WB (Western Blot)
(Detection of human ASC2 by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASC2 antibody AAA210936 (lot AAA210936-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human ASC2 Polyclonal Antibody | anti-ASC2 antibody
Rabbit anti-ASC2 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ASC2, Antibody; Rabbit anti-ASC2 Antibody, Affinity Purified; TRBP; Thyroid hormone receptor-binding protein; nuclear receptor coactivator RAP250; thyroid hormone receptor binding protein; RAP250; PRIP; PPAR-interacting protein; Peroxisome proliferator-activated receptor-interacting protein; peroxisome proliferator-activated receptor interacting protein; nuclear receptor-activating protein, 250 kDa; amplified in breast cancer protein 3; nuclear receptor coactivator 6; activating signal cointegrator-2; AIB3; amplified in breast cancer-3 protein; ASC2; ASC-2; cancer-amplified transcriptional coactivator ASC-2; NRC; NRC RAP250; Activating signal cointegrator 2; Nuclear receptor coactivator 6; anti-ASC2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ASC2 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat
Immunogen
Between 1075 and 1125
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human ASC2 by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASC2 antibody AAA210936 (lot AAA210936-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human ASC2 by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASC2 antibody AAA210936 (lot AAA210936-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human ASC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASC2 antibody AAA210936 (lot AAA210936-2) used for IP at 6 ug per reaction. ASC2 was also immunoprecipitated by a previous lot of this antibody (lot AAA210936-1) and rabbit anti-ASC2 antibody For blotting immunoprecipitated ASC2, AAA210936 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human ASC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASC2 antibody AAA210936 (lot AAA210936-2) used for IP at 6 ug per reaction. ASC2 was also immunoprecipitated by a previous lot of this antibody (lot AAA210936-1) and rabbit anti-ASC2 antibody For blotting immunoprecipitated ASC2, AAA210936 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human ASC2 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ASC2 (Cat. No. AAA210936 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human ASC2 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ASC2 (Cat. No. AAA210936 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-ASC2 antibody
Activating signal co-integrator 2 (ASC2) functions as a hormone-dependent transcriptional coactivator in cooperation with nuclear receptors such as steroids, retinoids, thyroid hormone, vitamin D3 and prostanoids. ASC2 also interacts with basal transcription factors and may also influence gene expression through its association with histone modifiers. ASC2 has been identified as an amplified gene in breast and other human cancers.
Product Categories/Family for anti-ASC2 antibody
NCBI and Uniprot Product Information
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Product Notes
The ASC2 ncoa6 (Catalog #AAA210936) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ASC2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ASC2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ASC2 ncoa6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ASC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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