Rabbit anti-Human ALDH1A1 Polyclonal Antibody | anti-ALDH1A1 antibody

ALDH1A1 Antibody

Cat. Num. AAA288728

• Gene Names: ALDH1A1; ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Immunofluorescence
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: ALDH1A1, Antibody; ALDH1A1 Antibody; Retinal dehydrogenase 1; RALDH 1; RalDH1; ALDH-E1; ALHDII; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase; cytosolic; ALDH1A1; ALDC; ALDH1; PUMB1; anti-ALDH1A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Clone Number: RB23017
• Specificity: This ALDH1A1 antibody is generated from rabbits immunized with human ALDH1A1 recombinant protein.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 501

• Applicable Applications for anti-ALDH1A1 antibody: WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, IF (Immunofluorescence)
• Function: Can convert/oxidize retinaldehyde to retinoic acid. Binds free retinal and cellular retinol-binding protein-bound retinal (By similarity). May have a broader specificity and oxidize other aldehydes in vivo (PubMed: , PubMed:26373694, PubMed: ).
• Cellular Location: Cytoplasm, cytosol {ECO:0000250|UniProtKB:P48644}
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(ALDH1A1 Antibody flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemisry)

(ALDH1A1 Antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ALDH1A1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)

IF (Immunofluorescence)

(Fluorescent confocal image of HepG2 cells stained with ALDH1A1 antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA288728 ALDH1A1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ALDH1A1 immunoreactivity is localized to the cytoplasm of HepG2 cells.)

WB (Western Blot)

(Western blot analysis of lysates from HepG2 cell line and human lung tissue lysate (from left to right), using ALDH1A1 Antibody. AAA288728 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Related Product Information for anti-ALDH1A1 antibody

ALDH1A1 belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme.

Product Categories/Family for anti-ALDH1A1 antibody

Crown Antibodies; Cancer; Signal Transduction

References

References for protein:
1.Moore,S., J Stud Alcohol Drugs 68 (2), 192-196 (2007)
2.Collard,F., Biochimie 89 (3), 369-373 (2007)
References for HepG2 cell line:
1. Knowles BB, et al. (1980). Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499.[ PubMed: 6248960].
2. Darlington GJ, et al. (1987). Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354.[PubMed: 3034851].
3. Ihrke, G; Neufeld, EB; Meads, T; Shanks, MR; Cassio, D; Laurent, M; Schroer, TA; Pagano, RE et al. (1993). "WIF-B cells: an in vitro model for studies of hepatocyte polarity". Journal of Cell Biology 123 (6): 1761-1775. [PubMed:7506266].
4. Mersch-Sundermann, V.; Knasmüller, S.; Wu, X. J.; Darroudi, F.; Kassie, F. (2004). "Use of a human-derived liver cell line for the detection of cytoprotective, antigenotoxic and cogenotoxic agents". Toxicology 198 (1-3): 329-340. [PubMed:15138059].

NCBI and Uniprot Product Information

• NCBI GI #: 21361176
• NCBI GeneID: 216
• NCBI Accession #: NP_000680.2
• NCBI GenBank Nucleotide #: NM_000689.4
• UniProt Accession #: P00352; O00768; Q5SYR1
• NCBI Official Full Name: retinal dehydrogenase 1
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 1 family, member A1
• NCBI Official Symbol: ALDH1A1
• NCBI Official Synonym Symbols: ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e
• NCBI Protein Information: retinal dehydrogenase 1
• UniProt Protein Name: Retinal dehydrogenase 1
• UniProt Gene Name: ALDH1A1
• UniProt Synonym Gene Names: ALDC; ALDH1; PUMB1; RALDH 1; RalDH1
• UniProt Entry Name: AL1A1_HUMAN