IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed Hela cells using ATM antibody)
Rabbit ATM Polyclonal Antibody | anti-ATM antibody
ATM antibody
Gene Names
ATM; AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1
Applications
Western Blot, Immunofluorescence
Purity
Purified by immunoaffinity chromatography.
Synonyms
ATM, Antibody; ATM antibody; Rabbit polyclonal ATM antibody; Polyclonal ATM antibody; Anti-ATM antibody; ATE antibody; TEL1 antibody; ATA antibody; ATC antibody; Ataxia telangiectasia mutated antibody; DKFZp781A0353 antibody; TELO1 antibody; AT1 antibody; ATDC antibody; MGC74674 antibody; Ab 1981 antibody; ATD antibody; anti-ATM antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
Purified polyclonal ATM antibody specific for Human ATM.
Purity/Purification
Purified by immunoaffinity chromatography.
Form/Format
Liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl,0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
3056
Applicable Applications for anti-ATM antibody
WB (Western Blot), IF (Immunofluorescence)
Immunogen
ATM antibody was raised in Rabbit using the peptide sequence around aa 1979~1983 (E-G-S-Q-S) derived from Human ATM as the immunogen.
Cross-Reactivity
Human, Mouse
Post-Translational Modifications
ATM is phosphorylated by NUAK1/ARK5. Autophosphorylation occurs on Ser-367, Ser-1893, Ser-1981 and correlates with DNA damage-mediated activation of the kinase. Acetylation, on DNA damage, is required for activation of the kinase activity, dimer-monomer transition, and subsequent autophosphorylation on Ser-198. ATM is acetylated in vitro by KAT5/TIP601. Ubiquitination occurs at Lys215, Lys1109, Lys1114, Lys1126, Lys1323, Lys1572, Lys1994, Lys2025, Lys2148, Lys2160, Lys2789 and Lys2848.
Preparation and Storage
Store at 4 degree C for short term storage.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed Hela cells using ATM antibody)
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed Hela cells using ATM antibody)
WB (Western Blot)
(Western Blot analysis of extracts from 293T cells using ATM antibody and the same antibody preincubated with blocking peptide.)
WB (Western Blot)
(Western Blot analysis of extracts from 293T cells using ATM antibody and the same antibody preincubated with blocking peptide.)
Application Data
(ATM-mediated two-step response to DNA double strand breaks.)
Application Data
(ATM-mediated two-step response to DNA double strand breaks.)
Related Product Information for anti-ATM antibody
Biological Significance: ATM is a serine/threonine protein kinase that is recruited and activated by DNA double-strand breaks. It phosphorylates several key proteins that initiate activation of the DNA damage checkpoint, leading to cell cycle arrest, DNA repair or apoptosis. Several of these targets, including p53, CHK2 and H2AX are tumor suppressors.
Sequence Info: ATM is composed of 3056 amino acids with a molecular weight of 350687 Da. There is a FAT domain at amino acids 1960 2566, a PI3K/PI4K domain at amino acids 2712 2962 and a FATC domain at amino acids 3024 3056.
Implications in Disease: Defects in ATM expression have been closely linked with Ataxia telangiectasia (AT), a rare human disease characterized by cerebellar degeneration, extreme cellular sensitivity to radiation and a predisposition to cancer. All AT patients contain mutations in the ATM gene. Defects in ATM result in severe consequences in repairing certain types of damage to DNA, and cancer may result from improper repair. AT patients have an increased risk for breast cancer that has been ascribed to ATM's interaction and phosphorylation of BRCA1 and its associated proteins following DNA damage. Certain kinds of leukemias and lymphomas, including Mantle cell lymphoma, T-ALL, atypical B cell chronic lymphocytic leukemia, and T-PLL are also associated with ATM defects.
Expression Note: Expression of defective ATM has been reported in a variety of carcinomas. ATM is found in the pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.
Sequence Info: ATM is composed of 3056 amino acids with a molecular weight of 350687 Da. There is a FAT domain at amino acids 1960 2566, a PI3K/PI4K domain at amino acids 2712 2962 and a FATC domain at amino acids 3024 3056.
Implications in Disease: Defects in ATM expression have been closely linked with Ataxia telangiectasia (AT), a rare human disease characterized by cerebellar degeneration, extreme cellular sensitivity to radiation and a predisposition to cancer. All AT patients contain mutations in the ATM gene. Defects in ATM result in severe consequences in repairing certain types of damage to DNA, and cancer may result from improper repair. AT patients have an increased risk for breast cancer that has been ascribed to ATM's interaction and phosphorylation of BRCA1 and its associated proteins following DNA damage. Certain kinds of leukemias and lymphomas, including Mantle cell lymphoma, T-ALL, atypical B cell chronic lymphocytic leukemia, and T-PLL are also associated with ATM defects.
Expression Note: Expression of defective ATM has been reported in a variety of carcinomas. ATM is found in the pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.
Product Categories/Family for anti-ATM antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
350,687 Da
NCBI Official Full Name
Serine-protein kinase ATM
NCBI Official Synonym Full Names
ATM serine/threonine kinase
NCBI Official Symbol
ATM
NCBI Official Synonym Symbols
AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1
NCBI Protein Information
serine-protein kinase ATM
UniProt Protein Name
Serine-protein kinase ATM
UniProt Gene Name
ATM
UniProt Synonym Gene Names
A-T mutated
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Product Notes
The ATM atm (Catalog #AAA224918) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ATM can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence). Researchers should empirically determine the suitability of the ATM atm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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