IHC (Immunohiostchemistry)
(Anti-Alkaline Phosphatase antibody, AAA45154, IHC(P)IHC(P): Rat Small Intestine Tissue)
Rabbit Alkaline Phosphatase Polyclonal Antibody | anti-ALPL antibody
Anti-Alkaline Phosphatase antibody
Gene Names
ALPL; HOPS; TNAP; APTNAP; TNSALP; AP-TNAP
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Alkaline Phosphatase, Antibody; Anti-Alkaline Phosphatase antibody; Alkaline phosphatase, tissue-nonspecific isozyme(AP-TNAP/TNSALP); alkaline phosphatase, liver/bone/kidney; Akp2 antibody; Alkaline phosphatase intestinal antibody; Alkaline phosphatase intestinal precursor antibody; Alkaline phosphatase liver/bone/kidney antibody; Alkaline phosphatase placental antibody; Alkaline phosphatase placental like 2 antibody; Alkaline phosphatase placental like antibody; Alkaline phosphatase placental type antibody; Alkaline phosphomonoesterase antibody; ALP 1 antibody; ALP antibody; ALP I antibody; ALP L antibody; ALP P antibody; ALPG antibody; ALPI antibody; ALPL antibody; ALPP antibody; ALPPL antibody; ALPPL2 antibody; AP TNAP antibody; FLJ40094 antibody; GCAP antibody; Germ cell alkaline phosphatase antibody; Glycerophosphatase antibody; HOPS antibody; IAP antibody; Intestinal alkaline phosphatase antibody; Kasahara isozyme antibody; Nagao isozyme antibody; OTTHUMP00000164357 antibody; Placental alkaline phosphatase antibody; Placental like alkaline phosphatase antibody; PLAP 1 antibody; PLAP antibody; PLAP like antibody; Regan isozyme antibody; Testicular and thymus alkaline phosphatase antibody; Tissue non specific alkaline phosphatase antibody; Tissue nonspecific ALP antibody; TNAP antibody; TNSALP antibody; anti-ALPL antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
524
Applicable Applications for anti-ALPL antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Alkaline Phosphatase (21-35aa,EKEKDPKYWRDQAQE), different from the related rat and mouse sequences by two amino acids.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Anti-Alkaline Phosphatase antibody, AAA45154, IHC(P)IHC(P): Rat Small Intestine Tissue)
IHC (Immunohiostchemistry)
(Anti-Alkaline Phosphatase antibody, AAA45154, IHC(P)IHC(P): Rat Small Intestine Tissue)
WB (Western Blot)
(Anti-Alkaline Phosphatase antibody, AAA45154, Western blottingAll lanes: Anti ALPL (AAA45154) at 0.5ug/mlWB: HT1080 Whole Cell Lysate at 40ugPredicted bind size: 57KDObserved bind size: 57KD)
WB (Western Blot)
(Anti-Alkaline Phosphatase antibody, AAA45154, Western blottingAll lanes: Anti ALPL (AAA45154) at 0.5ug/mlWB: HT1080 Whole Cell Lysate at 40ugPredicted bind size: 57KDObserved bind size: 57KD)
Related Product Information for anti-ALPL antibody
Description: Rabbit IgG polyclonal antibody for Alkaline phosphatase, tissue-nonspecific isozyme(ALPL) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: Alkaline phosphatase(ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific(found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts,the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine(PEA) and pyridoxal-5-prime-phosphate(PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
Background: Alkaline phosphatase(ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific(found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts,the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine(PEA) and pyridoxal-5-prime-phosphate(PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
51,045 Da
NCBI Official Full Name
Alkaline phosphatase, tissue-nonspecific isozyme
NCBI Official Synonym Full Names
alkaline phosphatase, liver/bone/kidney
NCBI Official Symbol
ALPL
NCBI Official Synonym Symbols
HOPS; TNAP; APTNAP; TNSALP; AP-TNAP
NCBI Protein Information
alkaline phosphatase, tissue-nonspecific isozyme; glycerophosphatase; tissue-nonspecific ALP; alkaline phosphomonoesterase; liver/bone/kidney-type alkaline phosphatase; alkaline phosphatase liver/bone/kidney isozyme
UniProt Protein Name
Alkaline phosphatase, tissue-nonspecific isozyme
UniProt Gene Name
ALPL
UniProt Synonym Gene Names
AP-TNAP; TNSALP
UniProt Entry Name
PPBT_HUMAN
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Product Notes
The ALPL alpl (Catalog #AAA45154) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Alkaline Phosphatase antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Alkaline Phosphatase can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ALPL alpl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alkaline Phosphatase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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