FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U87 cells using anti-KCNJ16 antibody (AAA125815).Overlay histogram showing U87 cells stained with AAA125815 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KCNJ16 Antibody (AAA125815, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit KCNJ16 Polyclonal Antibody | anti-KCNJ16 antibody
Anti-KCNJ16 Antibody
Gene Names
KCNJ16; BIR9; KIR5.1
Reactivity
Human, Monkey
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
KCNJ16, Antibody; Anti-KCNJ16 Antibody; KCNJ16; Inward rectifier potassium channel 16; Inward rectifier K(+ channel Kir5. 1; Potassium channel, inwardly rectifying subfamily J member 16; potassium inwardly rectifying channel subfamily J member 16; anti-KCNJ16 antibody
Host
Rabbit
Reactivity
Human, Monkey
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rabbit IgG polyclonal antibody for KCNJ16 detection.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Applicable Applications for anti-KCNJ16 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
E Coli-derived human KCNJ16 recombinant protein (Position: M1-M418).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U87 cells using anti-KCNJ16 antibody (AAA125815).Overlay histogram showing U87 cells stained with AAA125815 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KCNJ16 Antibody (AAA125815, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U87 cells using anti-KCNJ16 antibody (AAA125815).Overlay histogram showing U87 cells stained with AAA125815 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KCNJ16 Antibody (AAA125815, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of KCNJ16 using anti-KCNJ16 antibody (AAA125815).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human CACO-2 whole cell lysatesLane 2: human HEK293 whole cell lysatesLane 3: human HL-60 whole cell lysatesLane 4: monkey kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-KCNJ16 antigen affinity purified polyclonal antibody (Catalog # AAA125815) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KCNJ16 at approximately 48KD. The expected band size for KCNJ16 is at 48KD.)
WB (Western Blot)
(Figure 1. Western blot analysis of KCNJ16 using anti-KCNJ16 antibody (AAA125815).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human CACO-2 whole cell lysatesLane 2: human HEK293 whole cell lysatesLane 3: human HL-60 whole cell lysatesLane 4: monkey kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-KCNJ16 antigen affinity purified polyclonal antibody (Catalog # AAA125815) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KCNJ16 at approximately 48KD. The expected band size for KCNJ16 is at 48KD.)
Related Product Information for anti-KCNJ16 antibody
Potassium inwardly-rectifying channel, subfamily J, member 16 (KCNJ16) is a human gene encoding the Kir5. 1 protein. Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which tends to allow potassium to flow into rather than out of a cell, can form heterodimers with two other inward-rectifier type potassium channels. It may function in fluid and pH balance regulation. Alternatively spliced transcript variants have been found for this gene.
References
1. D'Adamo, M. C., Shang, L., Imbrici, P., Brown, S. D. M., Pessia, M., Tucker, S. J. Genetic inactivation of Kcnj16 identifies Kir5. 1 as an important determinant of neuronal PCO2/pH sensitivity. J. Biol. Chem. 286: 192-198, 2011.
2. Derst, C., Karschin, C., Wischmeyer, E., Hirsch, J. R., Preisig-Muller, R., Rajan, S., Engel, H., Grzeschik, K. -H., Daut, J., Karschin, A. Genetic and functional linkage of Kir5. 1 and Kir2. 1 channel subunits. FEBS Lett. 491: 305-311, 2001.
3. Liu, Y., McKenna, E., Figueroa, D. J., Blevins, R., Austin, C. P., Bennett, P. B., Swanson, R. The human inward rectifier K+ channel subunit Kir5. 1 (KCNJ16) maps to chromosome 17q25 and is expressed in kidney and pancreas. Cytogenet. Cell Genet. 90: 60-63, 2000.
2. Derst, C., Karschin, C., Wischmeyer, E., Hirsch, J. R., Preisig-Muller, R., Rajan, S., Engel, H., Grzeschik, K. -H., Daut, J., Karschin, A. Genetic and functional linkage of Kir5. 1 and Kir2. 1 channel subunits. FEBS Lett. 491: 305-311, 2001.
3. Liu, Y., McKenna, E., Figueroa, D. J., Blevins, R., Austin, C. P., Bennett, P. B., Swanson, R. The human inward rectifier K+ channel subunit Kir5. 1 (KCNJ16) maps to chromosome 17q25 and is expressed in kidney and pancreas. Cytogenet. Cell Genet. 90: 60-63, 2000.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47,949 Da
NCBI Official Full Name
inward rectifier potassium channel 16
NCBI Official Synonym Full Names
potassium inwardly-rectifying channel, subfamily J, member 16
NCBI Official Symbol
KCNJ16
NCBI Official Synonym Symbols
BIR9; KIR5.1
NCBI Protein Information
inward rectifier potassium channel 16; inward rectifier K+ channel KIR5.1; inward rectifier K(+) channel Kir5.1; potassium channel, inwardly rectifying subfamily J member 16
UniProt Protein Name
Inward rectifier potassium channel 16
UniProt Gene Name
KCNJ16
UniProt Entry Name
KCJ16_HUMAN
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Product Notes
The KCNJ16 kcnj16 (Catalog #AAA125815) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-KCNJ16 Antibody reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's KCNJ16 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the KCNJ16 kcnj16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KCNJ16, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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