WB (Western Blot)
(Detection of human ACL by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ACL antibody (AAA212332 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse ACL Polyclonal Antibody | anti-ACL antibody
Rabbit anti-ACL Antibody, Affinity Purified
Gene Names
ACLY; ACL; ATPCL; CLATP
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ACL, Antibody; Rabbit anti-ACL Antibody, Affinity Purified; ATPCL; CLATP; citrate cleavage enzyme; ATP-citrate synthase; ACL; ATP-citrate (pro-S-)-lyase; ATP citrate lyase; anti-ACL antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ACL antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Sheep, Bovine
Immunogen
Between 375 and 425
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human ACL by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ACL antibody (AAA212332 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human ACL by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ACL antibody (AAA212332 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human ACL by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, Hep-G2, HEK293T, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ACL antibody AAA212332 (AAA212332 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human ACL by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, Hep-G2, HEK293T, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ACL antibody AAA212332 (AAA212332 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human ACL by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ACL antibody (AAA212332 lot 2) used for IP at 6 ug per reaction. ACL was also immunoprecipitated by a previous lot of this antibody (AAA212332 lot 1) and a second antibody against a different epitope of ACL . For blotting immunoprecipitated ACL, AAA212332 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human ACL by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ACL antibody (AAA212332 lot 2) used for IP at 6 ug per reaction. ACL was also immunoprecipitated by a previous lot of this antibody (AAA212332 lot 1) and a second antibody against a different epitope of ACL . For blotting immunoprecipitated ACL, AAA212332 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Related Product Information for anti-ACL antibody
ATP citrate lyase (ACL) is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. ACL is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ACL may be involved in the biosynthesis of acetylcholine [taken from NCBI Entrez Gene (Gene ID: 47)].
Product Categories/Family for anti-ACL antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58kD
NCBI Official Full Name
ATP-citrate synthase isoform 2
NCBI Official Synonym Full Names
ATP citrate lyase
NCBI Official Symbol
ACLY
NCBI Official Synonym Symbols
ACL; ATPCL; CLATP
NCBI Protein Information
ATP-citrate synthase; ATP citrate synthase; citrate cleavage enzyme; ATP-citrate (pro-S-)-lyase
UniProt Protein Name
ATP-citrate synthase
UniProt Gene Name
ACLY
UniProt Synonym Gene Names
ACL
UniProt Entry Name
ACLY_HUMAN
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Product Notes
The ACL acly (Catalog #AAA212332) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ACL Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ACL can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ACL acly for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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