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IHC (Immunohistochemistry)
(Immunohistochemical staining of paraffin-embedded A549 xenograft using alpha Actinin 4 antibody at a dilution of 1:500)
Rabbit alpha Actinin 4 Polyclonal Antibody | anti-ACTN4 antibody
alpha Actinin 4 antibody
Gene Names
ACTN4; FSGS; FSGS1; ACTININ-4
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
alpha Actinin 4 antibody was purified by antigen-affinity chromatography
Synonyms
alpha Actinin 4, Antibody; alpha Actinin 4 antibody; Polyclonal alpha Actinin 4; Anti-alpha Actinin 4; FSGS1; alpha Actinin 4; alpha Actinin -4; ACTININ-4; focal segmental glomerulosclerosis 1; DKFZp686K23158; FSGS; ACTN4; F-actin cross-linking protein; anti-ACTN4 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
alpha Actinin 4 antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1.03 mg/ml (varies by lot)
Sequence Length
911
Applicable Applications for anti-ACTN4 antibody
WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence)
Biological Significance
alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.
Cross-Reactivity
Human, Mouse
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
IHC (Immunohistochemistry)
(Immunohistochemical staining of paraffin-embedded A549 xenograft using alpha Actinin 4 antibody at a dilution of 1:500)
IHC (Immunohistochemistry)
(Immunohistochemical staining of paraffin-embedded A549 xenograft using alpha Actinin 4 antibody at a dilution of 1:500)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using alpha Actinin 4 antibody at 1:200 dilution.)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using alpha Actinin 4 antibody at 1:200 dilution.)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: A431; B: Hep G2) using a 7.5 % SDS PAGE gel and alpha Actinin 4 antibody at a dilution of 1:1000)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: A431; B: Hep G2) using a 7.5 % SDS PAGE gel and alpha Actinin 4 antibody at a dilution of 1:1000)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3) using a 7.5 % SDS PAGE gel and alpha Actinin 4 antibody at a dilution of 1:1000)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3) using a 7.5 % SDS PAGE gel and alpha Actinin 4 antibody at a dilution of 1:1000)
Related Product Information for anti-ACTN4 antibody
Affinity purified Rabbit polyclonal alpha Actinin 4 antibody
Product Categories/Family for anti-ACTN4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
105 kDa
NCBI Official Full Name
alpha-actinin-4
NCBI Official Synonym Full Names
actinin, alpha 4
NCBI Official Symbol
ACTN4
NCBI Official Synonym Symbols
FSGS; FSGS1; ACTININ-4
NCBI Protein Information
alpha-actinin-4
UniProt Protein Name
Alpha-actinin-4
UniProt Gene Name
ACTN4
UniProt Entry Name
ACTN4_HUMAN
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Product Notes
The ACTN4 actn4 (Catalog #AAA107633) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's alpha Actinin 4 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the ACTN4 actn4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha Actinin 4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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