Rabbit anti-Mouse alpha-2 macroglobulin Polyclonal Antibody | anti-A2M antibody
Rabbit anti Mouse alpha-2 macroglobulin
Gene Names
A2M; A2MD; CPAMD5; FWP007; S863-7
Reactivity
Mouse
Applications
Immunoprecipitation
Synonyms
alpha-2 macroglobulin, Antibody; Rabbit anti Mouse alpha-2 macroglobulin; anti-A2M antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
Precipitating polyclonal Rabbit antiSerum to Mouse alpha-2 macroglobulin
Applicable Applications for anti-A2M antibody
IP (Immunoprecipitation)
Source
Alpha-2 macroglobulin ids the major protein in the alpha-2 region of mouse serum after separation by electrophoresis. It is a large polymer and a strong antigen. It forms about one-third in the total of the alpha-2 globulins. The protein is isolated by ammonium sulphate precipitation, ion exchange chromatography, gel filtration and preparative starch block electrophoresis. In the purification of alpha-2 macroglobulin the activation of kallikrein is avoided, since it forms an irreversible bond with aplpha-2 macroglobulin, thus contaminating the final immunogen. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Product
Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Reconstitute the lyophilized antiserum by adding 1mL sterile water.
Reactivity Note
Inter-species cross reactivity is a normal feature of antibodies to Serum proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has been tested in double radial immunodiffusion (Ouchterlony) with the following results: Bovine +/- ferret +/- Horse +/- Rat ++ Cat +/- Goat - Human -Sheep - Chicken - Guinea Pig +/- Monkey - Swine +/- Dog - Hamster + pigeon - Turkey -
Preparation and Storage
Lyophilized store at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
Related Product Information for anti-A2M antibody
The reactivity of the antiserum is restricted to alpha-2 macroglobulin as tested in immunoelectrophoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified alpha-2 macroglobulin. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence alpha-2 macroglobulin in mouse serum or other body fluids and to determine its concentration. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays, but this does not exclude such use if proper controls are included.
Product Categories/Family for anti-A2M antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
alpha-2-macroglobulin
NCBI Official Synonym Full Names
alpha-2-macroglobulin
NCBI Official Symbol
A2M
NCBI Official Synonym Symbols
A2MD; CPAMD5; FWP007; S863-7
NCBI Protein Information
alpha-2-macroglobulin; alpha-2-M; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
UniProt Protein Name
Alpha-2-macroglobulin
UniProt Gene Name
A2M
UniProt Synonym Gene Names
CPAMD5; Alpha-2-M
UniProt Entry Name
A2MG_HUMAN
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Product Notes
The A2M a2m (Catalog #AAA77704) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Mouse alpha-2 macroglobulin reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's alpha-2 macroglobulin can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation). Researchers should empirically determine the suitability of the A2M a2m for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha-2 macroglobulin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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