IHC (Immunohiostchemistry)
(Immunochemical staining of human LIG1 in human duodenum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human LIG1 Polyclonal Antibody | anti-LIG1 antibody
Anti-LIG1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
LIG1, Antibody; Anti-LIG1 Antibody, Rabbit Polyclonal; LIG1 Antibody, Rabbit PAb, Antigen Affinity Purified; anti-LIG1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human LIG1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-LIG1 antibody
IHC (Immunohistochemistry)
Immunogen
E Coli-derived Human LIG1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human LIG1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Immunochemical staining of human LIG1 in human duodenum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human LIG1 in human duodenum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human LIG1 in human lymph node with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human LIG1 in human lymph node with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-LIG1 antibody
DNA ligases catalyze the joining of DNA strands to complete DNA replication, recombination and repair transactions. To protect the integrity of the genome, DNA ligase 1 (LIG1) discriminates against DNA junctions harboring mutagenic 3'-DNA mismatches or oxidative DNA damage. LIG1 is established as the replicative ligase, completing ligation of the >50 million ligation events during DNA replication in addition to ligation events during DNA repair. LIG1 is essential for embryonic development and LIG1 mutants are linked to immunodeficiencies. LIG1 discriminates against 3-mismatches and DNA damage, including oxidative DNA damage incorporated by DNA polymerases. Discrimination by DNA ligases can impair progression of DNA repair when oxidized nucleotides (8oxo-dG, 8oxo-deoxyguanosine) are inserted by DNA repair polymerases. Moreover, the toxicity of anti-cancer MutT homolog 1 (MTH1) inhibitors that increase concentrations of oxidatively damaged nucleotide pools is hypothesized to act in part by promoting DNA repair polymerase incorporation of oxidized nucleotides and subsequent ligation by DNA ligases.
References
Tumbale PP, et al. (2019) Two-tiered enforcement of high-fidelity DNA ligation. Nat Commun 10 (1): 5431.Kori S, et al. (2019) Structure of the uhrf1 tandem tudor domain bound to a methylated non-histone protein, lig1, reveals rules for binding and regulation. Structure 27 (3): 485-496.e487.Hanzlikova H, et al. (2018) The importance of poly(adp-ribose) polymerase as a sensor of unligated okazaki fragments during DNA replication. Mol Cell 71 (2): 319-331.e313.
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Product Notes
The LIG1 (Catalog #AAA256557) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-LIG1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LIG1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the LIG1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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