FCM/FACS (Flow Cytometry)
(AGER Antibody (N-term) flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human, Mouse AGER Polyclonal Antibody | anti-AGER antibody
AGER Antibody (N-term)
Gene Names
AGER; RAGE
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
AGER, Antibody; AGER Antibody (N-term); Advanced glycosylation end product-specific receptor; Receptor for advanced glycosylation end products; AGER; RAGE; anti-AGER antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This AGER antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-52 amino acids from the N-terminal region of human AGER.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
0.5 mg/ml (varies by lot)
Sequence Positions
24-52
Applicable Applications for anti-AGER antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF- alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides.
Cellular Location
Isoform 1: Cell membrane; Single-pass type I membrane protein Isoform 10: Cell membrane; Single-pass type I membrane protein
Tissue Location
Endothelial cells
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(AGER Antibody (N-term) flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(AGER Antibody (N-term) flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(AGER Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AGER Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(AGER Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AGER Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of AGER Antibody (N-term) in mouse lung tissue lysates (35ug/lane). AGER (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of AGER Antibody (N-term) in mouse lung tissue lysates (35ug/lane). AGER (arrow) was detected using the purified Pab.)
Related Product Information for anti-AGER antibody
AGER encodes a member of the immunoglobulin superfamily of cell surface molecules. It is a receptor for various molecules, including the amyloidogenic form of serum amyloid A, amyloid-beta protein, members of the S100/calgranulin superfamily and advanced glycation end products. The gene lies within the major histocompatibility complex (MHC) class III region on chromosome 6.
Product Categories/Family for anti-AGER antibody
References
Rojas, A., et al. Carcinogenesis 31(3):334-341(2010)
Engelen, L., et al. J. Hypertens. 28(2):285-293(2010)
Poon, P.Y., et al. Clin. Nephrol. 73(1):44-50(2010)
Hancock, D.B., et al. Nat. Genet. 42(1):45-52(2010)
Engelen, L., et al. J. Hypertens. 28(2):285-293(2010)
Poon, P.Y., et al. Clin. Nephrol. 73(1):44-50(2010)
Hancock, D.B., et al. Nat. Genet. 42(1):45-52(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42803
NCBI Official Full Name
advanced glycosylation end product-specific receptor isoform 1
NCBI Official Synonym Full Names
advanced glycosylation end product-specific receptor
NCBI Official Symbol
AGER
NCBI Official Synonym Symbols
RAGE
NCBI Protein Information
advanced glycosylation end product-specific receptor
UniProt Protein Name
Advanced glycosylation end product-specific receptor
UniProt Gene Name
AGER
UniProt Synonym Gene Names
RAGE
UniProt Entry Name
RAGE_HUMAN
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Product Notes
The AGER ager (Catalog #AAA288108) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 24-52. The AGER Antibody (N-term) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AGER can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the AGER ager for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AGER, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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