FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of hela cells using ACTR3 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit ACTR3 Polyclonal Antibody | anti-ACTR3 antibody
ACTR3 Antibody (C-term)
Gene Names
ACTR3; ARP3
Reactivity
Human (Predicted Reactivity: Bovine, Chicken, Mouse, Rat)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ACTR3, Antibody; ACTR3 Antibody (C-term); Actin-related protein 3; Actin-like protein 3; ACTR3; ARP3; anti-ACTR3 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Chicken, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This ACTR3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-407 amino acids from the C-terminal region of human ACTR3.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
380-407
Sequence Length
418
Applicable Applications for anti-ACTR3 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9000076). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9000076). Seems to contact the pointed end of the daughter actin filament (PubMed:9000076). In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation (PubMed:29058690). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:17220302, PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947). Plays a role in ciliogenesis (PubMed:20393563).
Cellular Location
Cytoplasm, cytoskeleton. Cell projection. Nucleus. Note=In pre- apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes) (PubMed:19109554)
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of hela cells using ACTR3 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of hela cells using ACTR3 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ACTR3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ACTR3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of ACTR3 Antibody (C-term) in A2058 cell line lysates (35ug/lane). ACTR3 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of ACTR3 Antibody (C-term) in A2058 cell line lysates (35ug/lane). ACTR3 (arrow) was detected using the purified Pab.)
Related Product Information for anti-ACTR3 antibody
ACTR3 is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion.
Product Categories/Family for anti-ACTR3 antibody
References
Weisswange,I., et. al., Nature 458 (7234), 87-91 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47371
NCBI Official Full Name
actin-related protein 3 isoform 1
NCBI Official Synonym Full Names
ARP3 actin-related protein 3 homolog (yeast)
NCBI Official Symbol
ACTR3
NCBI Official Synonym Symbols
ARP3
NCBI Protein Information
actin-related protein 3
UniProt Protein Name
Actin-related protein 3
UniProt Gene Name
ACTR3
UniProt Synonym Gene Names
ARP3
UniProt Entry Name
ARP3_HUMAN
Similar Products
Product Notes
The ACTR3 actr3 (Catalog #AAA288516) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 380-407. The ACTR3 Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Chicken, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's ACTR3 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ACTR3 actr3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACTR3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.