Rabbit anti-Human Adenosine Deaminase Polyclonal Antibody | anti-ADA antibody

Anti-Adenosine Deaminase Antibody, Rabbit Polyclonal

Cat. Num. AAA254361

• Reactivity: Human
• Applications: Immunoprecipitation, Immunohistochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: Adenosine Deaminase, Antibody; Anti-Adenosine Deaminase Antibody, Rabbit Polyclonal; Adenosine Deaminase/ADA Antibody, Rabbit PAb, Antigen Affinity Purified; adenosine deaminase; Anti-ADA Antibody; Anti-ADA1 Antibody; anti-ADA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Adenosine Deaminase/ADA
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-ADA antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human Adenosine Deaminase/ADA
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Adenosine Deaminase/ADA, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(LIN7A was immunoprecipitated using:Lane A:0.5 mg mouse brain tissue lysate4 uL anti-LIN7A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-LIN7A rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size :28 kDa)

IHC (Immunohistochemisry)

(Immunochemical staining of human ADA in human duodenum with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohiostchemistry)

(Immunochemical staining of human ADA in human brain with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot)

(Anti-ADA rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:41 kDa)

Related Product Information for anti-ADA antibody

Adenosine Desaminase (ADA) deficiency, is a purine metabolic disorder that cause severe combined immunodeficiency (SCID) due to the accumulation of toxic metabolites that primarily affects development, differentiation and function of T and B lymphocytes. Adenosine deaminase is a polymorphic enzyme that has an important role in immune functions and in the regulation of intracellular and extracellular concentrations of adenosine and adenosine receptor activity. ADA activity might be considered as a useful tool among the other markers in these diseases. Genetic variability of ADA activity may have, therefore, an important role in resistance to malaria. Adenosine Deaminase (ADA) deficiency is an autosomal recessive variant of severe combined immunodeficiency (SCID) caused by systemic accumulation of ADA substrates.

NCBI and Uniprot Product Information

• NCBI GI #: 47078295
• NCBI GeneID: 100
• NCBI Accession #: NP_000013.2
• NCBI GenBank Nucleotide #: NM_000022.2
• UniProt Accession #: P00813; Q53F92; Q6LA59
• Molecular Weight: 40,764 Da
• NCBI Official Full Name: adenosine deaminase
• NCBI Official Synonym Full Names: adenosine deaminase
• NCBI Official Symbol: ADA
• NCBI Protein Information: adenosine deaminase; adenosine aminohydrolase
• UniProt Protein Name: Adenosine deaminase
• UniProt Gene Name: ADA
• UniProt Synonym Gene Names: ADA1
• UniProt Entry Name: ADA_HUMAN