WB (Western Blot)
(Western blot analysis using acid sphingomyelinase antibody on normal human brain lysate (7 ug/lane). Antibody used at 1 ug/ml (1) and 0.5 ug/ml (2) and detected using mouse anti-rabbit antibody at 1:75k dilution and visualized using Pierce West Femto substrate.)
Rabbit anti-Human Acid Sphingomyelinase Polyclonal Antibody | anti-SMPD1 antibody
Acid Sphingomyelinase
Gene Names
SMPD1; ASM; NPD; ASMASE
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Ammonium Sulfate Precipitation
Synonyms
Acid Sphingomyelinase, Antibody; Acid Sphingomyelinase; aSMase; SMPD1; ASM; Sphingomyelin phosphodiesterase; ASM-1; anti-SMPD1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Ammonium Sulfate Precipitation
Form/Format
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Sequence Length
631
Applicable Applications for anti-SMPD1 antibody
ELISA, WB (Western Blot)
Immunogen
Synthetic peptide derived from human acid sphingomyelinase protein.
Preparation and Storage
Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles
WB (Western Blot)
(Western blot analysis using acid sphingomyelinase antibody on normal human brain lysate (7 ug/lane). Antibody used at 1 ug/ml (1) and 0.5 ug/ml (2) and detected using mouse anti-rabbit antibody at 1:75k dilution and visualized using Pierce West Femto substrate.)
WB (Western Blot)
(Western blot analysis using acid sphingomyelinase antibody on normal human brain lysate (7 ug/lane). Antibody used at 1 ug/ml (1) and 0.5 ug/ml (2) and detected using mouse anti-rabbit antibody at 1:75k dilution and visualized using Pierce West Femto substrate.)
Related Product Information for anti-SMPD1 antibody
Human acid sphingomyelinase (sphingomyelin phosphodiesterase, ASM) is the lysosomal enzyme responsible for the hydrolysis of sphingomyelin to ceramide and phosphocholine. Converts sphingomyelin to ceramide. aSM also has phospholipase C activities toward 1,2-diacylglycerol-phosphocholine and 1,2-diacylglycerol-phosphoglycerol. The enzyme is a membrane-associated glycoprotein with a pH optimum of about 4.5 and a subunit molecular mass of about 72 kDa. In addition AtoS M, two other sphingomyelinases have been identified in man, a Mg2+- dependent neutral sphingomyelinase found primarily in brain and a Zn2+-dependent acid sphingomyelinase found primarily in serum. Although it is likely that the acid and neutral sphingomyelinases are coded by different genes, the molecular genetic relationship of these three biochemically distinct sphingomyelinases has not been determined. Understanding the role of these sphingomyelinases in the hydrolysis of sphingomyelin to ceramide will be an important step in the understanding of ceramide as it is further hydrolyzed to sphingosine, a neutral phospholipid which has been implicated in the regulation of protein kinase C-mediated signal transduction. Inherited deficiencies of ASM have been reported in man, deficient ASM activity results in the two major subtypes of Niemann-Pick disease (NPD).
Product Categories/Family for anti-SMPD1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
sphingomyelin phosphodiesterase isoform 1
NCBI Official Synonym Full Names
sphingomyelin phosphodiesterase 1, acid lysosomal
NCBI Official Symbol
SMPD1
NCBI Official Synonym Symbols
ASM; NPD; ASMASE
NCBI Protein Information
sphingomyelin phosphodiesterase; acid sphingomyelinase
UniProt Protein Name
Sphingomyelin phosphodiesterase
UniProt Gene Name
SMPD1
UniProt Synonym Gene Names
ASM; aSMase
UniProt Entry Name
ASM_HUMAN
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Product Notes
The SMPD1 smpd1 (Catalog #AAA60506) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Acid Sphingomyelinase reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Acid Sphingomyelinase can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SMPD1 smpd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Acid Sphingomyelinase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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