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IHC (Immunohistochemistry)
(DF5171 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
Rabbit AVPR2 Polyclonal Antibody | anti-AVPR2 antibody
AVPR2 Antibody
Gene Names
AVPR2; DI1; DIR; NDI; V2R; ADHR; DIR3
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Peptide affinity purification
Synonyms
AVPR2, Antibody; AVPR2 Antibody; ADHR; Antidiuretic hormone receptor; Arginine vasopressin receptor 2; AVP R2; AVPR 2; AVPR V2; AVPR2; DI1; DIR 3; DIR; DIR3; MGC126533; MGC138386; NDI; Nephrogenic diabetes insipidus; Renal type arginine vasopressin receptor; Renal-type arginine vasopressin receptor; V2 receptor; V2R; V2R_HUMAN; Vasopressin V2; Vasopressin V2 receptor; anti-AVPR2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
AVPR2 antibody detects endogenous levels of total AVPR2
Purity/Purification
Peptide affinity purification
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
371
Applicable Applications for anti-AVPR2 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human AVPR2, corresponding to a region within the internal amino acids.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohistochemistry)
(DF5171 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
IHC (Immunohistochemistry)
(DF5171 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
IF (Immunofluorescence)
(AAA325769 staining RAW264.7 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA325769 staining RAW264.7 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from rat muscle, using AVPR2 Antibody. Lane 1 was treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of extracts from rat muscle, using AVPR2 Antibody. Lane 1 was treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of extracts from RAW264.7 cells, using AVPR2 antibody.)
WB (Western Blot)
(Western blot analysis of extracts from RAW264.7 cells, using AVPR2 antibody.)
Related Product Information for anti-AVPR2 antibody
Function: Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Involved in renal water reabsorption.
Subunit Structure: Interacts with ARRDC4. Identified in a complex containing at least ARRDC4, V2R and HGS.
Similarity: Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily.
Subunit Structure: Interacts with ARRDC4. Identified in a complex containing at least ARRDC4, V2R and HGS.
Similarity: Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 38 kD
Predicted: 41 kDa
Predicted: 41 kDa
NCBI Official Full Name
vasopressin V2 receptor isoform 1
NCBI Official Synonym Full Names
arginine vasopressin receptor 2
NCBI Official Symbol
AVPR2
NCBI Official Synonym Symbols
DI1; DIR; NDI; V2R; ADHR; DIR3
NCBI Protein Information
vasopressin V2 receptor
UniProt Protein Name
Vasopressin V2 receptor
UniProt Gene Name
AVPR2
UniProt Synonym Gene Names
ADHR; DIR; DIR3; V2R; V2R
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Product Notes
The AVPR2 avpr2 (Catalog #AAA325769) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AVPR2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AVPR2 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the AVPR2 avpr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AVPR2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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