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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 4750-4800 of 10000 product results
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U251 cells using anti-TRAT1 antibody (AAA126591).Overlay histogram showing U251 cells stained with AAA126591 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAT1 Antibody (AAA126591, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TRAT1, Polyclonal Antibody (Cat# AAA126591)
Full Name
Anti-TRAT1 Antibody Picoband
Gene Names
TRAT1; TRIM; TCRIM; HSPC062
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemisry)
(DAB staining on IHC-P;Samples: Human Liver Tissue;Primary Ab: 20ug/ml Rabbit Anti-Human COQ10B AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: ))
Coenzyme Q10 Homolog B, Polyclonal Antibody (Cat# AAA142825)
Full Name
Polyclonal Antibody to Coenzyme Q10 Homolog B (COQ10B)
Reactivity
Human, Rat, Pig
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
IF (Immunofluorescence)
(AAA322567 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Neurexin I, Polyclonal Antibody (Cat# AAA322567)
Full Name
Neurexin I Antibody
Gene Names
NRXN1; PTHSL2; SCZD17; Hs.22998
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IF (Immunofluorescence)
(AAA326233 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(DF6465) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).)
TPH1, Polyclonal Antibody (Cat# AAA326233)
Full Name
TPH1 Antibody
Gene Names
TPH1; TPRH; TRPH
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Peptide affinity purification
Pricing
WB (Western Blot)
(Western blot analysis of extracts of MGC-803, using CTSE antibody. The lane on the left is treated with the antigen-specific peptide.)
CTSE, Polyclonal Antibody (Cat# AAA326802)
Full Name
CTSE Antibody
Gene Names
CTSE; CATE
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
Application Data
(Anti-VEGF Picoband antibody, AAA45665-3.jpgAll lanes: Anti-VEGF(AAA45665) at 0.5ug/mlLane 1: Rat Thymus Tissue Lysate at 40ugLane 2: Rat Brain Tissue Lysate at 40ugPredicted bind size: 27KDObserved bind size: 27KD)
VEGF, Polyclonal Antibody (Cat# AAA45665)
Full Name
Anti-VEGF Antibody
Gene Names
VEGFA; VPF; VEGF; MVCD1
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of DBF4 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
DBF4, Polyclonal Antibody (Cat# AAA105352)
Full Name
Anti-DBF4 Antibody
Gene Names
DBF4; ASK; CHIF; DBF4A; ZDBF1
Reactivity
Human, Bovine, Pig
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using AAA240157(PIWIL2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))
PIWIL2, Polyclonal Antibody (Cat# AAA240157)
Full Name
PIWIL2 Antibody
Gene Names
PIWIL2; CT80; HILI; mili; PIWIL1L
Reactivity
Human
Applications
Immunohistochemistry
Purity
Antigen Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed Hela cells using JunB(Ab-79) Antibody.)
JUNB, Polyclonal Antibody (Cat# AAA242067)
Full Name
JUNB (Ab-79) Antibody
Gene Names
JUNB; AP-1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were Purified by affinity-chromatography using epitope-specific peptide.
Pricing
SDS-PAGE
(Gel: 8%SDS-PAGE,Lysate: 40 ug,,Primary antibody: AAA241712(SGPL1 Antibody) at dilution 1/200 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 1 second)
SGPL1, Polyclonal Antibody (Cat# AAA241712)
Full Name
SGPL1 Antibody
Gene Names
SGPL1; SPL; S1PL
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purification
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of THP-1 cells using anti-GPCR TGR5/GPBAR1 antibody (AAA126191).Overlay histogram showing THP-1 cells stained with AAA126191 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GPCR TGR5/GPBAR1 Antibody (AAA126191, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
GPCR TGR5/GPBAR1, Polyclonal Antibody (Cat# AAA126191)
Full Name
Anti-GPCR TGR5/GPBAR1 Antibody Picoband
Gene Names
GPBAR1; BG37; TGR5; M-BAR; GPCR19; GPR131
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded Mouse spinal cord using AXIN2 Polyclonal Antibody at dilution of 1:100 (40x lens).)
AXIN2, Polyclonal Antibody (Cat# AAA177593)
Full Name
AXIN2 Polyclonal Antibody
Gene Names
AXIN2; AXIL; ODCRCS
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Purity
Affinity purification
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of U20S cells using anti-TMOD2 antibody (AAA127762).Overlay histogram showing U20S cells stained with AAA127762 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMOD2 Antibody (AAA127762, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
TMOD2, Polyclonal Antibody (Cat# AAA127762)
Full Name
Anti-TMOD2 Antibody Picoband
Gene Names
TMOD2; NTMOD; N-TMOD
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohiostchemistry)
(AAA327213 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
Catalase, Polyclonal Antibody (Cat# AAA327213)
Full Name
Catalase Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IHC (Immunohiostchemistry)
(AAA326390 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
ALDH1A1, Polyclonal Antibody (Cat# AAA326390)
Full Name
ALDH1A1 Antibody
Gene Names
ALDH1A1; ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IHC (Immunohistochemisry)
(AAA327422 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
STXBP1, Polyclonal Antibody (Cat# AAA327422)
Full Name
STXBP1 Antibody
Gene Names
STXBP1; P67; NSEC1; UNC18; RBSEC1; MUNC18-1
Reactivity
Human, Mouse, Monkey
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded Human colon cancer using MMP28 Polyclonal Antibody at dilution of 1:20)
MMP28, Polyclonal Antibody (Cat# AAA166614)
Full Name
MMP28 Polyclonal Antibody
Gene Names
MMP28; MM28; MMP25; MMP-25; MMP-28; EPILYSIN
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antigen affinity purification
Pricing
IHC (Immunohistochemisry)
(DAB staining on IHC-P. Samples: Human Tissue))
Somatostatin (SST), Polyclonal Antibody (Cat# AAA131647)
Full Name
Polyclonal Antibody to Somatostatin (SST)
Gene Names
SST; SMST
Reactivity
Human, Mouse, Rat, Pig
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Affinity Chromatography
Pricing
IHC (Immunohiostchemistry)
(DAB staining on IHC-P; Samples: Human Pancreas Tissue))
CUB And Zona Pellucida Like Domains Protein 1 (CUZD1), Polyclonal Antibody (Cat# AAA133570)
Full Name
Polyclonal Antibody to CUB And Zona Pellucida Like Domains Protein 1 (CUZD1)
Gene Names
CUZD1; ERG1; ERG-1; UO-44; ITMAP1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Affinity Chromatography
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using DEFA5 Polyclonal Antibody at dilution 1:15)
DEFA5, Polyclonal Antibody (Cat# AAA166249)
Full Name
DEFA5 Polyclonal Antibody
Gene Names
DEFA5; DEF5; HD-5
Reactivity
Human
Applications
Immunohistochemistry
Purity
Affinity purification
Pricing
WB (Western Blot)
(Western blot analysis of extracts of Mouse lung using PRKCSH Polyclonal Antibody at 1:1000 dilution.)
PRKCSH, Polyclonal Antibody (Cat# AAA179403)
Full Name
PRKCSH Polyclonal Antibody
Gene Names
PRKCSH; PCLD; PLD1; G19P1; PKCSH; AGE-R2
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity purification
Pricing
Application Data
USP29, Polyclonal Antibody (Cat# AAA303403)
Full Name
USP29 Antibody
Gene Names
USP29; HOM-TES-84/86
Applications
Immunohistochemistry, Western Blot
Purity
Affinity purified
Pricing
WB (Western Blot)
(Western blot analysis of K562 293T using FAT10 antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000)
FAT10, Polyclonal Antibody (Cat# AAA318703)
Full Name
FAT10 Rabbit Polyclonal Antibody
Gene Names
UBD; FAT10; UBD-3; GABBR1
Reactivity
Human, Rat, Mouse
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
WB (Western Blot)
(Western Blot analysis of Hela cells using primary antibody diluted at 1:1000(4 degree C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25 degree C, 1 hour).)
PCNA, Polyclonal Antibody (Cat# AAA319927)
Full Name
PCNA Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
IP (Immunoprecipitation)
(MAP2K2 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-MAP2K2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-MAP2K2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 44 kDaObserved band size :47 kDa)
MEK2, Polyclonal Antibody (Cat# AAA256582)
Full Name
Anti-MEK2 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IHC (Immunohiostchemistry)
(IHC image of AAA243495 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
ATF5, Polyclonal Antibody (Cat# AAA243495)
Full Name
ATF5 Antibody
Gene Names
ATF5; ATFX; HMFN0395
Reactivity
Human
Applications
Immunohistochemistry
Purity
Antigen Affinity Purified
Pricing
WB (Western Blot)
(Western blot analysis of extracts of rat brain tissue, using CPLX1 antibody.)
CPLX1, Polyclonal Antibody (Cat# AAA323016)
Full Name
CPLX1 Antibody
Gene Names
CPLX1; CPX1; CPX-I; EIEE63
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot)
(Western blot analysis of extracts from A549 cells, using CDH7 antibody.The lane on the left is treated with the antigen-specific peptide.)
CDH7, Polyclonal Antibody (Cat# AAA324221)
Full Name
CDH7 Antibody
Gene Names
CDH7; CDH7L1
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot)
(Western blot analysis of Hela whole cell lysates, using ACTN1 Antibody. The lane on the left is treated with the antigen-specific peptide.)
ACTN1, Polyclonal Antibody (Cat# AAA326073)
Full Name
ACTN1 Antibody
Gene Names
ACTN1; BDPLT15
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IF (Immunofluorescence)
(AAA326680 staining HuvEc cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
TOLLIP, Polyclonal Antibody (Cat# AAA326680)
Full Name
TOLLIP Antibody
Gene Names
TOLLIP; IL-1RAcPIP
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded Human liver cancer tissue using SGPL1 Polyclonal Antibody at dilution 1:40)
SGPL1, Polyclonal Antibody (Cat# AAA167310)
Full Name
SGPL1 Polyclonal Antibody
Gene Names
SGPL1; SPL; S1PL
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Purity
Antigen affinity purification
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded Human liver cancer using NDUFA9 Polyclonal Antibody at dilution of 1:20)
NDUFA9, Polyclonal Antibody (Cat# AAA167703)
Full Name
NDUFA9 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antigen affinity purification
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded Human brain using BMP4 Polyclonal Antibody at dilution of 1:25)
BMP4, Polyclonal Antibody (Cat# AAA169369)
Full Name
BMP4 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antigen affinity purification
Pricing
WB (Western Blot)
(WB Suggested Anti-C11orf24 Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2 cell lysateC11orf24 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells)
C11orf24, Polyclonal Antibody (Cat# AAA200027)
Full Name
C11orf24 antibody - N-terminal region
Gene Names
C11orf24; DM4E3
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded Human breast cancer tissue using SPARCL1 Polyclonal Antibody at dilution 1:30)
SPARCL1, Polyclonal Antibody (Cat# AAA170550)
Full Name
SPARCL1 Polyclonal Antibody
Gene Names
SPARCL1; SC1; MAST9; PIG33; MAST 9
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Purity
Affinity purification
Pricing
WB (Western Blot)
(Western Blot analysis of 3T3 cells with PAK5 Polyclonal Antibody.)
PAK5, Polyclonal Antibody (Cat# AAA172138)
Full Name
PAK5 Polyclonal Antibody
Gene Names
PAK7; PAK5
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Pricing
WB (Western Blot)
(Western Blot analysis of Mouse heart tissue using ENTPD1 Polyclonal Antibody at dilution of 1/200)
ENTPD1, Polyclonal Antibody (Cat# AAA173350)
Full Name
ENTPD1 Polyclonal Antibody
Gene Names
ENTPD1; CD39; SPG64; ATPDase; NTPDase-1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purification
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded mouse lung using ESR2 antibody at dilution of 1:200 (40x lens).)
ESR2, Polyclonal Antibody (Cat# AAA37331)
Full Name
ESR2 Polyclonal Antibody
Gene Names
ESR2; Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of JK cells using anti-MIS12 antibody (AAA127408).Overlay histogram showing JK cells stained with AAA127408 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MIS12 Antibody (AAA127408, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MIS12, Polyclonal Antibody (Cat# AAA127408)
Full Name
Anti-MIS12 Antibody Picoband
Gene Names
MIS12; MTW1; hMis12; KNTC2AP; 2510025F08Rik
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western blot analysis of extracts of K562, using CDC25C antibody. The lane on the left is treated with the antigen-specific peptide.)
CDC25C, Polyclonal Antibody (Cat# AAA326312)
Full Name
CDC25C Antibody
Gene Names
CDC25C; CDC25; PPP1R60
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IF (Immunofluorescence)
(AAA327106 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
TACR1, Polyclonal Antibody (Cat# AAA327106)
Full Name
TACR1 Antibody
Gene Names
TACR1; SPR; NK1R; NKIR; TAC1R
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IHC (Immunohistochemisry)
(VDAC2 Antibody-Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE) HIER using 10 mM sodium citrate buffer pH 6.0)
VDAC2, Polyclonal Antibody (Cat# AAA163314)
Full Name
VDAC2 Rabbit anti-Human Polyclonal (C-terminal domain of human) Antibody
Gene Names
VDAC2; POR
Reactivity
Human
Applications
Immunohistochemistry
Purity
Immunoaffinity purified
Pricing
IHC (Immunohiostchemistry)
(NR0B1/DAX1 Antibody-Anti-DAX1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.)
NR0B1/DAX1, Polyclonal Antibody (Cat# AAA162484)
Full Name
NR0B1/DAX1 Rabbit anti-Human Polyclonal (aa359-408) Antibody
Gene Names
NR0B1; AHC; AHX; DSS; GTD; HHG; AHCH; DAX1; DAX-1; NROB1; SRXY2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
Immunoaffinity purified
Pricing
WB (Western Blot)
(Western blot analysis of Anti-PEX11B Antibody at a 1:500 dilution. Lane 1: MCF-7 cell lysate. Lane 2: Raw264.7 cell lysate. Lane 3: PC12 cell lysate.)
PEX11B, Polyclonal Antibody (Cat# AAA162169)
Full Name
Anti-PEX11B Antibody (aa132-177) IHC-plus
Gene Names
PEX11B; PEX14B; PEX11-BETA
Reactivity
Mouse, Rat, Human
Applications
Immunoprecipitation, Western Blot, Immunocytochemistry, Immunohistochemistry
Purity
Immunoaffinity purified
Pricing
IF (Immunofluorescence)
(Detection of H2AK9ac by immunofluorescence. U2OS cells were stained with H2AK9ac antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: H2AK9ac antibody staining. Right panel: merge.)
Histone H2AK9ac, Polyclonal Antibody (Cat# AAA59828)
Full Name
Histone H2AK9ac antibody (pAb)
Gene Names
HIST2H2AC; H2A; H2A/q; H2AFQ; H2A-GL101
Reactivity
Human, Wide Range Predicted
Applications
Western Blot, Immunofluorescence, Dot Blot
Pricing
IHC (Immunohiostchemistry)
(IHC image of AAA243536 diluted at 1:300 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
HSPD1, Polyclonal Antibody (Cat# AAA243536)
Full Name
HSPD1 Antibody
Gene Names
HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
>95%, Protein G Purified
Pricing
IHC (Immunohiostchemistry)
(The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using AAA240145(PAX9 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200))
PAX9, Polyclonal Antibody (Cat# AAA240145)
Full Name
PAX9 Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Purity
Antigen Affinity Purification
Pricing
SDS-PAGE
(Gel: 8%SDS-PAGE lysate40 ¦Œg, Lane 1-4: LoVo and A549 cell Mouseliver tissue and Rat liver tissue lysatesPrimary antibody: 46511£¨CLCN7 Antibody) at dilution 1/300Secondary antibody: Goat anti rabbit IgG at 1/8000 dilutionExposure time: 20 seconds)
CLCN7, Polyclonal Antibody (Cat# AAA310373)
Full Name
CLCN7 Antibody
Gene Names
CLCN7; CLC7; CLC-7; OPTA2; OPTB4; PPP1R63
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Antigen affinity purification
Pricing
IHC (Immunohiostchemistry)
(The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using (FOXF2 Antibody) at dilution 1/100. (Original magnification: -200)
FOXF2, Polyclonal Antibody (Cat# AAA308029)
Full Name
FOXF2 Antibody
Gene Names
FOXF2; FKHL6; FREAC2; FREAC-2
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Antigen affinity purification.
Pricing
SIRPA, Polyclonal Antibody (Cat# AAA312748)
Full Name
SIRPA Polyclonal Conjugated Antibody
Gene Names
SIRPA; BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1
Reactivity
Human, Mouse, Rat
Purity
Affinity purification
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.