WB (Western Blot)
(Western Blot of alpha 1 Antitrypsin antibody.Lane 1: alpha 1 Antitrypsin under reducing conditions.Lane 2: none. Load: 1 ug per lane. alpha 1 Antitrypsin antibody at 1:3000 for overnight at 4 deg C. Dylight 488 conjugated Donkey anti Goat secondary antibody at 1:10,000 for 45 min at RT. Block: Blocking buffer overnight at 4 deg C. Size: 55kDa for a-1 Anti-Trypsin.)
Goat alpha 1 Trypsin Polyclonal Antibody | anti-SERPINA1 antibody
alpha 1 Trypsin antibody
Gene Names
SERPINA1; PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
Applications
Western Blot, ELISA
Purity
Alpha 1 Trypsin antibody was purified by ion exchange chromatography followed by dialysis.
Synonyms
alpha 1 Trypsin, Antibody; alpha 1 Trypsin antibody; Polyclonal alpha 1 Trypsin; Anti-alpha 1 Trypsin; alpha Trypsin-1; A1AT; alpha Trypsin 1; alpha 1 antiproteinase; alpha 1 Trypsin; AAT; alpha 1 antitrypsin; alpha 1 protease inhibitor antibod; anti-SERPINA1 antibody
Host
Goat
Clonality
Polyclonal
Specificity
human alpha-1-trypsin
Purity/Purification
Alpha 1 Trypsin antibody was purified by ion exchange chromatography followed by dialysis.
Form/Format
Lyophilized from 20mM Potassium Phosphate, pH7.2, with 150mM Sodium Chloride, and 0.01% NaN3. Reconstitute with deionized water (or equivalent).
Concentration
100ug, Lyophilized (varies by lot)
Sequence Length
418
Applicable Applications for anti-SERPINA1 antibody
WB (Western Blot), ELISA
Biological Significance
Alpha 1-Antitrypsin or alpha1-antitrypsin (A1AT) is a protease inhibitor belonging to the serpin superfamily. It is generally known as serum trypsin inhibitor. Alpha 1-antitrypsin is also referred to as alpha-1 proteinase inhibitor (A1PI) because it inhibits a wide variety of proteases.
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Immunogen
a1-Ati-Trypsin (Human Plasma)
Cross Reactivity
Human
Preparation and Storage
Ships ambient or refrigerated/frozen. Upon receipt store at 4°C prior to restoration. After reconstituted , store at 4°C short term, or aliquot and freeze at -20°C or below for long term storage. Avoid repeat freeze-thaws.
WB (Western Blot)
(Western Blot of alpha 1 Antitrypsin antibody.Lane 1: alpha 1 Antitrypsin under reducing conditions.Lane 2: none. Load: 1 ug per lane. alpha 1 Antitrypsin antibody at 1:3000 for overnight at 4 deg C. Dylight 488 conjugated Donkey anti Goat secondary antibody at 1:10,000 for 45 min at RT. Block: Blocking buffer overnight at 4 deg C. Size: 55kDa for a-1 Anti-Trypsin.)
WB (Western Blot)
(Western Blot of alpha 1 Antitrypsin antibody.Lane 1: alpha 1 Antitrypsin under reducing conditions.Lane 2: none. Load: 1 ug per lane. alpha 1 Antitrypsin antibody at 1:3000 for overnight at 4 deg C. Dylight 488 conjugated Donkey anti Goat secondary antibody at 1:10,000 for 45 min at RT. Block: Blocking buffer overnight at 4 deg C. Size: 55kDa for a-1 Anti-Trypsin.)
WB (Western Blot)
(Western Blot of Trypsin antibody-Multiplex Fluorescent Western blot.Lane 1: molecular weight.Lane 2: alpha 1 Antitrypsin (red), Transferrin, and Mouse anti GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 ug per lane. Each primary antibody at 1:1000 for overnight at 4 deg C. DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. Block: Blocking buffer at 30 min RT)
WB (Western Blot)
(Western Blot of Trypsin antibody-Multiplex Fluorescent Western blot.Lane 1: molecular weight.Lane 2: alpha 1 Antitrypsin (red), Transferrin, and Mouse anti GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 ug per lane. Each primary antibody at 1:1000 for overnight at 4 deg C. DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. Block: Blocking buffer at 30 min RT)
WB (Western Blot)
(Western Blot of Alpha 1 anti trypsin antibody.Lane 1: molecular weight.Lane 2: Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Load: 35 ug per lane. Each primary antibody was diluted to 1:1000 for overnight at 4°C. Rabbit IgG (H&L) antibody DyLight649 Conjugated (green), Goat IgG (H&L) antibody DyLight549 Conjugated (red), Mouse IgG (H&L) antibody DyLight488 Conjugated (blue)secondary antibody at 1:10,000 for ~ 1hr at 4 degrees. Block: IRdye blocking buffer overnight at 4 deg C)
WB (Western Blot)
(Western Blot of Alpha 1 anti trypsin antibody.Lane 1: molecular weight.Lane 2: Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Load: 35 ug per lane. Each primary antibody was diluted to 1:1000 for overnight at 4°C. Rabbit IgG (H&L) antibody DyLight649 Conjugated (green), Goat IgG (H&L) antibody DyLight549 Conjugated (red), Mouse IgG (H&L) antibody DyLight488 Conjugated (blue)secondary antibody at 1:10,000 for ~ 1hr at 4 degrees. Block: IRdye blocking buffer overnight at 4 deg C)
Product Categories/Family for anti-SERPINA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
alpha-1-antitrypsin
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
NCBI Official Symbol
SERPINA1
NCBI Official Synonym Symbols
PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
NCBI Protein Information
alpha-1-antitrypsin
UniProt Protein Name
Alpha-1-antitrypsin
UniProt Gene Name
SERPINA1
UniProt Synonym Gene Names
AAT; PI; SPAAT
UniProt Entry Name
A1AT_HUMAN
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Product Notes
The SERPINA1 serpina1 (Catalog #AAA74945) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's alpha 1 Trypsin can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the SERPINA1 serpina1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha 1 Trypsin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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