IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALOX5AP antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit ALOX5AP Polyclonal Antibody | anti-ALOX5AP antibody
ALOX5AP Antibody (Center)
Gene Names
ALOX5AP; FLAP
Reactivity
Human (Predicted Reactivity: Bovine, Monkey, Mouse, Pig, Rabbit, Rat)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ALOX5AP, Antibody; ALOX5AP Antibody (Center); Arachidonate 5-lipoxygenase-activating protein; FLAP; MK-886-binding protein; ALOX5AP; anti-ALOX5AP antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Monkey, Mouse, Pig, Rabbit, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ALOX5AP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-117 amino acids from the Central region of human ALOX5AP.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
86-117
Sequence Length
161
Applicable Applications for anti-ALOX5AP antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Function
Required for leukotriene biosynthesis by ALOX5 (5- lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886,a compound that blocks the biosynthesis of leukotrienes.
Cellular Location
Nucleus membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane;Multi-pass membrane protein
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 Weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALOX5AP antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALOX5AP antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of anti-ALOX5AP Antibody (Center) in K562 cell line lysates (35ug/lane). ALOX5AP(arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-ALOX5AP Antibody (Center) in K562 cell line lysates (35ug/lane). ALOX5AP(arrow) was detected using the purified Pab.)
Related Product Information for anti-ALOX5AP antibody
ALOX5AP is a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation.
Product Categories/Family for anti-ALOX5AP antibody
References
Zintzaras,E., Am. J. Epidemiol. 169 (5), 523-532 (2009)
Crosslin,D.R., Hum. Genet. 125 (2), 217-229 (2009)
Steinhilber,D., Pharm Acta Helv 69 (1), 3-14 (1994)
Crosslin,D.R., Hum. Genet. 125 (2), 217-229 (2009)
Steinhilber,D., Pharm Acta Helv 69 (1), 3-14 (1994)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18157
NCBI Official Full Name
arachidonate 5-lipoxygenase-activating protein isoform 2
NCBI Official Synonym Full Names
arachidonate 5-lipoxygenase-activating protein
NCBI Official Symbol
ALOX5AP
NCBI Official Synonym Symbols
FLAP
NCBI Protein Information
arachidonate 5-lipoxygenase-activating protein
UniProt Protein Name
Arachidonate 5-lipoxygenase-activating protein
UniProt Gene Name
ALOX5AP
UniProt Synonym Gene Names
FLAP
UniProt Entry Name
AL5AP_HUMAN
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Product Notes
The ALOX5AP alox5ap (Catalog #AAA285185) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 86-117. The ALOX5AP Antibody (Center) reacts with Human (Predicted Reactivity: Bovine, Monkey, Mouse, Pig, Rabbit, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's ALOX5AP can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ALOX5AP alox5ap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALOX5AP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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