IP (Immunoprecipitation)
(Detection of human ARNT by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ARNT antibody AAA212002 used for IP at 3 ug/mg lysate. ARNT was also immunoprecipitated by rabbit anti-ARNT antibody which recognizes a downstream epitope. For blotting immunoprecipitated ARNT, AAA212002 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human ARNT Polyclonal Antibody | anti-ARNT antibody
Rabbit anti-ARNT Antibody, Affinity Purified
Gene Names
ARNT; HIF1B; TANGO; bHLHe2; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
ARNT, Antibody; Rabbit anti-ARNT Antibody, Affinity Purified; HIF-1beta; class E basic helix-loop-helix protein 2; TANGO; Hypoxia-inducible factor 1-beta; hypoxia-inducible factor 1, beta subunit; HIF1-beta; HIF-1-beta; HIF1BETA; dioxin receptor, nuclear translocator; bHLHe2; aryl hydrocarbon receptor nuclear translocator; ARNT protein; HIF1B; Aryl hydrocarbon receptor nuclear translocator; anti-ARNT antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ARNT antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Predicted Reactivity
Bovine
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human ARNT by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ARNT antibody AAA212002 used for IP at 3 ug/mg lysate. ARNT was also immunoprecipitated by rabbit anti-ARNT antibody which recognizes a downstream epitope. For blotting immunoprecipitated ARNT, AAA212002 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human ARNT by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ARNT antibody AAA212002 used for IP at 3 ug/mg lysate. ARNT was also immunoprecipitated by rabbit anti-ARNT antibody which recognizes a downstream epitope. For blotting immunoprecipitated ARNT, AAA212002 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human ARNT by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ARNT (Cat. No. AAA212002 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human ARNT by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ARNT (Cat. No. AAA212002 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-ARNT antibody
Aryl hydrocarbon receptor nuclear translocator (ARNT) is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. ARNT forms a complex with the ligand-bound Ah receptor, and is required for receptor function. ARNT has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia [taken from NCBI Entrez Gene (GeneID: 405)].
Product Categories/Family for anti-ARNT antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
86,636 Da
NCBI Official Full Name
aryl hydrocarbon receptor nuclear translocator isoform 1
NCBI Official Synonym Full Names
aryl hydrocarbon receptor nuclear translocator
NCBI Official Symbol
ARNT
NCBI Official Synonym Symbols
HIF1B; TANGO; bHLHe2; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta
NCBI Protein Information
aryl hydrocarbon receptor nuclear translocator; dioxin receptor, nuclear translocator; class E basic helix-loop-helix protein 2; hypoxia-inducible factor 1, beta subunit
UniProt Protein Name
Aryl hydrocarbon receptor nuclear translocator
UniProt Gene Name
ARNT
UniProt Synonym Gene Names
BHLHE2; ARNT protein; bHLHe2; HIF-1-beta; HIF1-beta
UniProt Entry Name
ARNT_HUMAN
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Product Notes
The ARNT arnt (Catalog #AAA212002) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ARNT Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARNT can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the ARNT arnt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARNT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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