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IHC (Immunohistochemistry)
(Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (ASB10) Polyclonal Antibody, Unconjugated (bs-4653R) at 1:200 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
Rabbit ASB10 Polyclonal Antibody | anti-ASB10 antibody
ASB10 Polyclonal Antibody
Gene Names
ASB10; ASB10
Reactivity
Rat, Pig, Dog, Cow
Applications
Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ASB10, Antibody; ASB10 Polyclonal Antibody; Ankyrin repeat and SOCS box protein 10; ASB-10; ASB10; anti-ASB10 antibody
Host
Rabbit
Reactivity
Rat, Pig, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-ASB10 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemistry)
(Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (ASB10) Polyclonal Antibody, Unconjugated (bs-4653R) at 1:200 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
IHC (Immunohistochemistry)
(Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (ASB10) Polyclonal Antibody, Unconjugated (bs-4653R) at 1:200 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
WB (Western Blot)
(Dilution: Sample:Heart (Mouse) Lysate at 40 ugMuscle (Mouse) Lysate at 40 ugPrimary: Anti- ASB10 (bs-4653R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 51 kDObserved band size: 51 kD)
WB (Western Blot)
(Dilution: Sample:Heart (Mouse) Lysate at 40 ugMuscle (Mouse) Lysate at 40 ugPrimary: Anti- ASB10 (bs-4653R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 51 kDObserved band size: 51 kD)
WB (Western Blot)
(Sample:Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti- ASB10 (bs-4653R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 51 kDObserved band size: 51 kD)
WB (Western Blot)
(Sample:Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti- ASB10 (bs-4653R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 51 kDObserved band size: 51 kD)
WB (Western Blot)
(Sample:A431(Human) Cell Lysate at 30 ugPrimary: Anti- ASB10 (bs-4653R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 51 kDObserved band size: 53 kD)
WB (Western Blot)
(Sample:A431(Human) Cell Lysate at 30 ugPrimary: Anti- ASB10 (bs-4653R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 51 kDObserved band size: 53 kD)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
ankyrin repeat and SOCS box protein 10 isoform 2 [Homo sapiens]
NCBI Official Synonym Full Names
ankyrin repeat and SOCS box-containing 10
NCBI Official Symbol
ASB10
NCBI Official Synonym Symbols
ASB10
NCBI Protein Information
ankyrin repeat and SOCS box protein 10; OTTHUMP00000211301
UniProt Protein Name
Ankyrin repeat and SOCS box protein 10
UniProt Gene Name
ASB10
UniProt Entry Name
ASB10_HUMAN
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Product Notes
The ASB10 asb10 (Catalog #AAA291989) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ASB10 Polyclonal Antibody reacts with Rat, Pig, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's ASB10 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ASB10 asb10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ASB10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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