Rabbit ATG4C Polyclonal Antibody | anti-ATG4C antibody

Anti-ATG4C Antibody

Cat. Num. AAA292585

• Gene Names: ATG4C; APG4C; AUTL1; AUTL3; APG4-C
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot
• Synonyms: ATG4C, Antibody; Anti-ATG4C Antibody; APG4C; AUTL1; AUTL3; Cysteine protease ATG4C; AUT-like 3 cysteine endopeptidase; Autophagin-3; Autophagy-related cysteine endopeptidase 3; Autophagy-related protein 4 homolog C; anti-ATG4C antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of ATG4C protein.
• Form/Format: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.

• Applicable Applications for anti-ATG4C antibody: IHC (Immunohistochemistry), IC (Immunochemistry), IF (Immunofluorescence), WB (Western Blot)
• Function: Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins (PubMed:21177865, PubMed:29458288, PubMed:30661429). The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine (PubMed:21177865). Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy (By similarity). In addition to the protease activity, also mediates delipidation of ATG8 family proteins (PubMed: ,PubMed: ).Catalyzes delipidation of PE-conjugated forms of ATG8 proteins during macroautophagy(PubMed: ,PubMed: ). Compared to ATG4B, the major protein for proteolytic activation of ATG8 proteins, shows weaker ability to cleave the C-terminal amino acid of ATG8 proteins, while it displays stronger delipidation activity (PubMed: ). In contrast to other members of the family, weakly or not involved in phagophore growth during mitophagy (PubMed:33773106).
• Cellular Location: Cytoplasm {ECO:0000250|UniProtKB:Q8BGE6}.
• Preparation and Storage: Store at -20 °C. Stable for 12 months from date of receipt

IF (Immunofluorescence)

(Immunofluorescent analysis of ATG4C staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of ATG4C staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot)

(Western blot analysis of ATG4C expression in AML12 (A), rat testis (B), MCF7 (C), LO2 (D) whole cell lysates.)

Related Product Information for anti-ATG4C antibody

Rabbit polyclonal antibody to ATG4C
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ATG4C. The exact sequence is proprietary.

NCBI and Uniprot Product Information

• NCBI GI #: 30410844
• NCBI GeneID: 84938
• NCBI Accession #: NP_116241.2
• NCBI GenBank Nucleotide #: NM_032852.3
• UniProt Accession #: Q96DT6; Q96K04; A6NLR8; D3DQ58
• Molecular Weight: 52,497
• NCBI Official Full Name: cysteine protease ATG4C
• NCBI Official Synonym Full Names: autophagy related 4C, cysteine peptidase
• NCBI Official Symbol: ATG4C
• NCBI Official Synonym Symbols: APG4C; AUTL1; AUTL3; APG4-C
• NCBI Protein Information: cysteine protease ATG4C; autophagin-3; APG4 autophagy 4 homolog C; AUT-like 3 cysteine endopeptidase; ATG4 autophagy related 4 homolog C; AUT-like 1, cysteine endopeptidase; autophagy-related protein 4 homolog C; autophagy-related cysteine endopeptidase 3
• UniProt Protein Name: Cysteine protease ATG4C
• UniProt Gene Name: ATG4C
• UniProt Synonym Gene Names: APG4C; AUTL1; AUTL3
• UniProt Entry Name: ATG4C_HUMAN