<
WB (Western Blot)
(Figure 1. Western blot analysis of AKR7A2 using anti-AKR7A2 antibody.)
Rabbit AKR7A2 Polyclonal Antibody | anti-AKR7A2 antibody
Anti-AKR7A2 Antibody
Gene Names
AKR7A2; AFAR; AKR7; AFAR1; AFB1-AR1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
Purity
Immunogen affinity purified.
Synonyms
AKR7A2; Polyclonal Antibody; Anti-AKR7A2 Antibody; Aflatoxin B1 aldehyde reductase member 2; AFB1 aldehyde reductase 1; AFB1-AR 1; Aldoketoreductase 7; Succinic semialdehyde reductase; SSA reductase; AFAR; AFAR1; AKR7; anti-AKR7A2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
359
Applicable Applications for anti-AKR7A2 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), Direct ELISA (EIA)
Application Notes
WB: 0.25-0.5ug/ml (Human, Mouse, Rat)
IHC-P (Embedded Section): 0.5-1ug/ml (Human, Mouse, Rat)
FC/FACS: 1-3ug/1x106 cells (Human)
Direct ELISA: 0.1-0.5ug/ml (Human)
Tested Species: In-house tested species with positive results.
IHC-P (Embedded Section): 0.5-1ug/ml (Human, Mouse, Rat)
FC/FACS: 1-3ug/1x106 cells (Human)
Direct ELISA: 0.1-0.5ug/ml (Human)
Tested Species: In-house tested species with positive results.
Immunogen
E Coli-derived human AKR7A2 recombinant protein (Position: A294-R359).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Figure 1. Western blot analysis of AKR7A2 using anti-AKR7A2 antibody.)
WB (Western Blot)
(Figure 1. Western blot analysis of AKR7A2 using anti-AKR7A2 antibody.)
IHC (Immunohistochemistry)
IHC (Immunohistochemistry)
IHC (Immunohistochemistry)
IHC (Immunohistochemistry)
IHC (Immunohistochemistry)
IHC (Immunohistochemistry)
IHC (Immunohistochemistry)
IHC (Immunohistochemistry)
FCM (Flow Cytometry)
FCM (Flow Cytometry)
Related Product Information for anti-AKR7A2 antibody
Description: Rabbit IgG polyclonal antibody for AKR7A2 detection. Tested with WB, IHC-P, FCM, Direct ELISA in Human; Mouse; Rat.
Background: Aflatoxin B1 aldehyde reductase member 2 is an enzyme that in humans is encoded by the AKR7A2 gene. It is mapped to 1p36.13. The protein encoded by this gene belongs to the aldo/keto reductase (AKR) superfamily and AKR7 family, which are involved in the detoxification of aldehydes and ketones. The AKR7 family consists of 3 genes that are present in a cluster on the p arm of chromosome 1. This protein, thought to be localized in the golgi, catalyzes the NADPH-dependent reduction of succinic semialdehyde to the endogenous neuromodulator, gamma-hydroxybutyrate. It may also function as a detoxication enzyme in the reduction of aflatoxin B1 and 2-carboxybenzaldehyde. Alternative splicing results in multiple transcript variants.
Background: Aflatoxin B1 aldehyde reductase member 2 is an enzyme that in humans is encoded by the AKR7A2 gene. It is mapped to 1p36.13. The protein encoded by this gene belongs to the aldo/keto reductase (AKR) superfamily and AKR7 family, which are involved in the detoxification of aldehydes and ketones. The AKR7 family consists of 3 genes that are present in a cluster on the p arm of chromosome 1. This protein, thought to be localized in the golgi, catalyzes the NADPH-dependent reduction of succinic semialdehyde to the endogenous neuromodulator, gamma-hydroxybutyrate. It may also function as a detoxication enzyme in the reduction of aflatoxin B1 and 2-carboxybenzaldehyde. Alternative splicing results in multiple transcript variants.
References
1. Ireland, L. S., Harrison, D. J., Neal, G. E., Hayes, J. D. Molecular cloning, expression and catalytic activity of a human AKR7 member of the aldo-keto reductase superfamily: evidence that the major 2-carboxybenzaldehyde reductase from human liver is a homologue of rat aflatoxin B(1)-aldehyde reductase. Biochem. J. 332: 21-34, 1998.
2. Kelly, V. P., Sherratt, P. J., Crouch, D. H., Hayes, J. D. Novel homodimeric and heterodimeric rat gamma-hydroxybutyrate synthases that associate with the Golgi apparatus define a distinct subclass of reductase 7 family proteins. Biochem. J. 366: 847-861, 2002.
3. Praml, C., Savelyeva, L., Schwab, M. Aflatoxin B1 aldehyde reductase (AFAR) genes cluster at 1p35-1p36.1 in a region frequently altered in human tumour cells. Oncogene 22: 4765-4773, 2003.
2. Kelly, V. P., Sherratt, P. J., Crouch, D. H., Hayes, J. D. Novel homodimeric and heterodimeric rat gamma-hydroxybutyrate synthases that associate with the Golgi apparatus define a distinct subclass of reductase 7 family proteins. Biochem. J. 366: 847-861, 2002.
3. Praml, C., Savelyeva, L., Schwab, M. Aflatoxin B1 aldehyde reductase (AFAR) genes cluster at 1p35-1p36.1 in a region frequently altered in human tumour cells. Oncogene 22: 4765-4773, 2003.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Aflatoxin B1 aldehyde reductase member 2
NCBI Official Synonym Full Names
aldo-keto reductase family 7 member A2
NCBI Official Symbol
AKR7A2
NCBI Official Synonym Symbols
AFAR; AKR7; AFAR1; AFB1-AR1
NCBI Protein Information
aflatoxin B1 aldehyde reductase member 2
UniProt Protein Name
Aflatoxin B1 aldehyde reductase member 2
UniProt Gene Name
AKR7A2
UniProt Synonym Gene Names
AFAR; AFAR1; AKR7; AFB1-AR 1; SSA reductase
UniProt Entry Name
ARK72_HUMAN
Similar Products
Product Notes
The AKR7A2 akr7a2 (Catalog #AAA19208) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-AKR7A2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AKR7A2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), Direct ELISA (EIA). WB: 0.25-0.5ug/ml (Human, Mouse, Rat) IHC-P (Embedded Section): 0.5-1ug/ml (Human, Mouse, Rat) FC/FACS: 1-3ug/1x106 cells (Human) Direct ELISA: 0.1-0.5ug/ml (Human) Tested Species: In-house tested species with positive results. Researchers should empirically determine the suitability of the AKR7A2 akr7a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKR7A2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
