Rabbit anti-Human, Rat 53BP1 Polyclonal Antibody | anti-53BP1 antibody

Phospho-53BP1 (Ser1618) Antibody

Cat. Num. AAA329473

• Gene Names: TP53BP1; p202; 53BP1
• Reactivity: Human, Rat
• Applications: ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: 53BP1, Antibody; Phospho-53BP1 (Ser1618) Antibody; 53 BP1; 53BP1; FLJ41424; MGC138366; p202; p53 binding protein 1; p53 BP1; p53-binding protein 1; p53BP1; TP53 BP1; TP53B_HUMAN; Tp53bp1; TRP53 BP1; Tumor protein 53 binding protein 1; Tumor protein p53 binding protein 1; Tumor suppressor p53 binding protein 1; Tumor suppressor p53-binding protein 1; anti-53BP1 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Phospho-53BP1 (Ser1618) Antibody detects endogenous levels of 53BP1 only when phosphorylated at Ser1618.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Supplied in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-53BP1 antibody: ELISA (Peptide ELISA), ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human 53BP1 around the phosphorylation site of Ser1618.
• Preparation and Storage: Store at -20°C. Stable for 12 months from date of receipt.

Application Data

(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistochemisry)

(At 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohiostchemistry)

(Staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis of extracts from HeLa thymidine nocodazole, using Phospho-53BP1 (Ser1618) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 213972634
• NCBI GeneID: 7158
• NCBI Accession #: NP_001135451.1
• NCBI GenBank Nucleotide #: NM_001141979.1
• UniProt Accession #: Q12888; Q2M1Z7; Q4LE46; Q5FWZ3; Q7Z3U4
• Molecular Weight: 450kDa
• NCBI Official Full Name: tumor suppressor p53-binding protein 1 isoform 2
• NCBI Official Synonym Full Names: tumor protein p53 binding protein 1
• NCBI Official Symbol: TP53BP1
• NCBI Official Synonym Symbols: p202; 53BP1
• NCBI Protein Information: tumor suppressor p53-binding protein 1; p53BP1; tumor protein 53-binding protein, 1; tumor protein p53-binding protein, 1
• UniProt Protein Name: Tumor suppressor p53-binding protein 1
• UniProt Gene Name: TP53BP1
• UniProt Synonym Gene Names: 53BP1; p53-binding protein 1; p53BP1
• UniProt Entry Name: TP53B_HUMAN