WB (Western Blot)
(Detection of human Alpha-adaptin by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Alpha-adaptin antibody AAA212885 (lot AAA212885-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human Alpha-adaptin Polyclonal Antibody | anti-AP2A1 antibody
Rabbit anti-Alpha-adaptin Antibody, Affinity Purified
Gene Names
AP2A1; ADTAA; CLAPA1; AP2-ALPHA
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Alpha-adaptin, Antibody; Rabbit anti-Alpha-adaptin Antibody, Affinity Purified; alpha-adaptin A; plasma membrane adaptor HA2/AP2 adaptin alpha A subunit; clathrin-associated/assembly/adaptor protein, large, alpha 1; clathrin assembly protein complex 2 alpha-A large chain; CLAPA1; AP-2 complex subunit alpha-1; alpha1-adaptin; Adaptor-related protein complex 2 subunit alpha-1; adaptor related protein complex 2 alpha 1 subunit; adaptor protein complex AP-2 subunit alpha-1; adaptin, alpha A; adapter-related protein complex 2 subunit alpha-1; adapter-related protein complex 2 alpha-1 subunit; 100 kDa coated vesicle protein A; ADTAA; AP2-ALPHA; adaptor related protein complex 2 subunit alpha 1; anti-AP2A1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-AP2A1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 625 and 675
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Alpha-adaptin by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Alpha-adaptin antibody AAA212885 (lot AAA212885-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human Alpha-adaptin by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Alpha-adaptin antibody AAA212885 (lot AAA212885-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Alpha-adaptin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Alpha-adaptin antibody AAA212885 (lot AAA212885-1) used for IP at 6 ug per reaction. Alpha-adaptin was also immunoprecipitated by rabbit anti-Alpha-adaptin antibody BL18371. For blotting immunoprecipitated Alpha-adaptin, AAA212885 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Alpha-adaptin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Alpha-adaptin antibody AAA212885 (lot AAA212885-1) used for IP at 6 ug per reaction. Alpha-adaptin was also immunoprecipitated by rabbit anti-Alpha-adaptin antibody BL18371. For blotting immunoprecipitated Alpha-adaptin, AAA212885 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-AP2A1 antibody
Alpha-adaptin A is a subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles [taken from NCBI Entrez Gene (Gene ID: 160)].
Product Categories/Family for anti-AP2A1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
107,546 Da
NCBI Official Full Name
AP-2 complex subunit alpha-1 isoform 2
NCBI Official Synonym Full Names
adaptor-related protein complex 2, alpha 1 subunit
NCBI Official Symbol
AP2A1
NCBI Official Synonym Symbols
ADTAA; CLAPA1; AP2-ALPHA
NCBI Protein Information
AP-2 complex subunit alpha-1; alpha1-adaptin; alpha-adaptin A; adaptin, alpha A; 100 kDa coated vesicle protein A; adaptor protein complex AP-2 subunit alpha-1; adapter-related protein complex 2 alpha-1 subunit; clathrin assembly protein complex 2 alpha-A
UniProt Protein Name
AP-2 complex subunit alpha-1
UniProt Gene Name
AP2A1
UniProt Synonym Gene Names
ADTAA; CLAPA1
UniProt Entry Name
AP2A1_HUMAN
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Product Notes
The AP2A1 ap2a1 (Catalog #AAA212885) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Alpha-adaptin Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Alpha-adaptin can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the AP2A1 ap2a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha-adaptin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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