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product-image-AAA285124_FCM11.jpg FCM/FACS (Flow Cytometry) (APITD1 Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Rabbit APITD1 Polyclonal Antibody | anti-APITD1 antibody

APITD1 Antibody (Center)

Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
APITD1, Antibody; APITD1 Antibody (Center); Centromere protein S; CENP-S; Apoptosis-inducing TAF9-like domain-containing protein 1; FANCM-interacting histone fold protein 1; Fanconi anemia-associated polypeptide of 16 kDa; APITD1; CENPS; FAAP16; MHF1; anti-APITD1 antibody
Ordering
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This APITD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-74 amino acids from the Central region of human APITD1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
48-74
Sequence Length
77
Applicable Applications for anti-APITD1 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(APITD1 Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

product-image-AAA285124_FCM11.jpg FCM/FACS (Flow Cytometry) (APITD1 Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(APITD1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the APITD1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

product-image-AAA285124_IHC13.jpg IHC (Immunohiostchemistry) (APITD1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the APITD1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of APITD1 Antibody (Center) in mouse NIH-3T3 cell line lysates (35ug/lane). APITD1 (arrow) was detected using the purified Pab.)

product-image-AAA285124_WB15.jpg WB (Western Blot) (Western blot analysis of APITD1 Antibody (Center) in mouse NIH-3T3 cell line lysates (35ug/lane). APITD1 (arrow) was detected using the purified Pab.)
Related Product Information for anti-APITD1 antibody
APITD1 was identified in the neuroblastoma tumour suppressor candidate region on chromosome 1p36. It contains a TFIID-31 domain, similar to that found in TATA box-binding protein-associated factor, TAF(II)31, which is required for p53-mediated transcription activation. This gene was expressed at very low levels in neuroblastoma tumours, and was shown to reduce cell growth in neuroblastoma cells, suggesting that it may have a role in a cell death pathway.
Product Categories/Family for anti-APITD1 antibody
References
Amano, M., et al. J. Cell Biol. 186(2):173-182(2009)
van Gils, W., et al. Invest. Ophthalmol. Vis. Sci. 48(11):4919-4923(2007)
Okada, M., et al. Nat. Cell Biol. 8(5):446-457(2006)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15893
NCBI Official Full Name
APITD1-CORT protein isoform 4
NCBI Official Synonym Full Names
APITD1-CORT readthrough
NCBI Official Symbol
APITD1-CORT
NCBI Protein Information
APITD1-CORT protein
UniProt Protein Name
Centromere protein S
UniProt Gene Name
APITD1
UniProt Synonym Gene Names
CENPS; FAAP16; MHF1; CENP-S
UniProt Entry Name
CENPS_HUMAN

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Product Notes

The APITD1 apitd1 (Catalog #AAA285124) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 48-74. The APITD1 Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's APITD1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the APITD1 apitd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APITD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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