IP (Immunoprecipitation)
(Detection of human DNA Ligase 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DNA Ligase 1 antibody AAA211331 used for IP at 3 ug/mg lysate. For blotting immunoprecipitated DNA Ligase 1, rabbit anti-DNA Ligase 1 antibody BL4767 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human DNA Ligase 1 Polyclonal Antibody | anti-LIG1 antibody
Rabbit anti-DNA Ligase 1 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
DNA Ligase 1, Antibody; Rabbit anti-DNA Ligase 1 Antibody, Affinity Purified; DNA ligase 1; DNA ligase I; ligase I, DNA, ATP-dependent; polydeoxyribonucleotide synthase [ATP] 1; anti-LIG1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-LIG1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 200 and 250
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human DNA Ligase 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DNA Ligase 1 antibody AAA211331 used for IP at 3 ug/mg lysate. For blotting immunoprecipitated DNA Ligase 1, rabbit anti-DNA Ligase 1 antibody BL4767 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human DNA Ligase 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DNA Ligase 1 antibody AAA211331 used for IP at 3 ug/mg lysate. For blotting immunoprecipitated DNA Ligase 1, rabbit anti-DNA Ligase 1 antibody BL4767 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human DNA Ligase 1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-DNA Ligase 1 (Cat. No. AAA211331) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human DNA Ligase 1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-DNA Ligase 1 (Cat. No. AAA211331) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-LIG1 antibody
DNA ligase 1 catalyzes the joining of double-stranded DNA ends during replication, recombination, and repair. Structurally, DNA ligase 1 is toroidal and encircles nicked DNA substrates. The 9-1-1 complex, a DNA damage checkpoint complex, has been reported to associate with DNA ligase 1 and stimulate ligase activity. Mutations in DNA ligase 1 result in immunodeficiency and increased sensitivity to DNA-damaging agents.
Product Categories/Family for anti-LIG1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
98,228 Da
NCBI Official Full Name
DNA ligase 1 isoform 1
NCBI Official Synonym Full Names
ligase I, DNA, ATP-dependent
NCBI Official Symbol
LIG1
NCBI Protein Information
DNA ligase 1; polydeoxyribonucleotide synthase [ATP] 1
UniProt Protein Name
DNA ligase 1
UniProt Gene Name
LIG1
UniProt Entry Name
DNLI1_HUMAN
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Product Notes
The LIG1 lig1 (Catalog #AAA211331) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-DNA Ligase 1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNA Ligase 1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the LIG1 lig1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA Ligase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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