IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using alpha Actinin 4 antibody at 1: 200 dilution.)
Rabbit anti-Human alpha Actinin 4 Polyclonal Antibody | anti-ACTN4 antibody
alpha Actinin 4 antibody
Gene Names
ACTN4; FSGS; FSGS1; ACTININ-4
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by antigen-affinity chromatography.
Synonyms
alpha Actinin 4, Antibody; alpha Actinin 4 antibody; anti-ACTN4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Purified by antigen-affinity chromatography.
Form/Format
Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
Sequence Length
911
Applicable Applications for anti-ACTN4 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Type
Recombinant protein
Immunogen Description
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 206 and 512 of alpha Actinin 4
Target Name
alpha Actinin 4
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using alpha Actinin 4 antibody at 1: 200 dilution.)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using alpha Actinin 4 antibody at 1: 200 dilution.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded A549 xenograft, using alpha Actinin 4 antibody at 1: 500 dilution.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded A549 xenograft, using alpha Actinin 4 antibody at 1: 500 dilution.)
Application Data
(Sample (30 ug of whole cell lysate) A: A431 B: Hep G2 7.5% SDS PAGE Primary antibody diluted at 1: 1000)
Application Data
(Sample (30 ug of whole cell lysate) A: A431 B: Hep G2 7.5% SDS PAGE Primary antibody diluted at 1: 1000)
Related Product Information for anti-ACTN4 antibody
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq]
Product Categories/Family for anti-ACTN4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
59,579 Da
NCBI Official Full Name
Actinin, alpha 4
NCBI Official Synonym Full Names
actinin, alpha 4
NCBI Official Symbol
ACTN4
NCBI Official Synonym Symbols
FSGS; FSGS1; ACTININ-4
NCBI Protein Information
alpha-actinin-4
UniProt Protein Name
Alpha-actinin-4
UniProt Gene Name
ACTN4
UniProt Entry Name
ACTN4_HUMAN
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Product Notes
The ACTN4 actn4 (Catalog #AAA302010) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The alpha Actinin 4 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's alpha Actinin 4 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ACTN4 actn4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha Actinin 4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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