Goat AP2A1 / AP2-Alpha Polyclonal Antibody | anti-AP2A1 antibody

Goat Polyclonal to Human AP2A1 / AP2-Alpha

Cat. Num. AAA52380

• Gene Names: AP2A1; ADTAA; CLAPA1; AP2-ALPHA
• Reactivity: Gibbon, Chimpanzee, Dog, Gorilla, Horse, Human, Monkey, Mouse, Pig, Rabbit
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: AP2A1 / AP2-Alpha, Antibody; Goat Polyclonal to Human AP2A1 / AP2-Alpha; Anti-AP2A1 / AP2-Alpha Antibody (aa706-727) IHC-plus; AP2A1; Adaptin; alpha A; ADTAA; Alpha-adaptin A; Alpha1-adaptin; AP-2 complex subunit alpha-1; AP2 Alpha; CLAPA1; AP2-alpha; Human AP2A1; AP2-Alpha; anti-AP2A1 antibody

• Host: Goat
• Reactivity: Gibbon, Chimpanzee, Dog, Gorilla, Horse, Human, Monkey, Mouse, Pig, Rabbit
• Clonality: Polyclonal
• Specificity: Human AP2A1 / AP2-Alpha. This antibody is expected to recognize reported isoform 1 (NP_055018.2) only.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 977

• Applicable Applications for anti-AP2A1 antibody: ELISA, WB (Western Blot), IHC (Immunohistochemistry)
• Target Species: Human
• Immunogen Description: Synthetic peptide C-ELEPPAPESPMALLADPAPAAD from an internal region of human AP2A1 / AP2-Alpha (NP_055018.2).
• Immunogen Type: Synthetic peptide
• Immunogen: AP2A1 / AP2-Alpha antibody was raised against synthetic peptide C-ELEPPAPESPMALLADPAPAAD from an internal region of human AP2A1 / AP2-Alpha (NP_055018.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Ferret, Dog, Cat, Panda, Horse, Rabbit, Pig (100%); Rat (95%); Opossum, Guinea pig (91%).
• Antigen Modification: aa706-727
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

WB (Western Blot)

(AP2A1 antibody (0.01 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

WB (Western Blot)

(AP2A1 antibody (0.1 ug/ml) staining of Mouse Brain lysate (~5 ug protein in SDSPAGE buffer). The left panel shows the same blot stained with Ponceau red for total protein stain as the loading control before labeling. Primary incubation was 1 hour. Detected by chemiluminescence. Data obtained from Prof. M Robinson, CIMR, Cambridge, UK)

ICC (Immunocytochemistry)

(AP2A1 antibody (0.1 ug/ml) staining of methanol-fixed HeLa cells with stably expressing Mouse Ap2a1. Primary incubation was 1 hour. Detected by Alexa Fluor 594. Data obtained from Prof. M Robinson, CIMR, Cambridge, UK)

IHC (Immunohistochemistry)

(Anti-AP2A1 / AP2-Alpha antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Related Product Information for anti-AP2A1 antibody

This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms.

NCBI and Uniprot Product Information

• NCBI GI #: 19913414
• NCBI GeneID: 160
• NCBI Accession #: NP_055018.2
• NCBI GenBank Nucleotide #: NM_014203.2
• UniProt Accession #: O95782; Q96CI7; Q96PP6; Q96PP7; Q9H070
• Molecular Weight: 105,361 Da
• NCBI Official Full Name: AP-2 complex subunit alpha-1 isoform 1
• NCBI Official Synonym Full Names: adaptor-related protein complex 2, alpha 1 subunit
• NCBI Official Symbol: AP2A1
• NCBI Official Synonym Symbols: ADTAA; CLAPA1; AP2-ALPHA
• NCBI Protein Information: AP-2 complex subunit alpha-1; alpha1-adaptin; alpha-adaptin A; adaptin, alpha A; 100 kDa coated vesicle protein A; adaptor protein complex AP-2 subunit alpha-1; adapter-related protein complex 2 alpha-1 subunit; adapter-related protein complex 2 subunit alpha-1; adaptor-related protein complex 2 subunit alpha-1; clathrin assembly protein complex 2 alpha-A large chain; plasma membrane adaptor HA2/AP2 adaptin alpha A subunit; clathrin-associated/assembly/adaptor protein, large, alpha 1
• UniProt Protein Name: AP-2 complex subunit alpha-1
• UniProt Gene Name: AP2A1
• UniProt Synonym Gene Names: ADTAA; CLAPA1
• UniProt Entry Name: AP2A1_HUMAN