139)
Full Name
Anti-p53 Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of QSK staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
QSK, Polyclonal Antibody (Cat# AAA260567)
Full Name
Anti-QSK Antibody
Gene Names
SIK3; L19; QSK; SIK-3
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of CaMKK beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
CaMKK beta, Polyclonal Antibody (Cat# AAA260568)
Full Name
Anti-CaMKK beta Antibody
Gene Names
CAMKK2; CAMKK; CAMKKB
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of CD32b staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
CD32b, Polyclonal Antibody (Cat# AAA260912)
Full Name
Anti-CD32b Antibody
Gene Names
FCGR2B; CD32; FCG2; CD32B; FCGR2; IGFR2
Reactivity
Human, Mouse, Rat, Monkey
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of LRRC41 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
LRRC41, Polyclonal Antibody (Cat# AAA260701)
Full Name
Anti-LRRC41 Antibody
Gene Names
LRRC41; MUF1; PP7759
Reactivity
Human, Mouse, Rat, Bovine
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of RPL5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
RPL5, Polyclonal Antibody (Cat# AAA260703)
Full Name
Anti-RPL5 Antibody
Gene Names
RPL5; L5; DBA6; MSTP030; PPP1R135
Reactivity
Human, Mouse, Rat, Chicken, Monkey, Zebrafish
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of Histone H2B (AcK12) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Histone H2B, Polyclonal Antibody (Cat# AAA260740)
Full Name
Anti-Histone H2B (AcK12) Acetyl Antibody
Gene Names
HIST1H2BA; STBP; TH2B; H2BFU; TSH2B; TSH2B.1; bA317E16.3
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of GAPDH staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
GAPDH, Polyclonal Antibody (Cat# AAA260755)
Full Name
Anti-GAPDH Antibody
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human, Mouse, Rat, Rabbit, Chicken, Monkey, Sheep
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of UBR1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
UBR1, Polyclonal Antibody (Cat# AAA260891)
Full Name
Anti-UBR1 Antibody
Gene Names
UBR1; JBS
Reactivity
Human, Mouse, Rat, Monkey
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of PITX1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
PITX1, Polyclonal Antibody (Cat# AAA260830)
Full Name
Anti-PITX1 Antibody
Gene Names
PITX1; BFT; CCF; POTX; PTX1; LBNBG
Reactivity
Human, Mouse, Rat, Chicken
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of DUSP9 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
DUSP9, Polyclonal Antibody (Cat# AAA221047)
Full Name
Rabbit anti-DUSP9 Antibody
Gene Names
DUSP9; MKP4; MKP-4
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of BCL2 (pT56) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
BCL2, Polyclonal Antibody (Cat# AAA221052)
Full Name
Rabbit anti-BCL2 (pT56) Antibody
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human, Rat
Applications
Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of GPR170 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
GPR170, Polyclonal Antibody (Cat# AAA221056)
Full Name
Rabbit anti-GPR170 Antibody
Gene Names
OXER1; GPCR; GPR170; TG1019
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of PDK4 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
PDK4, Polyclonal Antibody (Cat# AAA221062)
Full Name
Rabbit anti-PDK4 Antibody
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of 15-LO2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
15-LO2, Polyclonal Antibody (Cat# AAA221067)
Full Name
Rabbit anti-15-LO2 Antibody
Gene Names
ALOX15B; 15-LOX-2
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of LLGL2 staining in human prostate formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
LLGL2, Polyclonal Antibody (Cat# AAA220785)
Full Name
Rabbit anti-LLGL2 Antibody
Gene Names
LLGL2; HGL; LGL2
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of CENP-C staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
CENP-C, Polyclonal Antibody (Cat# AAA220798)
Full Name
Rabbit anti-CENP-C Antibody
Gene Names
CENPC; MIF2; hcp-4; CENP-C; CENPC1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Granzyme B staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Granzyme B, Polyclonal Antibody (Cat# AAA220802)
Full Name
Rabbit anti-Granzyme B Antibody
Gene Names
GZMB; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
Reactivity
Human, Mouse
Applications
Immunochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. This ability to bind multiple epitopes is what makes polyclonal antibodies highly sensitive, as explained in our detailed guide on polyclonal antibodies and why they matter.
As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in our catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.