IF (Immunofluorescence)
(Immunofluorescent analysis of Asporin staining in rat brain tissues. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Rabbit Asporin Polyclonal Antibody | anti-ASPN antibody
Anti-Asporin Antibody
Gene Names
ASPN; OS3; PLAP1; PLAP-1; SLRR1C
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Purified by Immunogen Affinity Chromatography
Synonyms
Asporin, Antibody; Anti-Asporin Antibody; PLAP1; SLRR1C; Asporin; Periodontal ligament-associated protein 1; PLAP-1; anti-ASPN antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of Asporin protein.
Purity/Purification
Purified by Immunogen Affinity Chromatography
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Applicable Applications for anti-ASPN antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
Recombinant fusion protein of human Asporin. The exact sequence is proprietary.
Preparation and Storage
Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent analysis of Asporin staining in rat brain tissues. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
IF (Immunofluorescence)
(Immunofluorescent analysis of Asporin staining in rat brain tissues. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
WB (Western Blot)
(Western blot analysis of Asporin expression in HeLa (A), SKOV3 (B), mouse stomach (C), rat heart (D) whole cell lysates.)
WB (Western Blot)
(Western blot analysis of Asporin expression in HeLa (A), SKOV3 (B), mouse stomach (C), rat heart (D) whole cell lysates.)
Related Product Information for anti-ASPN antibody
Rabbit polyclonal antibody to Asporin
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,417 Da
NCBI Official Full Name
asporin isoform 2 preproprotein
NCBI Official Synonym Full Names
asporin
NCBI Official Symbol
ASPN
NCBI Official Synonym Symbols
OS3; PLAP1; PLAP-1; SLRR1C
NCBI Protein Information
asporin; asporin proteoglycan; asporin (LRR class 1); small leucine-rich protein 1C; periodontal ligament associated protein 1
UniProt Protein Name
Asporin
UniProt Gene Name
ASPN
UniProt Synonym Gene Names
PLAP1; SLRR1C; PLAP-1
UniProt Entry Name
ASPN_HUMAN
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Product Notes
The ASPN aspn (Catalog #AAA264273) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Asporin Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Asporin can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the ASPN aspn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Asporin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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