IHC (Immunohistochemisry)
(DAB staining on fromalin fixed paraffin-embedded Brain tissue))
Rabbit anti-Human, Rat ATP Dependent DNA ligase I Polyclonal Antibody | anti-LIG1 antibody
Polyclonal Antibody to ATP Dependent DNA ligase I (LIG1)
Reactivity
Human, Rat
Applications
ELISA, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Affinity Chromatography
Synonyms
ATP Dependent DNA ligase I, Antibody; Polyclonal Antibody to ATP Dependent DNA ligase I (LIG1); anti-LIG1 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against LIG1. It has beenselected for its ability to recognize LIG1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- KYY GQRAQIHVLE GGEVKIFSRN QEDNSGKYPD IISRIPKIKH PSVTSFILDT EAVAWDREKK QIQPFQVLTT RKRKEVDASE IQVQVCLYAF DLIYLNGESL ARQPLSRRRQ LLRENFVETE GEFVFATSLD TKDIEQIAEF LEQSVKDSCE GLMVKTLDVD ATYEIAKRSH NWLKLKKDYL EGVGDTLDLV VIGAYLGRGK RPGRYGGFLL AAYDEESEEL AAICKLGTGF SDEELEEHHQ NMQALLLPTP RPYVRIDGAV APNHWLDPSI VWEVKCADLT LSPIYRAARG MVDKEKGISL RFPRF
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- KYY GQRAQIHVLE GGEVKIFSRN QEDNSGKYPD IISRIPKIKH PSVTSFILDT EAVAWDREKK QIQPFQVLTT RKRKEVDASE IQVQVCLYAF DLIYLNGESL ARQPLSRRRQ LLRENFVETE GEFVFATSLD TKDIEQIAEF LEQSVKDSCE GLMVKTLDVD ATYEIAKRSH NWLKLKKDYL EGVGDTLDLV VIGAYLGRGK RPGRYGGFLL AAYDEESEEL AAICKLGTGF SDEELEEHHQ NMQALLLPTP RPYVRIDGAV APNHWLDPSI VWEVKCADLT LSPIYRAARG MVDKEKGISL RFPRF
Sequence Length
559
Applicable Applications for anti-LIG1 antibody
ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
Fragment
LIG1 (Lys568~Phe875)
Organism Species
Rattus norvegicus (Rat)
Cross Reactivity
Human, Rat
Conjugate
No Conjugate
Immunogen
Recombinant LIG1 (Lys568~Phe875) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
IHC (Immunohistochemisry)
(DAB staining on fromalin fixed paraffin-embedded Brain tissue))
IHC (Immunohistochemisry)
(DAB staining on fromalin fixed paraffin-embedded Brain tissue))
WB (Western Blot)
(Western Blot: Sample: Recombinant protein.)
WB (Western Blot)
(Western Blot: Sample: Recombinant protein.)
WB (Western Blot)
(Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Rat LIG1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Rat LIG1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
NCBI and Uniprot Product Information
Similar Products
Product Notes
The LIG1 lig1 (Catalog #AAA141853) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to ATP Dependent DNA ligase I (LIG1) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATP Dependent DNA ligase I can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the LIG1 lig1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- KYY GQRAQIHVLE GGEVKIFSRN QEDNSGKYPD IISRIPKIKH PSVTSFILDT EAVAWDREKK QIQPFQVLTT RKRKEVDASE IQVQVCLYAF DLIYLNGESL ARQPLSRRRQ LLRENFVETE GEFVFATSLD TKDIEQIAEF LEQSVKDSCE GLMVKTLDVD ATYEIAKRSH NWLKLKKDYL EGVGDTLDLV VIGAYLGRGK RPGRYGGFLL AAYDEESEEL AAICKLGTGF SDEELEEHHQ NMQALLLPTP RPYVRIDGAV APNHWLDPSI VWEVKCADLT LSPIYRAARG MVDKEKGISL RFPRF. It is sometimes possible for the material contained within the vial of "ATP Dependent DNA ligase I, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.